The complete genome sequence of Exiguobacterium arabatum W‐01 reveals potential probiotic functions

Abstract

Shrimp is extensively cultured worldwide. Shrimp farming is suffering from a variety of diseases. Probiotics are considered to be one of the effective methods to prevent and cure shrimp diseases. Exiguobacterium arabatum W‐01, a gram‐positive and orange‐pigmented bacterium, was isolated from the intestine of a healthy Penaeus vannamei specimen. Whole‐genome sequencing revealed a genome of 2,914,854 bp, with 48.02% GC content. In total, 3,083 open reading frames (ORFs) were identified, with an average length of 843.98 bp and a mean GC content of 48.11%, accounting for 89.27% of the genome. Among these ORFs, 2,884 (93.5%) genes were classified into Clusters of Orthologous Groups (COG) families comprising 21 functional categories, and 1,650 ORFs were classified into 83 functional Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. A total of 27 rRNA operons and 68 tRNAs were identified, with all 20 amino acids represented. In addition, 91 genomic islands, 68 potential prophages, and 33 tandem repeats, but no clustered regularly interspaced short palindromic repeats (CRISPRs), were found. No resistance genes and only one virulence gene were identified. Among the 150 secreted proteins of E. arabatum W‐01, a variety of transport system substrate‐binding proteins, enzymes, and biosynthetic proteins, which play important roles in the uptake and metabolism of nutrients, were found. Two adherence‐related protein genes and 31 flagellum‐related protein genes were also identified. Taken together, these results indicate potential probiotic functions for E. arabatum W‐01.

Screening and Whole-Genome Sequencing of TwoStreptomycesSpecies from the Rhizosphere Soil of Peony Reveal Their Characteristics as Plant Growth-Promoting Rhizobacteria

“…1 (GA) [ 27 ] medium with 3% potassium dichromate, and then the plates were incubated at 28°C for 4-7 days. The dominant colonies were isolated and then triply streaked to obtain the absolute single colonies [ 28 ]. F. solani , which is the pathogen of peony root rot was cultivated using potato dextrose agar (PDA) [ 27 ] medium at 28°C.…”

Modulation of the gut microbiota of Pacific white shrimp ( Penaeus vannamei Boone, 1931) by dietary inclusion of a functional yeast cell wall‐based additive

“…On the contrary, one‐third of the OTUs were found exclusively within the gut microbiota of animals that received the YCW, including Exiguobacterium and Vibrio . Exiguobacterium has been proposed to be a potential probiotic for P. vannamei (Cong et al, 2017) and may increase shrimp survival and growth (Sombatjinda et al, 2014). Moreover, this genus has potential biotechnological use to industry and agriculture (Kasana & Pandey, 2018).…”

Distinct Clinical Pathology and Microbiota in Chronic Rhinosinusitis With Nasal Polyps Endotypes

“…48 Exiguobacterium arabatum W01 has potential probiotic function. 49 Lawsonella clevelandesis is a common nasal commensal. 50 These bacterial species seem to prevent inflammation.…”

“…is a member of the human microbiota and is rarely implicated in infections 48 . Exiguobacterium arabatum W01 has potential probiotic function 49 . Lawsonella clevelandesis is a common nasal commensal 50 .…”