The complete mitochondrial genome of <i>Ixodes vespertilionis</i> (Acari: Ixodidae)

Lu, Xinyan; Zuo, Xiuhua; Jiang, Dandan; Xing, Yang

doi:10.1080/23802359.2021.1976686

Cited by 7 publications

(5 citation statements)

References 14 publications

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“…rufipes , Rhipicephalus microplus , and many soft tick mitogenomes [ 37 , 38 , 62 , 77 , 78 , 79 , 80 , 81 ]. Although Lu et al reported that no sequence identical to the Tick-Box consensus motif was present in I. vespertilionis mitogenome [ 82 ], we successfully found the motif within trnL1 (5′-TTGTATCAAATTTAGAA-3′) and downstream of ND1 (5′-TTGTATCCTTTT G AGAA-3′) on the light chain.…”

Section: Resultsmentioning

confidence: 82%

Complete Mitogenomes of Ticks Ixodes acutitarsus and Ixodes ovatus Parasitizing Giant Panda: Deep Insights into the Comparative Mitogenomic and Phylogenetic Relationship of Ixodidae Species

Liu

et al. 2022

Genes

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Ticks rank second in the world as vectors of disease. Tick infestation is one of the factors threatening the health and survival of giant pandas. Here, we describe the mitogenomes of Ixodes acutitarsus and Ixodes ovatus parasitizing giant pandas, and perform comparative and phylogenetic genomic analyses on the newly sequenced and other available mitogenomes of hard ticks. All six newly determined mitogenomes contain a typical gene component and share an ancient Arthropoda gene arrangement pattern. Our study suggests that I. ovatus is a species complex with high genetic divergence, indicating that different clades of I. ovatus represent distinct species. Comparative mitogenomic analyses show that the average A + T content of Ixodidae mitogenomes is 78.08%, their GC-skews are strongly negative, while AT-skews fluctuate around 0. A large number of microsatellites are detected in Ixodidae mitogenomes, and the main microsatellite motifs are mononucleotide A and trinucleotide AAT. We summarize five gene arrangement types, and identify the trnY-COX1-trnS1-COX2-trnK-ATP8-ATP6-COX3-trnG fragment is the most conserved region, whereas the region near the control region is the rearrangement hotspot in Ixodidae mitogenomes. The phylogenetic trees based on 15 genes provide a very convincing relationship (Ixodes + (Robertsicus + ((Bothriocroton + Haemaphysalis) + (Amblyomma + (Dermacentor + (Rhipicentor + (Hyalomma + Rhipicephalus))))))) with very strong supports. Remarkably, Archaeocroton sphenodonti is embedded in the Haemaphysalis clade with strong supports, resulting in paraphyly of the Haemaphysalis genus, so in-depth morphological and molecular studies are essential to determine the taxonomic status of A. sphenodonti and its closely related species. Our results provide new insights into the molecular phylogeny and evolution of hard ticks, as well as basic data for population genetics assessment and efficient surveillance and control for the giant panda-infesting ticks.

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Section: Resultsmentioning

confidence: 82%

Complete Mitogenomes of Ticks Ixodes acutitarsus and Ixodes ovatus Parasitizing Giant Panda: Deep Insights into the Comparative Mitogenomic and Phylogenetic Relationship of Ixodidae Species

Liu

et al. 2022

Genes

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“…Adult samples of H. yeni were collected in March 2021 from High Li Gong Shan in the Nujiang Lisu Autonomous Prefecture of the Chinese Yunnan Province (26°34′N, 98°48′E) ( n = 2, female; n = 1, male). Professor Chunhong Du examined the species' morphological identification using the essential diagnostic features ( 13 ). After collection, one adult female specimen was used for DNA extraction, and the remainder of the ticks were held as voucher specimens.…”

Section: Methodsmentioning

confidence: 99%

“…The samples were maintained at −80°C and preserved in 95% alcohol before being utilized for DNA extraction. Single tick genomic DNA was extracted using DNAzol (Life Technologies, USA) following the manufacturer's instructions and stored for further processing ( 13 ).…”

Section: Methodsmentioning

confidence: 99%

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Complete mitogenomes and phylogenetic relationships of Haemaphysalis nepalensis and Haemaphysalis yeni

Zhang

Jiang

et al. 2022

Front. Vet. Sci.

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The mitochondrial genome may include crucial data for understanding phylogenetic and molecular evolution. We sequenced the complete mitogenome of Haemaphysalis nepalensis and Haemaphysalis yeni for the first time. H. nepalensis and H. yeni's complete mitogenomes were 14,720 and 14,895 bp in size, respectively, and both contained two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and 13 protein-coding genes (PCG). Haemaphysalis nepalensis have one control region (D-loop). The adenine + thymine concentration of the genomes of H. nepalensis and H. yeni was 77.75 and 78.41%, respectively. The codon use pattern and amino acid content of proteins were both observed to be affected by the AT bias. Genes in the mitogenome were organized and located in a comparable manner to previously known genes from Haemaphysalis ticks. Mitochondrial PCGs were used to perform phylogenetic relationships based on the Minimum Evolution (ME) approach using MEGA 7.0 software, the results reveal that H. nepalensis has tight links with H. tibetensis, H. yeni and H. kolonini share a sister group relationship, and that H. nepalensis and H. yeni belong to Haemaphysalis. The results of this study include the following: (i) discovered and supplied new tick records (H. nepalensis) for China, (ii) provided the first complete mitochondrial genome for H. nepalensis and H. yeni and revealed their phylogenetic relationships, and (iii) the features of the mitochondrial genome of H. nepalensis and H. yeni provided more genetic reference for Phylogeography, systematics, and population genetics of the Haemaphysalis species.

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“…The entire DNA was extracted using the standard CTAB technique, for storage at −20 °C before use (Lu et al. 2021 ). The mitochondrial genome was sequenced on the Illumina NovaSeq platform (Shanghai Personal Biotechnology Co, Ltd) which was assembled using A5-miseq software (Coil et al.…”

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confidence: 99%

Complete mitochondrial genome and phylogenetic analysis of Ixodes ovatus (Acari: Ixodidae)

Jiang

et al. 2022

Mitochondrial DNA Part B

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Ixodes ovatus is referred to as an obligatory blood-sucking ectoparasite that is capable to infest both humans and animals. In the present study, the complete mitochondrial genome of I. ovatus was sequenced and analyzed using next-generation sequencing (NGS) technology. With a size of 14,520 bp, the entire mitogenome contains 37 genes including 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs), and 3 control regions (D-loops). Based on the 13 PCGs nucleotide sequences, the phylogenetic relationship of I. ovatus was analyzed using Maximum-likelihood. As suggested by the results of the obtained phylogenety, I. ovatus is most closely associated with Ixodes hexagonus. This study is expected to promote further studies on the evolution of Ixodidae.

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The complete mitochondrial genome of Ixodes vespertilionis (Acari: Ixodidae)

Cited by 7 publications

References 14 publications

Complete Mitogenomes of Ticks Ixodes acutitarsus and Ixodes ovatus Parasitizing Giant Panda: Deep Insights into the Comparative Mitogenomic and Phylogenetic Relationship of Ixodidae Species

Complete Mitogenomes of Ticks Ixodes acutitarsus and Ixodes ovatus Parasitizing Giant Panda: Deep Insights into the Comparative Mitogenomic and Phylogenetic Relationship of Ixodidae Species

Complete mitogenomes and phylogenetic relationships of Haemaphysalis nepalensis and Haemaphysalis yeni

Complete mitochondrial genome and phylogenetic analysis of Ixodes ovatus (Acari: Ixodidae)

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