The coat protein of Indian peanut clump virus: relationships with other furoviruses and with barley stripe mosaic virus

Wesley, S. V.; Mayo, M. A.; Jolly, Caroline; Naidu, R. A.; Reddy, D. V. R.; Jana, M. K.; Parnaik, Veena K.

doi:10.1007/bf01310566

Cited by 18 publications

(16 citation statements)

References 23 publications

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“…Transformation of plants with DNA encoding the coat protein and replicase genes of viruses is an efficient means to enhance the germplasm for resistance to viral diseases [19]. The coat protein gene of IPCV from its RNA-2 has been cloned and sequenced [20] and its expression in Nicotiana benthamiana studied [21]. At ICRISAT, attempts are underway to induce resistance to IPCV and other peanut viruses by using biotechnological tools.…”

Section: Introductionmentioning

confidence: 99%

An efficient method for the production of transgenic plants of peanut (Arachis hypogaea L.) through Agrobacterium tumefaciens-mediated genetic transformation

2000

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Cotyledon explants from mature peanut seeds (Arachis hypogaea L.) were optimized to obtain adventitious shoot buds with high frequencies (>90%). Efficient transformation of these cotyledons by using Agrobacterium tumefaciens strain C58 carrying neomycin phosphotransferase II (nptII) and ß-glucuronidase (GUS; uidA), or coat protein gene of the Indian peanut clump virus (IPCVcp) and nptII on binary vectors (pBI121; pROKII:IPCVcp) led to the production of a large percentage (55%) of transgenic plants. Transformed individuals were obtained through selection on medium containing 125 mg l(-1) kanamycin. A large number of independently transformed plants (over 75) were successfully transplanted to the glasshouse. Integration of the transgenes and stable genetic transformants in the progeny were assessed by PCR amplification of 700-bp fragment of nptII and 585-bp of IPCVcp genes, and Southern blot hybridizations in the T1 generation of transgenic plants. Analysis of 35 transgenic plants of T1 generation from the progeny of a single transformation event suggested the segregation of a single copy insert in a 3:1 Mendelian ratio. On an average, 120-150 days were required between the initiation of explant transformation and transfer of rooted plants to the greenhouse. The cotyledon regeneration system proved to be an excellent vehicle for the production of a large number of independently transformed peanut plants. Shoot formation was rapid and prolific, and a large proportion of these shoots developed into fertile plants. The method reported here provides new opportunities for the crop improvement of peanut via genetic transformation.

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Section: Introductionmentioning

confidence: 99%

An efficient method for the production of transgenic plants of peanut (Arachis hypogaea L.) through Agrobacterium tumefaciens-mediated genetic transformation

2000

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“…There is some sequence homology among these species for PCV, BSMV and SBWMV, but less with the M r 14000 species of BNYVV. It should also be noted that there is considerable homology between the coat proteins of PCV and BSMV (Wesley et al, 1994). Finally, the three furoviruses possess one feature in common which is not shared with BSMV: the presence on RNA 2 of an additional ORF immediately following the Y-proximal coat protein cistron.…”

Section: Relationships With Other Furovirusesmentioning

confidence: 99%

“…There is a second probable furovirus of groundnut, Indian peanut clump virus (IPCV), which has rod-shaped virions of similar size to those of PCV and an apparently bimodal genome (Reddy et al, 1983). Although PCV and IPCV are not serologically related, recent sequence analysis (Wesley et al, 1994) has revealed that the coat protein of IPCV is 61% identical to that of PCV.…”

Section: Introductionmentioning

confidence: 99%

Complete Nucleotide Sequence of Peanut Clump Virus RNA 1 and Relationships With Other Fungus-transmitted Rod-shaped Viruses

