The Cytotoxic Effect of Nasodren Nasal Spray (Cyclamen Europaeum Extract) in L929 Fibroblastic Cell Culture

Abstract

A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : Na sod ren na sal spray (Cycla men eu ro pa e um ex tract), is used in so me coun tri es as an al ter na ti ve the ra pe u tic agent for si nu si tis. The aim of this study was to ives ti ga te the pos sib le cyto to xic ef fects of the drug on cell morp ho logy and vi a bi lity in L929 mo u se fibrob last cell cul tu re mo del. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : Na sod ren so lu ti on was di lu ted to the dilutions of 1:25, 1:50, 1:100, 1:200, and 1:400 (w/v), and these dilutions were gro u ped from I to V, res pec ti vely. The con trol gro up was pre pa red by using sa li ne in a 1:1 ra ti o. L929 fib rob las tic cell cul tu re ma te ri al was in cu ba ted in 96-cell in cu ba ti on me di um. Fol lo wing a 12-ho ur in cu ba ti on pe ri od, the ma te ri al was ta ken in to fresh cul tu re me di um and was sto red for five days af ter tre at ment with va ri o us di lu ti ons of the test ma te ri al. Cell morp ho logy was ob ser ved under mic ros co pe on 1 st , 2 nd , 3 rd , 4 th , and 5 th days. Cell vi a bi lity was eva lu a ted by 3-(4.5-di methy lthi azol-2-yl)-2,5-dip heny ltet ra zo li um bro mi de co lo ri met ric as say met hod on the sa me days. R Re e s su ul lt ts s: : Cells ma in ta i ned nor mal fib rob las tic sha pe and morp ho logy in Gro up V and the con trol gro up du ring the in cu ba ti on pe ri od. Nor mal ro und ring sha pe was apparently lost in Gro ups I-IV. Cell vi a bi lity dec re a sed in Gro ups I-IV, whe re as cell vi a bi lity in cre a sed in Gro up V and the con trol gro up. C Co on nc c l lu u s si i o on n: : Re sults of this study in di ca te that hig her con cen tra ti ons of Cycla men eu ropa e um ha ve to xic ef fects on cell vi a bi lity in vit ro. The re is ne ed for in vi vo ani mal stu di es befo re sa fely pres cri bing this drug as the first li ne tre at ment of si nu si tis.K Ke ey y W Wo or rd ds s: : Si nu si tis; Cyclamen Europaeum Extract Ö ÖZ ZE ET T A Am ma aç ç: : Na sod ren na zal sprey, (Cycla men eu ro pa e um eks tre si) si nü zit te da vi sin de al ter na tif bir te da vi aja nı ola rak ba zı ül ke ler de kul la nıl mak ta dır. Bu ça lış ma nın ama cı, ila cın, hüc re mor folo ji si ve ya şa ya bi lir li ği üze rin de ki ola sı si to tok sik et ki le ri ni L929 fa re fib rob last hüc re kül tür mode lin de araş tır mak tır. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Na sod ren çö zel ti si 1:25, 1:50, 1:100, 1:200 ve 1:400 (w/v) kon san tras yon la rın da ha zır lan dı ve sı ra sıy la I' den V'e ka dar grup lan dı rıl dı. Kon trol gru bu ise 1:1 serum fiz yo lo jik kul la nı la rak ha zır lan dı. L929 fib rob las tik hüc re kül tür ma ter ya li 96-hüc re li sak lama va sa tın da tu tul du. Çe şit li di lüs yon lar da ki çö zel ti ler de 12 sa at lik bek le til me sü re sin den son ra il gi li ma ter yal ta ze kül tür va sa tı na alı nıp beş gün su re ile tu tul du. Hüc re bi çi mi bi rin ci gün den beşin ci gü ne ka dar her gün mik ros kop la göz len di. Hü...

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