The Dad1 subunit of the yeast kinetochore Dam1 complex is an intrinsically disordered protein

Waldo, Jennifer Turner; Greagor, Stacy A.; Iqbal, Aruba J.; Gittens, Abiola S.A.; Grant, Kemone K.

doi:10.1016/j.bbrc.2010.08.050

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…First, we identified putative C. albicans homologs of Dam1 complex members (Figure 1A and Table S1). The C. albicans Dad1 homolog is an intrinsically disordered protein with a structure similar to that of S. cerevisiae Dad1 [11]. By chromatin immunoprecipitation (ChIP), Dad1-GFP and Dam1-GFP bind centromere DNA regions that co-localize with regions bound by CENP-A (Figure 1B).…”

Section: Resultsmentioning

confidence: 99%

The Requirement for the Dam1 Complex Is Dependent upon the Number of Kinetochore Proteins and Microtubules

2011

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Summary The Dam1 complex attaches the kinetochore to spindle microtubules and is a processivity factor in vitro [1, 2]. In Saccharomyces cerevisiae, which has point centromeres that attach to a single microtubule, deletion of any Dam1 complex member results in chromosome segregation failures and cell death [3–5]. In Schizosaccharomyces pombe, which has epigenetically-defined regional centromeres that each attach to 3–5 kinetochore microtubules, Dam1 complex homologs are not essential [6]. To ask why the complex is essential in some organisms and not in others, we used Candida albicans, a multimorphic yeast with regional centromeres that attach to a single microtubule [7]. Interestingly, the Dam1 complex was essential in C. albicans, suggesting that the number of microtubules per centromere is critical for its requirement. Importantly, by increasing CENP-A expression levels, more kinetochore proteins and microtubules were recruited to the centromeres, which remained fully functional. Furthermore, Dam1 complex members became less crucial for growth in cells with extra kinetochore proteins and microtubules. Thus, the requirement for the Dam1 complex is not due to the DNA-specific nature of point centromeres. Rather, the Dam1 complex is less critical when chromosomes have multiple kinetochore complexes and microtubules per centromere, implying that it functions as a processivity factor in vivo as well as in vitro.

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Section: Resultsmentioning

confidence: 99%

The Requirement for the Dam1 Complex Is Dependent upon the Number of Kinetochore Proteins and Microtubules

2011

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“…We have been examining the individual subunits of the Dam1 complex from Candida albicans, as some of these proteins are capable of being expressed in bacteria. For example, the C. albicans Dad1p has been shown to behave as an intrinsically disordered protein when isolated (J. T. Waldo, Greagor, Iqbal, Gittens, & Grant, 2010). Based on these studies, a working model is that Dad1p undergoes a structural transition upon binding to the other components of the complex.…”

Section: Introductionmentioning

confidence: 99%

Divalent Cations Affect the Stability and Structure of Dad2p, a Subunit of the Candida Albicans Kinetochore Dam1 Complex

Waldo¹,

Dolma²,

Rouse³

2016

JMBR

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The heterodecameric Dam1 complex is involved in establishing and maintaining the connection between the kinetochore and the mitotic spindle during mitosis. Biochemical studies of the reconstituted complex have shed light upon how it interacts with microtubules. However, little information about the biochemical properties of the isolated subunits has been available. This report examines the stability and structure of Dad2p, one of the Dam1 complex subunits isolated from Candida albicans. By employing differential scanning fluorimetry, protease protection and hydrodynamic analyses, we show that Dad2p is specifically responsive to the presence of divalent cations. This observation may be important for understanding the dynamic structure and regulation of the Dam1 complex in fungal cells.

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Chromosome Components Important for Genome Stability in Candida albicans and Related Species

Sreekumar

Varshney

Sanyal

2017

Candida Albicans: Cellular and Molecular Biology

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The Dad1 subunit of the yeast kinetochore Dam1 complex is an intrinsically disordered protein

Cited by 4 publications

References 25 publications

The Requirement for the Dam1 Complex Is Dependent upon the Number of Kinetochore Proteins and Microtubules

The Requirement for the Dam1 Complex Is Dependent upon the Number of Kinetochore Proteins and Microtubules

Divalent Cations Affect the Stability and Structure of Dad2p, a Subunit of the Candida Albicans Kinetochore Dam1 Complex

Chromosome Components Important for Genome Stability in Candida albicans and Related Species

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