The development of a novel serotyping-NS1-ELISA to identify serotypes of dengue virus

Puttikhunt, Chunya; Prommool, Tanapan; U-thainual, Nathaporn; Ong-ajchaowlerd, Prapapun; Yoosook, Kroong; Tawilert, Chutithorn; Duangchinda, Thaneeya; Jairangsri, Aroonroong; Tangthawornchaikul, Nattaya; Malasit, Prida; Kasinrerk, Watchara

doi:10.1016/j.jcv.2011.01.001

Cited by 37 publications

(44 citation statements)

References 28 publications

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“…In this regard, a new NS1 detection kit that differentiates DENV serotypes and that has excellent sensitivity and specificity has been developed using serotype-specific anti-NS1 monoclonal antibodies. 38,39 In future studies, we will determine the diagnostic efficacy of the Platelia Dengue NS1 Ag kit for surveillance for DENV in vector populations in dengue endemic areas and will address the issues of detection of the NS1 antigen in field-collected mosquitoes infected with different DENV serotypes.…”

Section: Discussionmentioning

confidence: 99%

Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Voge¹,

Sánchez-Vargas²,

Blair³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. Epidemic dengue has emerged throughout the tropical world. In the continued absence of a vaccine against dengue virus (DENV), mosquito vector surveillance and control programs are essential to reduce human infections. An effective test to detect DENV in infected mosquitoes would be a valuable addition to the surveillance effort. We investigated DENV detection in infected Aedes aegypti using a commercially available DENV non-structural protein 1 (NS1) ELISA kit (Platelia Dengue NS1 Ag), and by reverse transcription-polymerase chain reaction (RT-PCR) and virus isolation assays. The DENV-infected mosquitoes were subjected to field-relevant conditions and assayed individually and pooled with uninfected mosquitoes. Overall, DENV NS1 antigen was detected in 98% of infected mosquitoes/ pools versus 79% for RT-PCR and 29% for virus isolation. Our results indicate that NS1 is an excellent analyte for detection of DENV in Ae. aegypti and that the tested NS1 antigen kit provides a sensitive, rapid, and convenient test for DENV surveillance in mosquitoes.

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Section: Discussionmentioning

confidence: 99%

Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Voge¹,

Sánchez-Vargas²,

Blair³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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“…Although highly conserved, serotypespecific NS1 monoclonal antibodies have been raised and applied for NS1-based dengue serotype identification assays [105][106][107][108][109]. A study by Puttikhunt et al has shown an overall sensitivity of 76.5% and specificity of 100% for diagnosis of dengue while having serotyping sensitivities of 100% for DENV 1, 3 and 4 and 82.4% for DENV2 [109]. However, the sensitivity of these tests may differ with different strains of DENV as the magnitude of NS1 secretion appears to be strain dependent [110].…”

Section: Antigen Detectionmentioning

confidence: 99%

Diagnosis of dengue: an update

Tang

Ooi

2012

Expert Review of Anti-infective Therapy

113

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“…The reaction was stopped by adding 50 ml of 2 N sulfuric acid. The plate was measured for the absorbance at 450/620 nm on a microplate reader (Anthos 2010, Salzburg, Austria) (12,13).…”

Section: Methodsmentioning

confidence: 99%

Synergistic Effect of TNF-α and Dengue Virus Infection on Adhesion Molecule Reorganization in Human Endothelial Cells

2017

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SUMMARY: Dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) is a severe pathological manifestation of dengue virus (DENV) infection. Enhanced production of cytokines in dengue patients is proposed to induce endothelial barrier instability resulting in increased vascular leakage. Tumor necrosis factor (TNF)-a is an inflammatory cytokine that activates endothelial cells and enhances vascular permeability and plasma leakage in DHF/DSS. The present study investigated the in vitro effect of TNF-a and DENV infection on the expression of adherence junction proteins, tight junction proteins, and membrane integrity of human endothelial cell lines. Immunofluorescence staining and western blot analysis demonstrated platelet endothelial cell adhesion molecule-1 (PECAM-1) reorganization and decreased levels of the tight junction protein occludin in human endothelial cells treated with TNF-a and DENV, compared to mock, DENV, or TNF-a-treated cells. Permeability assessed by FITC-dextran as a transport molecule was increased and correlated with the unusual reorganization of PECAM-1. The altered distribution of PECAM-1 and low occludin protein levels in human endothelial cells treated with TNF-a and DENV correlated with increased permeability. In conclusion, the synergistic effect of TNF-a and DENV induced permeability changes in endothelial cells. These results contribute to the understanding of the mechanisms underlying enhanced vascular permeability in DENV infection.

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The development of a novel serotyping-NS1-ELISA to identify serotypes of dengue virus

Cited by 37 publications

References 28 publications

Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Diagnosis of dengue: an update

Synergistic Effect of TNF-α and Dengue Virus Infection on Adhesion Molecule Reorganization in Human Endothelial Cells

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The development of a novel serotyping-NS1-ELISA to identify serotypes of dengue virus

Cited by 37 publications

References 28 publications

Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Detection of Dengue Virus NS1 Antigen in Infected Aedes aegypti Using a Commercially Available Kit

Diagnosis of dengue: an update

Synergistic Effect of TNF-&alpha; and Dengue Virus Infection on Adhesion Molecule Reorganization in Human Endothelial Cells

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Product

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Synergistic Effect of TNF-α and Dengue Virus Infection on Adhesion Molecule Reorganization in Human Endothelial Cells