The complete mitochondrial genome of Metorchis orientalis (Trematoda: Opisthorchiidae): Comparison with other closely related species and phylogenetic implications

Lü, Na; Gao, Jinliang; Li, Guohua; Fu, Xue; Su, Xin; Yue, Dongmei; Gao, Yuan; Zhang, Yan; Wang, Chun-Ren

doi:10.1016/j.meegid.2016.01.010

Cited by 42 publications

(22 citation statements)

References 29 publications

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“…xanthosomus , and E . pancreaticus since they are closely related and cannot be easily differentiated with molecular method [27–30]. Understanding of the biology, distribution, transmission and factors influencing O .…”

Section: Discussionmentioning

confidence: 99%

First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar

Aung

Htoon²,

Tin³

et al. 2017

PLoS ONE

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Opisthorchis viverrini is endemic in the South East Asian region, especially in Cambodia, Lao People's Democratic Republic, Vietnam and Thailand, but there have been no previous records from Myanmar. During stool surveys of rural populations in three regions of Lower Myanmar, Opisthorchis-like eggs were found in 34 out of 364 (9.3%) participants by stool microscopy after using the modified formalin-ether concentration technique. DNA was extracted from these positive stool samples and a portion of the mitochondrial cytochrome c oxidase subunit I (cox1) gene was amplified using the polymerase chain reaction and then sequenced. DNA sequences, successfully obtained from 18 of 34 positive samples (Bago Region, n = 13; Mon State, n = 3; Yangon Region, n = 2), confirmed that the eggs were of O. viverrini. Sequences showed 99.7% identity with O. viverrini mitochondrial cox1 (GenBank accession no. JF739555) but 95%, 88.7%, 82.6% and 81.4% identities with those of Opisthorchis lobatus from Lao People's Democratic Republic (GenBank accession nos. HQ328539-HQ328541), Metorchis orientalis from China (KT239342), Clonorchis sinensis from China (JF729303) and Opisthorchis felineus from Russia (EU921260), respectively. When alignement with other Opisthorchiidae trematodes, 81% similarity with Metorchis bilis from Czech Republic (GenBank accession nos. KT740966, KT740969, KT740970) and Slovakia (GenBank accession nos. KT740971-KT740973), 84.6% similarity with Metorchis xanthosomus from Czech Republic (GenBank accession no. KT740974), 78.6% similarity with M. xanthosomus from Poland (GenBank accession no. KT740968) and 82.2% similarity with Euamphimerus pancreaticus from Czech Republic (GenBank accession no. KT740975) were revealed. This study demonstrated, for the first time, O. viverrini from rural people in Myanmar using molecular methods and is an urgent call for surveillance and control activities against opisthorchiasis in Myanmar.

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Section: Discussionmentioning

confidence: 99%

First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar

Aung

Htoon²,

Tin³

et al. 2017

PLoS ONE

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“…Recently Na et al . () compared the mitochondrial genome of the opisthorchiid Metorchis orientalis with other digeneans and, the structure and organization of the gene arrangements demonstrated to be highly conserved among species of Opisthorchiidae, Heterophyidae and Fasciolidae. Their phylogenetic analysis showed monophyletic groupings for all the families used, including the Heterophyidae.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and confocal laser scanning microscopic study of Pygidiopsis macrostomum (Trematoda: Heterophyidae) parasites of guppies Poecilia vivipara

Borges

Costa

Mantovani

et al. 2016

Journal of Fish Diseases

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Pygidiopsis macrostomum and Ascocotyle (Phagicola) pindoramensis (Digenea: Heterophyidae) parasitize guppies as intermediate hosts and, respectively, fish‐eating mammals or birds as definitive hosts. Heterophyids have zoonotic potential, and molecular studies associated with morphological and ecological aspects have helped to clarify their taxonomy and phylogeny. Poecilia vivipara naturally parasitized by metacercariae of both species (100% prevalence) exhibit no external signs of parasitism. In this work, four new sequences of P. macrostomum (18S rDNA, 28S rDNA and ITS2 rDNA) and one new sequence of A. (P.) pindoramensis (mtDNA cox‐1) are presented. Phylogeny reconstructions linked P. macrostomum to other heterophyids, but the separation of the Heterophyidae and Opisthorchiidae remains unclear. Additionally, we used indirect immunocytochemistry and the phalloidin‐fluorescence techniques allied with confocal laser scanning microscopy to describe muscular and neuronal structures of P. macrostomum. A complex arrangement of muscular fibres is associated with the tegument, suckers, gut and reproductive system. Radial fibres around the ventral sucker are thick, branched and extend to the body wall. High‐resolution confocal imaging revealed a typical digenean muscular arrangement and important heterophyid morphological traits. These data will support future control measures to reduce the parasitism in guppies reared in fish farming systems, especially for aquarium and experimental purposes.