Herzog

Guilley

Manohar

et al. 1994

Journal of General Virology

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The complete nucleotide sequence of RNA 1 of the tentative furovirus peanut clump virus (PCV) has been determined by characterization of cloned cDNA and by direct RNA sequencing. The sequence is 5897 nucleotides in length and contains three long open reading frames (ORFs). The Y-terminal proximal ORF has the potential to encode a polypeptide of M r 130942 (P131) containing methyltransferase and RNA helicase homologous domains and displaying homology with large nonstrnctural proteins of alpha-like viruses, which are known or thought to be involved in virus replication. The P131 ORF is followed in-frame by a second ORF which is probably expressed by partial readthrough of the UGA termination codon of the P131 ORF to produce a polypeptide of M r 191044 (P191). The readthrough region of P191 contains the characteristic 'core' RNA polymerase motif, indicating that the PCV replicase proteins are expressed as a pair of overlapping proteins as in the tobamoviruses, tobraviruses and the furovirus soil-borne wheat mosaic virus (SBWMV). Sequence comparisons indicate that P 131 and P 191 are most closely related to the replicase proteins of SBWMV and the hordeivirus barley stripe mosaic virus (BSMV) but are only distantly related to the replicase of the furovirus beet necrotic yellow vein virus (BNYVV). The T-terminal proximal ORF can encode a putative polypeptide ofM r 14556 (P15) which displays homology to small cysteine-rich proteins of hordeivirnses and SBWMV. We have corrected four errors in the sequence of PCV RNA 2 published previously by Manohar et al. (Virology 195, 33-41, 1993). One of these changes causes two small ORFs near the 3' terminus of RNA 2 to be fused together to create an ORF for a putative polypeptide ofM r 16 833 (P17) which displays extensive homology with the third protein of the triple gene block of BSMV RNA fl.

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“…Symptoms on wheat are similar to those caused by SBWMV. However, there is no serological relationship between the two viruses (Reddy et al, 1985) and their genome organization differs substantially (Wesley et al, 1994;Miller et al, 1996;Naidu et al, 1996). The yield reduction in wheat infected with IPCV-H was very severe and consistent over the 3-year period.…”

Section: Discussionmentioning

confidence: 99%

Indian peanut clump virus (IPCV) infection on wheat and barley: symptoms, yield loss and transmission through seed

et al. 1999

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Wheat and barley crops were shown to be susceptible to Indian peanut clump virus (IPCV) under field conditions. In wheat, the Hyderabad isolate of IPCV (IPCV-H) induced symptoms resembling the rosette caused by soil-borne wheat mosaic virus, and these were apparent only three weeks after emergence. Early-infected plants were severely stunted and dark green, with chlorotic streaks on the youngest leaves, which turned necrotic as the plants aged; most of these plants died. Late-infected plants were also stunted and were conspicuous in the field because of their dark green appearance as a result of delayed maturity. The virus was detected by ELISA and nucleic acid hybridization in all plants with symptoms. These plants usually produced fewer tillers than healthy ones. Spikes were malformed, often did not emerge from the flag leaf, and they contained few, shrivelled seeds. Grain yield was decreased, on average, by 58%. In barley, IPCV-H caused severe stunting and general leaf chlorosis. As the plants aged, the leaves became necrotic and the few infected plants that reached maturity produced small spikes. IPCV-H antigens were detected by ELISA in every wheat seed from infected plants and the virus was transmitted through wheat seed at a frequency of 0·5-1·3%. Storage at 4ЊC for more than a year did not affect seed transmission frequency. The virus was detected in leaves and roots of seed-transmitted plants. Seed transmission was not detected in barley. The Durgapura isolate (IPCV-D) was detected in wheat crops (cv. RR-21) at 3 different locations in Rajasthan State, India. Infected plants showed reduced growth without any overt symptoms.

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The coat protein of Indian peanut clump virus: relationships with other furoviruses and with barley stripe mosaic virus

Cited by 18 publications

References 23 publications

An efficient method for the production of transgenic plants of peanut (Arachis hypogaea L.) through Agrobacterium tumefaciens-mediated genetic transformation

An efficient method for the production of transgenic plants of peanut (Arachis hypogaea L.) through Agrobacterium tumefaciens-mediated genetic transformation

Complete Nucleotide Sequence of Peanut Clump Virus RNA 1 and Relationships With Other Fungus-transmitted Rod-shaped Viruses

Indian peanut clump virus (IPCV) infection on wheat and barley: symptoms, yield loss and transmission through seed

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