The cytotoxic effect of titanium particles phagocytosed by osteoblasts

Pioletti, Dominique P.; Takei, Hiroshi; Kwon, Soon Yong; Wood, D. L.; Sung, ki-wol

doi:10.1002/(sici)1097-4636(19990905)46:3<399::aid-jbm13>3.0.co;2-b

Cited by 154 publications

(144 citation statements)

References 39 publications

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“…This result is in opposition to a previous study using Ti particles [17]. The number and the size distribution of particles for both studies were similar.…”

Section: Discussioncontrasting

confidence: 99%

“…The small CPC particles induced an important negative e!ect on osteoblast functions which strongly suggested that the phagocytosis process of CPC particles was involved. Recent "ndings that osteoblasts phagocytosed Ti particles [29] and osteoblasts treated with cytochalasin-D (agent that inhibits phagocytosis by affecting actin "lament assembly of the cytoskeleton) almost completely suppressed the negative e!ect of Ti particle on osteoblast viability [17] supports this hypothesis. As for the macrophages or multinucleated giant cells [19], this phagocytosis process could produce an accumulation of calcium in mitochondria and could eventually lead to lysis of mitochondria and cell death.…”

Section: Discussionmentioning

confidence: 82%

“…The dose-dependent response of osteoblasts to particles has already been noted for cobalt [28] or titanium particles [17], but was not well established for CPC particles. The shape of the particles (round for -TCP and with angular shapes for the others) was not a parameter that in#uenced the osteoblast functions (data not shown).…”

Section: Discussionmentioning

confidence: 94%

“…These grains were round in shape and did not exceed 12 m [14]. Orthopedic implant studies have highlighted that wear particles could induce an in#ammatory response [15,16] or could alter the osteoblast functions [17,18] if their size was smaller than 10 m. It will then be important to verify the CPC biocompatibility not only in a bulk form, but also in a particulate form of micrometer size.…”

Section: Introductionmentioning

confidence: 99%

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The effects of calcium phosphate cement particles on osteoblast functions

et al. 2000

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Calcium phosphate cements (CPC) are increasingly used in the orthopedic "eld. This kind of cement has potential applications in bone defect replacements, osteosynthetic screw reinforcements or drug delivery. In vivo studies have demonstrated a good osteointegration of CPC. However, it was also observed that the resorption of CPC could create particles. It is known from orthopedic implant studies that particles can be responsible for the peri-implant osteolysis. Biocompatibility assessment of CPC should then be performed with particles. In this study, we quanti"ed the functions of osteoblasts in the presence of -TCP, brushite and cement particles. Two particle sizes were prepared. The "rst one corresponded to the critical diameter range 1}10 m and the second one had a diameter larger than 10 m. We found that CPC particles could adversely a!ect the osteoblast functions. A decrease in viability, proliferation and production of extracellular matrix was measured. A dose e!ect was also observed. A ratio of 50 CPC particles per osteoblast could be considered as the maximum number of particles supported by an osteoblast. The smaller particles had stronger negative e!ects on osteoblast functions than the larger ones. Future CPC development should minimize the generation of particles smaller than 10 m.

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“…This result is in opposition to a previous study using Ti particles [17]. The number and the size distribution of particles for both studies were similar.…”

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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The effects of calcium phosphate cement particles on osteoblast functions

et al. 2000

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“…We recently showed that wear particles also influence osteoblast behaviour [6][7][8][9]. Release of metal ions by the implant and their effect on osteoblasts has been mostly neglected until recently [10].…”

Section: Introductionmentioning

confidence: 99%

Large-scale gene expression analysis of osteoblasts cultured on three different Ti–6Al–4V surface treatments

Browne

Gregson

et al. 2002

Biomaterials

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To improve implant biocompatibility, we developed a simple cost-effective thermal surface treatment allowing an increase in the oxide layer thickness of a titanium (Ti) alloy used in orthopaedic implants. The goal of this study was to test in vitro the reaction of osteoblasts to the developed surface treatment and to compare it to the osteoblast reaction to two other surface treatments currently used in the practice of implant surgery. Quantification of osteoblast gene expression on a large scale was used in this study. The kinetics of gene expression over 120 h was followed for 58 genes to quantify the effect of the developed surface treatment. Twenty eight genes were further selected to compare the effects of surface treatments on osteoblasts. Based on the genes studied, we could propose a general pathway for the cell reaction according to the surface treatments used: (1) metal ion release changes the time course of gene expression in the FAK pathway; (2) once the accumulation of metal ions released from the Ti surface exceeds a threshold value, cell growth is diminished and apoptosis may be activated; (3) PTK up-regulation is also induced by metal ion release; (4) the expression of Bcl-2 family and Bax may suggest that metal ions induce apoptosis. The developed treatment seems to increase the Ti-6Al-4V biocompatibility as highlighted by the lower impact of this treatment by the different pathways studied, on the lower inflammatory reaction that could be induced, as well as by the lower induced osteoblast apoptosis compared to the two other surface treatments. r

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Combined effect of titanium particles and TNF-? on the production of IL-6 by osteoblast-like cells

Takei

Pioletti

Kwon

et al. 2000

J. Biomed. Mater. Res.

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To clarify the role of tumor necrosis factor (TNF)-alpha on osteoblast functions in the presence of metal particles, two human osteoblast-like cell lines (MG-63 and SaOS-2) were cultured with TNF-alpha in the presence or absence of titanium particles in vitro. A combination of TNF-alpha and titanium particles showed additive effects on inhibition of cell proliferation and alkaline phosphatase production. On the other hand, production of interleukin-6, which is well known to induce osteoclastogenesis and to directly stimulate bone resorption, was additively stimulated by the combination of TNF-alpha and titanium particles. These results suggest that the association of TNF-alpha and titanium particles may play an important role in the pathogenesis of periprosthetic osteolysis through two different pathways: a reduced periprosthetic bone formation due to inhibition of osteoblast proliferation and alkaline phosphatase production, and osteoblast-mediated activation of osteoclastic bone resorption as suggested by the enhancement of interleukin-6 production.

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The cytotoxic effect of titanium particles phagocytosed by osteoblasts

Cited by 154 publications

References 39 publications

The effects of calcium phosphate cement particles on osteoblast functions

The effects of calcium phosphate cement particles on osteoblast functions

Large-scale gene expression analysis of osteoblasts cultured on three different Ti–6Al–4V surface treatments

Combined effect of titanium particles and TNF-? on the production of IL-6 by osteoblast-like cells

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