The clinical-chemical application of the NADH test for the determination of diamine oxidase activity in human pregnancy

Kusche, Jürgen; Trotha, U. v.; Mühlberger, G; Lorenz, W.

doi:10.1007/bf01970267

Cited by 9 publications

(3 citation statements)

References 3 publications

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“…Serum DAO activity, however, is raised in pregnancy (Kusche et al 1974), uraemia (Tam et al 1979), kidneydialysis patients (DiSilvestro et al 1997) and after gastrointestinal damage (Wolvekamp & deBruin, 1994), and is reportedly lowered in Crohn's disease and coeliac disease (Bayless et al 1981). Tagaki et al (1994) have questioned the signi®cance of lowered serum DAO activity in these latter diseases as basal activity of serum DAO is very low in normal healthy individuals.…”

Section: Discussionmentioning

confidence: 99%

Response of putative indices of copper status to copper supplementation in human subjects

Kehoe¹,

Turley²,

Bonham³

et al. 2000

Br J Nutr

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No sensitive functional index is currently available to assess Cu status in healthy human populations. This study evaluated the effect of Cu supplementation on putative indices of Cu status in twelve women and twelve men, aged between 22 and 45 years, who participated in a double-blind placebo controlled crossover study. The study consisted of three 6-week supplementation regimens of 3 mg CuSO4, 3 mg Cu-glycine chelate and 6 mg Cu-glycine chelate, each separated by placebo periods of equal length. Women had significantly higher caeruloplasmin oxidase activity (P < 0·001), caeruloplasmin protein concentration (P < 0·05), and serum diamine oxidase activity (P < 0·01) at baseline than men. Erythrocyte and leucocyte superoxide dismutase activity, leucocyte cytochrome c oxidase activity, and erythrocyte glutathione peroxidase activity did not respond to Cu supplementation. Platelet cytochrome c oxidase activity was significantly higher (P < 0·01), after supplementation with 6 mg Cu-glycine chelate in the total group and in women but did not change in men. Caeruloplasmin oxidase activity was significantly higher (P < 0·05), in men after supplementation with 3 mg Cu-glycine chelate, while caeruloplasmin protein concentration was significantly lower in men after supplementation with 6 mg Cu-glycine chelate (P < 0·05). Serum diamine oxidase activity was significantly higher after all supplementation regimens in the total group and in both men and women (P < 0·01). These results indicate that serum diamine oxidase activity is sensitive to changes in dietary Cu intakes and may also have the potential to evaluate changes in Cu status in healthy adult human subjects.

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Section: Discussionmentioning

confidence: 99%

Response of putative indices of copper status to copper supplementation in human subjects

Kehoe¹,

Turley²,

Bonham³

et al. 2000

Br J Nutr

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“…We can assume that the Cu level in the control diet is sufficient to replenish all the pools of Cu in the rat. Knowing that high plasma DAO activities were observed in patients undergoing renal dialysis (DiSilvestro et al 1997), in pregnancy and gestational diseases (Kusche et al 1974) and that DAO activity is also affected by several diseases of the small intestine, the usefulness of this biochemical marker as a diagnostic indicator of marginal Cu deficiency appeared to be limited in the rat or at least questionable. Moreover, preliminary results in our laboratory demonstrated that in healthy human subjects these levels were often nil (Ͻ 1 U/l) making the exploration of Cu deficiency difficult.…”

Section: Discussionmentioning

confidence: 99%

“…DAO is an enzyme catalysing the oxidation of diamines such as histamine, putrescine and cadaverine (Wolvekamp & de Bruin, 1994). It is normally abundant within the intestinal mucosa, kidney and placenta, but scant in serum (Kusche et al 1974;Tam et al 1979;Luk et al 1980;D'Agostino et al 1984). DiSilvestro's group has reported high plasma DAO activities in patients undergoing renal dialysis and in some adult men receiving Cu supplementation, and low activities during spontaneous Cu deficiency (DiSilvestro et al 1997;Jones et al 1997).…”

Section: Copper: Diamine Oxidase: Stable Isotopesmentioning

confidence: 99%

Response of diamine oxidase and other plasma copper biomarkers to various dietary copper intakes in the rat and evaluation of copper absorption with a stable isotope

Feillet‐Coudray¹,

Coudray²,

Bayle³

et al. 2000

Br J Nutr

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There is a lack of agreement on index of Cu status and reliable and sensitive biomarkers are still required. The purpose of this present work was to assess in rats the sensitivity of diamine oxidase (DAO) activity, a recently proposed biomarker, to modifications in dietary Cu intake in comparison with other plasma biomarkers of Cu status. We also evaluated the effect of Cu dietary level on Cu and Zn intestinal absorption. Results showed that plasma Cu and plasma caeruloplasmin were significantly decreased at day 8 compared with the control group (7·4 mg Cu/kg diet) while DAO activity was significantly decreased at day 12 of the deficient diet (0·61 mg Cu/kg diet). Cu supplementation (35 mg Cu/kg diet) had no effect on any of the studied biomarkers of Cu status. In Cu-deficient rats plasma Cu and DAO activities were normalized 4 d after return to the control diet while caeruloplasmin was normalized later, at day 11. Apparent absorption values (%) of total Cu or 65Cu isotope were significantly increased in the Cu-deficient rats compared with the other groups and similar in the control and the Cu-supplemented groups. The urinary excretion of total Cu or 65Cu isotope were increased in the Cu-supplemented group compared with the other two groups. Both apparent absorption and urinary excretion of total Zn or 67Zn isotope remained unchanged in the three experimental groups. In conclusion, DAO activity seemed to be less sensitive to Cu deficiency than plasma Cu or caeruloplasmin concentrations. The present study also showed a significant increase in Cu intestinal absorption with dietary Cu restriction but no decrease with Cu supplementation in the rat.

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Defining Optimal Copper Status in Humans

Strain¹

2002

Trace Elements in Man and Animals 10

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The clinical-chemical application of the NADH test for the determination of diamine oxidase activity in human pregnancy

Cited by 9 publications

References 3 publications

Response of putative indices of copper status to copper supplementation in human subjects

Response of putative indices of copper status to copper supplementation in human subjects

Response of diamine oxidase and other plasma copper biomarkers to various dietary copper intakes in the rat and evaluation of copper absorption with a stable isotope

Defining Optimal Copper Status in Humans

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