1991
DOI: 10.1016/0378-1135(91)90118-y
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The development of an international reference panel of monoclonal antibodies for the differentiation of hog cholera virus from other pestiviruses

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Cited by 128 publications
(64 citation statements)
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“…Porcine kidney cells such as PK 15 cell line remain the most suitable system for its propagation. Due to the lack of cytophatic effect, accurate titration of the virus infectivity was based on the identification of microscopic fluorescent foci or by PLA (Jensen 1981, Edwards et al 1991, Dahle et al 1993, Freitas 1993). …”
Section: Resultsmentioning
confidence: 99%
“…Porcine kidney cells such as PK 15 cell line remain the most suitable system for its propagation. Due to the lack of cytophatic effect, accurate titration of the virus infectivity was based on the identification of microscopic fluorescent foci or by PLA (Jensen 1981, Edwards et al 1991, Dahle et al 1993, Freitas 1993). …”
Section: Resultsmentioning
confidence: 99%
“…Likewise, in vitro VN tests demonstrated that BVDV isolated from different clinical syndromes were antigenically related, suggesting that distinct serotypes did not exist (14,26). Thereafter, observations of antigenic relationships were extended to the other Pestiviruses -classical swine fever virus (CSFV) and border disease virus (BDV) (7,27). The concepts of antigenic similarity and of the existence of a single serologic type for BVDV persisted until recently, so that most BVDV vaccines produced up to the 90's were based on a single BVDV isolate.…”
Section: Discussionmentioning
confidence: 99%
“…The availability of cytopathic viruses greatly facilitated and improved the in vitro virus-neutralization studies (7,16). Nonetheless, more definitive and precise characterization of the antigenic variability was only possible with the development of BVDVmAbs in the late 1980's (22,27) and more recently, as more BVDV gene sequences became available (28)(29)(30).…”
Section: Discussionmentioning
confidence: 99%
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“…The cultures were fixed in 80% acetone and tested for CSFV antigen by indirect immunofluorescent antibody test. After acetone fixation, wells were stained with monoclonal mouse anti-CSFV antibody (WH303, Central Veterinary Laboratory, New Haw, Addlestone, UK) 8 and fluoroscein isothiocyanate (FITC)-conjugated anti-mouse immunoglobulin G and then viewed with a fluorescence microscope for evidence of specific viral antigen. Wells were also stained with immunohistochemistry.…”
mentioning
confidence: 99%