The degree of deacetylation of chitosan: optimization of the IR method

Chitin/chitosan oligosaccharides composed of 2-acetamido-2-deoxy-D-glucopyranose (GlcNAc) and/or 2-amino-2-deoxy-D-glucopyranose (GlcN) were prepared by chemical degradation of chitin or chitosan and separated by gel permeation chromatography. Oligosaccharides obtained after enzymatic hydrolysis of chitosan [F(A) 0.19] with a fungal chitinase were derivatized by reductive amination with 2-aminoacridone and sequenced by matrix-assisted laser desorption ionization time-of-flight postsource decay (PSD) mass spectrometry (MS). The sequence of a trimer, D1A2, was established as D-A-A. The composition of a hexamer D3A3 was ca. 65% D-A-D-D-A-A and 35% D-D-A-D-A-A. The PSD MS of a nonamer D5A4-amac revealed four isobaric species D-X-Y-D-X-Y-D-A-A, where A is GlcNAc, D is GlcN, and X and Y (X not equal Y) are mutually either D or A. This structure motif was also observed in a dodecamer D7A5 which was composed of eight isobaric sequences of the general formula (D-X-Y)(3)-D-A-A.

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“…F A values of chitin and chitosan were determined by potentiometric titration 22 or by FT-IR spectroscopy .…”

Section: Methodsmentioning

confidence: 99%

“…F A values of chitin and chitosan were determined by potentiometric titration 22 or by FT-IR spectroscopy. 23 M v was estimated by viscosimetry. 24 Chitosan [F A 0.48] from krill, Euphausia superba, was a gift of fcb (Bremerhaven, Germany).…”

Section: Introductionmentioning

confidence: 99%

Sequence Analysis of Chitooligosaccharides by Matrix-Assisted Laser Desorption Ionization Postsource Decay Mass Spectrometry

et al. 2002

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“…1 It has been estimated that chitin is synthesized in nature at a level of up to 1-10 billion tonnes per year. 2 The major source of chitin is crustacean shells, which are a by-product of the seafood refining industry, although chitin is also found in the skeletons of several insects and in the cell walls of several fungi. 3 Chitosan is partially deacetylated chitin and is mainly produced from chitin by hydrolysis of the amide C-N bond by strong alkali.…”

Section: Introductionmentioning

confidence: 99%

Comparison of chitosans with different molecular weights as possible wood preservatives

Eikenes¹,

Alfredsen²,

Christensen

et al. 2005

J Wood Sci

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Fungi cause serious problems in wood utilization, and environmentally benign wood protection is required as an alternative to traditional chemicals. Chitosan has shown promising antimicrobial properties against several microorganisms. In this study, we present the characterization of and antifungal properties of a commercial chitosan formulation developed for impregnation of wood. A broad range of chemical and mycological methods were used to evaluate the uptake, fixation, and antifungal properties of chitosan for wood preservation. The results show that the higher the uptake of chitosan the lower the relative recovery of chitosan in wood after leaching, and the higher the molecular weight of chitosan the higher the recovery. Chitosan with high molecular weight proved to be more efficient against decay fungi than chitosan with low molecular weight. The fungi tested on chitosan-amended nutrient agar medium were totally inhibited at 1% (w/v) concentration. In decay studies using small wood blocks, 4.8% (w/v) chitosan concentration gave the best protection against brown rot fungi.

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“…‐ the absorption bands at λ = 896 cm −1 corresponding to stretching glycoside linkage that are characteristic bands for chitosan macromolecules and have been also used as reference bands. 20–23…”

Section: Resultsmentioning

confidence: 99%

Estimation of the performance stability of the newly developed topical haemostatic agents based on the chitosan/alginate fibrids

Struszczyk

Wilbik-Hałgas

Miklas

et al. 2016

Textile Research Journal

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Designing medical devices requires a wide range of verification steps for estimation of the performance and safety. Designing the research program needs a rational selection of appropriate testing methods (in preclinical and clinical studies) for determination of the risk of potential incompatibilities resulting in adverse events. The significance of the appropriate selection of the testing method is increased in advanced medical devices. The presented research considers the verification of the functional properties of recently developed topical haemostatic agents with the use of the chitosan/alginate fibrids, based on the previously elaborated risk analysis made according to the guidelines of the PN-EN-ISO 14971:2012 and PN-EN ISO 22442-1:2008 standards. The aim of this research was to verify the stability of the performance of the newly developed haemostatic agents during storage. The defined aim of the study arose from the thesis that the appropriate selection of raw materials and a new manner of reprocessing them enabled keeping the usability of the final product for at least two years.

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The degree of deacetylation of chitosan: optimization of the IR method

Cited by 11 publications

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Sequence Analysis of Chitooligosaccharides by Matrix-Assisted Laser Desorption Ionization Postsource Decay Mass Spectrometry

Sequence Analysis of Chitooligosaccharides by Matrix-Assisted Laser Desorption Ionization Postsource Decay Mass Spectrometry

Comparison of chitosans with different molecular weights as possible wood preservatives

Estimation of the performance stability of the newly developed topical haemostatic agents based on the chitosan/alginate fibrids

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