The composition of the cuticular lipids from nymphs and exuviae of the Silverleaf Whitefly, Bemisia argentifolii

Buckner, James S.; Hagen, Marcia M.; Nelson, Dennis R.

doi:10.1016/s0305-0491(99)00117-0

Cited by 44 publications

(35 citation statements)

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“…In insect cuticular lipids, the following alcohols have been identified: C 22:0 to C 32:0 in Melanoplus sanguinipes and Melanoplus packardii adults (Soliday et al, 1974), C 28:0 to C 34:0 in Bemisia argentifolii nymphs (Buckner et al, 1999), C 24:0 to C 34:0 in Heliothis virescens pupae and Aleyrodes singularis exuviae (Buckner et al, 1996;Nelson et al, 1998), C 26:0 to C 34:0 in Helicoverpa zea pupae (Buckner et al, 1996), C 22:0 to C 34:0 in A. singularis adults (Nelson et al, 1998), C 8:0 to C 22:0 in Acyrthosiphon pisum (Brey et al, 1985), C 32:0 and C 34:0 in Bemisia tabaci adults (Buckner et al, 1994), C 30:0 and C 32:0 in Trialeurodes vaporariorum adults (Buckner et al, 1994), C 12:0 to C 34:0 in Locusta migratoria migratoriodes adults (Oraha and Lockey, 1990) and C 12:0 to C 32:0 in Schistocerca gregaria adults (Oraha and Lockey, 1990) (Table9). In the present study, the cuticular lipids of M. domestica males and females contained alcohols from C 12:0 to C 28:0 and those of larvae and pupae contained alcohols from C 12:0 to C 30:0 .…”

Section: Discussionmentioning

confidence: 99%

The antimicrobial activity of the alcohols fromMusca domestica

Gołębiowski

Dawgul

Kamysz

et al. 2012

Journal of Experimental Biology

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SUMMARYInformation on the stimulatory and inhibitory effects of cuticular alcohols on growth and virulence of insecticidal fungi is unavailable. Therefore, we set out to describe the content of cuticular and internal alcohols in the body of housefly larvae, pupae, males and females. The total cuticular alcohols in larvae, males and females of Musca domestica were detected in comparable amounts (4.59, 3.95 and 4.03gg -1 insect body, respectively), but occurred in smaller quantities in pupae (2.16gg -1). The major free alcohol in M. domestica larvae was C 12:0 (70.4%). Internal alcohols of M. domestica larvae were not found. Among cuticular pupae alcohols, C 12:0 (31.0%) was the most abundant. In the internal lipids of pupae, only five alcohols were identified in trace amounts. The most abundant alcohol in males was C 24:0 (57.5%). The percentage content of cuticular C 24:0 in males and females (57.5 and 36.5%, respectively) was significantly higher than that of cuticular lipids in larvae and pupae (0.9 and 5.6%, respectively). , respectively). For isolated alcohols, antimicrobial activity against 10 reference strains of bacteria and fungi was determined. Individual alcohols showed approximately equal activity against fungal strains. C 14:0 was effective against gram-positive bacteria, whereas gram-negative bacteria were resistant to all tested alcohols. Mixtures of alcohols found in cuticular lipids of larvae, pupae, males and females of M. domestica generally presented higher antimicrobial activity than individual alcohols. In contrast, crude extracts containing both cuticular and internal lipids showed no antifungal activity against the entomopathogenic fungus Conidiobolus coronatus, which efficiently kills adult house flies. Only two alcohols were present in the internal lipids of males in trace amounts

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Section: Discussionmentioning

confidence: 99%

The antimicrobial activity of the alcohols fromMusca domestica

Gołębiowski

Dawgul

Kamysz

et al. 2012

Journal of Experimental Biology

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“…Operating conditions for the GC-MS analysis for lipid identification and methods for using authentic standards for determination of lipid quantities have been previously described (Buckner et al, 1999;Buckner and Hagen, 2003). Fatty acid pyrrolidides were analyzed using a Hewlett-Packard 5970 GC-MS equipped with a Supelco SP-2380 capillary column (30 m × 0.25 mm × 0.2 µm film thickness) and splitless injector at 260°C.…”

Section: Gas Chromatography-mass Spectrometrymentioning

confidence: 99%

Characterization of triacylglycerols from overwintering prepupae of the alfalfa pollinator Megachile rotundata (Hymenoptera: Megachilidae)