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“…BD2013 PCcercaBD Vietnam MF287771–MF287773 This study Opisthorchis sp. BD2013 PCeggBD Vietnam MF287774–MF287776 This study Opisthorchis viverrini na Laos b JF739555 [ 19 ] Binh Dinh 1 Vietnam b MF287777–MF287779 This study Khon Kaen Thailand b MF287780–MF287782 This study Opisthorchis felineus Ust-Tula (Novosibirsk) Russia b EU921260 [ 16 ] Clonorchis sinensis Nam Dinh Vietnam c MF287783–MF287785 This study Guangdong China b JF729303 [ 19 ] na South Korea b JF729304 [ 19 ] Amur - Khabarovsk Russia b FJ381664 [ 16 ] Metorchis orientalis Heilongjiang China b KT239342 [ 22 ] Heterophyidae Haplorchis taichui na Laos KF214770 [ 24 ] Quang Tri 3 Vietnam MF287786–MF287788 This study Metagonimus yokogawai na South Korea KC330755 Fasciolidae Fasciola hepatica Geelong ...…”

Section: Methodsmentioning

confidence: 99%

Updated molecular phylogenetic data for Opisthorchis spp. (Trematoda: Opisthorchioidea) from ducks in Vietnam

et al. 2017

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BackgroundAn opisthorchiid liver fluke was recently reported from ducks (Anas platyrhynchos) in Binh Dinh Province of Central Vietnam, and referred to as “Opisthorchis viverrini-like”. This species uses common cyprinoid fishes as second intermediate hosts as does Opisthorchis viverrini, with which it is sympatric in this province. In this study, we refer to the liver fluke from ducks as “Opisthorchis sp. BD2013”, and provide new sequence data from the mitochondrial (mt) genome and the nuclear ribosomal transcription unit. A phylogenetic analysis was conducted to clarify the basal taxonomic position of this species from ducks within the genus Opisthorchis (Digenea: Opisthorchiidae).MethodsAdults and eggs of liver flukes were collected from ducks, metacercariae from fishes (Puntius brevis, Rasbora aurotaenia, Esomus metallicus) and cercariae from snails (Bithynia funiculata) in different localities in Binh Dinh Province. From four developmental life stage samples (adults, eggs, metacercariae and cercariae), the complete cytochrome b (cob), nicotinamide dehydrogenase subunit 1 (nad1) and cytochrome c oxidase subunit 1 (cox1) genes, and near-complete 18S and partial 28S ribosomal DNA (rDNA) sequences were obtained by PCR-coupled sequencing. The alignments of nucleotide sequences of concatenated cob + nad1 + cox1, and of concatenated 18S + 28S were separately subjected to phylogenetic analyses. Homologous sequences from other trematode species were included in each alignment.ResultsPhylogenetic trees were inferred from concatenated (cob + nad1 + cox1) nucleotide sequences and combined 18S + 28S nucleotide sequences of five Opisthorchis sp. BD2013 samples and additional reference taxa. Both trees demonstrated the anticipated clustering of taxa within the superfamily Opisthorchioidea, the paraphyly of the genus Opisthorchis and the sister-species relationship of Opisthorchis sp. BD2013 with O. viverrini.ConclusionsWhile it is likely that Opisthorchis sp. BD2013 is distinct from O. viverrini, it is clearly a sister taxon of O. viverrini within the limited number of Opisthorchis species for which appropriate sequence data are available. The new sequences provided here will assist the diagnosis and the taxonomic clarification of the opisthorchiid species.

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The complete mitochondrial genome of Metorchis orientalis (Trematoda: Opisthorchiidae): Comparison with other closely related species and phylogenetic implications

Cited by 42 publications

References 29 publications

First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar

First report and molecular identification of Opisthorchis viverrini infection in human communities from Lower Myanmar

Molecular characterization and confocal laser scanning microscopic study of Pygidiopsis macrostomum (Trematoda: Heterophyidae) parasites of guppies Poecilia vivipara

Updated molecular phylogenetic data for Opisthorchis spp. (Trematoda: Opisthorchioidea) from ducks in Vietnam

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