The CTX-M β-lactamase pandemic

Cantón, Rafael; Coque, Teresa M.

doi:10.1016/j.mib.2006.08.011

Cited by 1,042 publications

(900 citation statements)

References 58 publications

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“…22 CTX-M-2, CTX-M-8 and CTX-M-9 groups were the most frequently detected CTX-M-type enzymes among ESBL-producing Enterobacteriaceae isolates from South American countries. 4,9,23 In the present study, CTX-M 2 (61%) was the predominant cluster. CTX-M-2 was first characterized and appears to be dominant in Argentina.…”

Section: Resultssupporting

confidence: 56%

“…24,25 Dissemination of CTX-M-9 and CTX-M-8 clusters has also been reported in Brazil. 4,26,27 Many of the CTX-M positive isolates harbored other β-lactam resistance enzymes and the association of types TEM, SHV and CTX-M (n = 20) was more frequent. Previous studies have shown that ESBL mediating plasmids may carry more than one β-lactamase gene and that they may be responsible for high-level β-lactamase resistance phenotypes.…”

Section: Resultsmentioning

confidence: 99%

“…3 In particular, CTX-M-type ESBLs have become the prevailing non-TEM, non-SHV ESBL among Enterobacteriaceae and is recognized as a rapidly growing family of ESBLs that preferentially hydrolyze cefotaxime rather than ceftazidime. 2,4,5 In recent studies, high rates of CTX-M enzymes among ESBL-producing K. pneu-pulmonary secretion (n = 5), catheter (n = 5), and other sites (n = 25). Preliminary identification of the isolates was accomplished using the Vitek® (bioMérieux) automated system and established biochemical procedures.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Chagas¹,

Alves²,

Vallim³

et al. 2011

Braz J Infect Dis

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Objective: The present study was undertaken to characterize CTX-M ESBL-producing Klebsiella pneumoniae collected from hospitals in different cities of Brazil. Material and Methods: Eighty-five K. pneumoniae strains isolated from hospitalized patients in six different hospitals of three cities of Brazil were analyzed. ESBL production was confirmed by the standard double-disk synergy test and the Etest®. The MIC 50 and MIC 90 for ESBL-producing isolates were determined by the Etest® method. The antimicrobial susceptibilities of bacterial isolates were determined using the agar diffusion method according to the CLSI. Screening for bla TEM, bla SHV, bla CTX-M genes and class 1 integron was performed by PCR amplification. To determine the genomic diversity of CTX-M-producers, isolates were analyzed by macrorestriction profile analysis following PFGE. Results and Discussion: Seventy-one K. pneumoniae isolates were ESBL-producing. PCR and sequencing experiments detected 38 CTX-M-producing K. pneumoniae belonged to groups CTX-M 1, CTX-M 2, CTX-M 8 and CTX-M 9. The association of different types ESBL (CTX-M, SHV and TEM) was frequent. All K. pneumoniae isolates carried class 1 integron. PFGE analysis revealed thirty-one clonal types among CTX-M-producing isolates. The data presented herein illustrate the diversity of genotypes of CTX-M producing K. pneumoniae among Brazilians hospitals.

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Section: Resultssupporting

confidence: 56%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Chagas¹,

Alves²,

Vallim³

et al. 2011

Braz J Infect Dis

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“…34 Although CTX-M group enzymes emerged later compared with TEM and SHV types, they have spread worldwide and specific subgroups have been localized in different geographic regions. 35,36 Among them, CTX-M-9 group β-lactamases (CTX-M-14 subtype) are the most widespread in China followed by CTX-M-1 group (CTX-M-3). [36][37][38] The occurrence of these enzymes vary from country to country, with Japan having the lowest bla gene prevalence rates among the neighboring countries.…”

Section: Discussionmentioning

confidence: 99%

Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains

et al. 2015

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Resistance to β-lactam antibiotics through β-lactamase production by Enterobacteriaceae continues to burden the health-care sector worldwide. Traditional methods for detection of β-lactamases are time-consuming and labor-intensive and newer methods with varying capabilities continue to be developed. The objective of this study was to develop a multiplex PCR (M-PCR) system for the detection of bla SHV , bla TEM , bla CTX-M-1 , bla CTX-M-9 and bla OXA-1 group genes and to apply it in clinical Klebsiella pneumoniae and Escherichia coli strains. To do this, we used group-specific PCR primers in singleplex reactions followed by optimization into multiplex reactions. Specificity and sensitivity of the M-PCR were then evaluated using 58 reference strains before its application to detect bla group genes in 203 clinical Enterobacteriaceae strains. PCR amplicons were sequenced to determine the β-lactamase subtypes. The M-PCR system exhibited 100% specificity and sensitivity. In all, 83.7% of K. pneumoniae and 89.8% of E. coli clinical strains harbored bla group genes with 46.9%, 40.1%, 15.0%, 21.1% and 6.1% of K. pneumoniae having bla SHV , bla TEM , bla CTX-M-1 , bla CTX-M-9 and bla OXA-1 group genes, respectively, whereas 12.2%, 77.6%, 22.4%, 36.7% and 8.2% of E. coli had bla SHV , bla TEM , bla CTX-M-1 , bla CTX-M-9 and bla OXA-1 group genes, respectively. Bla SHV-1 , bla SHV-11 , bla SHV-27 , bla SHV-33 , bla SHV-144 , bla TEM-1 , bla TEM-135 , bla OXA-1 , bla CTX-M-3 , bla CTX-M-9 , bla CTX-M-14 , bla CTX-M-15 , bla CTX-M-27 , bla CTX-M-55 , bla CTX-M-65 and bla CTX-M-104 were detected. In conclusion, the M-PCR system was efficient and versatile with an advantage of simultaneously detecting all the targeted bla group genes. Hence, it is a potential candidate for developing M-PCR kits for the screening of these genes for clinical or epidemiological purposes.

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“…The association of PMQR genes with extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases is note worthy (Canton & Coque 2006). Although a few studies describing PMQR determinants in selected isolates have been performed, these associations have not been previously studied in Argentina (Quiroga et al 2007, Jacoby et al 2009, Andres et al 2013).…”

mentioning

confidence: 99%

Prevalence of plasmid-mediated quinolone resistance determinants among oxyiminocephalosporin-resistant Enterobacteriaceae in Argentina

Cruz

Radice

Sennati

et al. 2013

Mem. Inst. Oswaldo Cruz

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High quinolone resistance rates were observed among oxyiminocephalosporin-resistant enterobacteria. In the present study, we searched for the prevalence of plasmid-mediated quinolone resistance (PMQR) genes within the 55 oxyiminocephalosporin-resistant enterobacteria collected in a previous survey. The main PMQR determinants were aac(6')-Ib-cr and qnrB, which had prevalence rates of 42.4% and 33.3%, respectively. The aac(6')-Ib-cr gene was more frequently found in CTX-M-15-producing isolates, while qnrB was homogeneously distributed among all CTX-M producers.

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The CTX-M β-lactamase pandemic

Cited by 1,042 publications

References 58 publications

Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Diversity of genotypes in CTX-M-producing Klebsiella pneumoniae isolated in different hospitals in Brazil

Development of a multiplex PCR system and its application in detection of blaSHV, blaTEM, blaCTX-M-1, blaCTX-M-9 and blaOXA-1 group genes in clinical Klebsiella pneumoniae and Escherichia coli strains

Prevalence of plasmid-mediated quinolone resistance determinants among oxyiminocephalosporin-resistant Enterobacteriaceae in Argentina

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