Buckner¹,

Kemp²,

Bosch³

2004

Arch Insect Biochem Physiol

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Alfalfa leafcutting bees, Megachile rotundata (F.), overwinter as prepupae. The internal lipids were extracted from prepupae that had been wintered at 4°C for 7 months. Megachile rotundata prepupae possessed copious quantities of internal lipids (20% of the fresh weight) that were extracted with CHCl 3 /methanol (2:1). Transmission electron microscopy revealed that lipids were stored within very large intracellular vacuoles. Separation by silica chromatography revealed that 88% of the internal lipids were triacylglycerols. Ester derivatives of fatty acids from triacylglycerol components were analyzed by gas chromatography-mass spectrometry and 15 fatty acid constituents were identified. The majority (76%) of the triacylglycerol fatty acids were unsaturated fatty acids. The major triacylglycerol fatty acid constituent (30%) was the C 16 monounsaturated fatty acid, palmitoleic acid (16:1, hexadec-9-enoic acid), with substantial amounts of linolenic acid (18:3, octadec-9,12,15-trienoic acid, 15%), palmitic acid (16:0, hexadecanoic acid, 14%) and oleic acid (18:1, octadec-9-enoic acid, 13%). Palmitoleic acid as the major fatty acid of an insect is an unusual occurrence as well as the presence of the 16-carbon polyunsaturated fatty acids, 16:2 and 16:3. The major intact triacylglycerol components were separated and identified by high performance liquid chromatography-mass spectrometry. A complex mixture of approximately 40 triacylglycerol components were identified and major components included palmitoyl palmitoleoyl oleoyl glycerol, palmitoyl palmitoleoyl palmitoleoyl glycerol, myristoyl palmitoleoyl palmitoleoyl glycerol, myristoleoyl palmitoyl palmitoleoyl glycerol, and palmitoyl palmitoleoyl linolenoyl glycerol. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. U.S. Department of Agriculture, Agricultural Research Service, Northern Plains Area, is an equal opportunity/ affirmative action employer and all agency services are available without discrimination.Abbreviations used: BSA = N,O-bis-(trimethylsilyl) acetamide; FAME = fatty acid methyl ester; L = linoleic acid; La = lauric acid; Ln = linolenic acid; M = myristic acid; Mo = myristoleic acid; O = oleic acid; P = palmitic acid; Po = palmitoleic acid; P L = palmitlinoleic acid; P Ln = palmitlinolenic acid; S = stearic acid; TAG = triacylglycerol; t-BME = tert-butylmethylether; TMS = trimethylsilyl.

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“…Alcohols ranging from C 24:0 to C 34:0 constituted the major lipid fraction of the cuticular waxes from Heliothis virescens pupae (Buckner et al, 1996). Wax esters consisting of even-numbered-carbon compounds from C 38 to C 64 were the major compounds (86%) present in the cuticle of Bemisia argentifolii nymphs and exuviae (Buckner et al, 1999). Cuticular waxes of insects also contain aldehydes as minor constituents.…”

Section: Chemical Composition Of Insect Cuticular Waxesmentioning

confidence: 99%

“…Cuticular waxes of insects also contain aldehydes as minor constituents. This group of waxes were identified in Heliothis virescens pupae, Helicoverpa zea pupae (Buckner et al, 1996), Aleurodes singularis adults and exuviae (Nelson et al, 1998), Bemisia argentifolii nymphs and exuviae (Buckner et al, 1999), Bemisia tabaci and Trialeurodes vaporariorum adults . Triacylglycerols are compounds of cuticular waxes but they could also be contaminants derived from body lipids.…”

Section: Chemical Composition Of Insect Cuticular Waxesmentioning

confidence: 99%

“…The initial temperature was significantly lower for the analysis of cuticular hydrocarbons, aldehydes, alcohols and wax esters in Bemisia argentifolii (50 °C to 320 °C) (Buckner et al, 1999), the fatty acids in Dendrolimus pini (50 °C to 310 °C) (Gołębiowski et al, 2010) and the surface waxes in Acanthoscelides obtectus (30 °C to 320 °C) (Gołębiowski et al, 2008b). The initial temperature depends on the molecular weights of the compounds to be analysed.…”

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confidence: 99%

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