The complete nucleotide sequence and genome organization of odontoglossum ringspot tobamovirus RNA

Abstract

The complete nucleotide sequence of the genomic RNA of odontoglossum ringspot tobamovirus (ORSV) was determined. The RNA genome of ORSV is 6618 nucleotides long and contains five open reading frames (ORFs 1 to 5) coding for proteins of M(r) 126 K, 181 K, 34 K, 18 K and 52 K, respectively. This is the longest RNA of the known viruses of the Tobamovirus genus. The sequences of the ORSV RNA encoded proteins exhibit high homology to the proteins of the members of the Tobamovirus genus. The genomic organization and sequence analysis showed that ORSV is more closely related to tobacco mild green mosaic virus (TMGMV), pepper mild mottle virus (PMMV), tomato mosaic virus (ToMV) and TMV than to cucumber green mottle mosaic virus (CGMMV) and sunn-hemp mosaic virus (SHMV).

High sequence conservation among Odotoglossum ringspot virus isolates from orchids

“…Similarly, a study with a collection of various TMGMV geographic populations from Nicotiana glauca showed that nt diversity values were smaller than those reported for other RNA genomes [18]. In accordance with these observations, the clustering of ORSV isolates did not correlate with the geographic origin or with the host and confirmed previous CP analyses data for the tree obtained from the CP alignment [5,10]. In addition, our analyses with nt and aa sequences of CP gene of 86 global isolates suggested the ORSV genome is highly conserved, similar to TMV and Pepper mild mottle virus [17,19,20,22].…”

“…ORSV-infected orchids often show a variety of symptoms such as color-breaking in flowers, diamond mottle, streak or stripe mosaic or necrotic spots, yellowing leaves, as well as symptomless conditions [3,6,9]. To date, the complete nucleotide (nt) sequences of three ORSV isolates have been reported [10][11][12], and the complete nt sequences for only two Taiwan isolates of ORSV are available in the GenBank database (accession numbers DQ139262 and AY571290). These studies using phylogenetic tree analysis demonstrated evolutionary convergence in RNA-dependent RNA polymerase, movement protein, and coat protein (CP) within each virus clusters.…”

Introduction of Odontoglossum ringspot virus Coat Protein Gene into Cymbidium niveo-marginatum mediated by Agrobacterium tumefaciens to Produce Transgenic Plants

“…Furthermore the sequences of the 477 bp from the start to stop codons of all 9 colonies were identical. In this range, the correspondences were 99.58%, 99.37%, 99.37%, 99.79%, and 99.58% with the sequences isolated in France (Type-Strain, called it as F TS , Dubs and Van Regenmortel, 1990), Japan (Cy-1, J Cy-1 , Isomura et al, 1991), the U.S. (TMV-O, A TMV-O , Hilf and Dawson, 1993), Korea (Cy, K Cy , Ryu et al, 1995), and Singapore (S 1 , Chng et al, 1996), respectively (Fig. 4A).…”

“…. J E , analyzed by us in this time isolated from an ORSV-infected Epidendrum in Japan; J Cy-1 , reported by Isomura et al (1991) isolated from Cy-1 strain in Japan; S 1 , reported by Chng et al (1996) isolated from a Singapore strain; K Cy , reported by Ryu et al (1995) isolated from Cy strain in Korea; F TS , reported by Dubs and Van Regenmortel (1990) Confirming the expression of GFP by using a fluorescent microscope The GFP was observed in 52 of 81 (64%) surviving rhizomes, whereas it was observed in 84 of 215 (39%) of the regenerated shoots. The expression of GFP appeared strong in 5 rhizome sections and shoots that were induced from these rhizomes.…”

“…The CP gene of ORSV has already been sequenced (Ajjikuttira et al, 2002;Chng et al, 1996;Dubs and Van Regenmortel, 1990;Hilf and Dawson, 1993;Ikegami et al, 1995;Isomura et al, 1991;Ryu and Park, 1995). However, there has been no report on the CPmediated transgenic orchidaceous plants.…”