The development of attenuation compensation models of fluorescence spectroscopy signals

Dremin, Viktor; Zherebtsov, Evgeny; Rafailov, Ilya E.; Vinokurov, Andrey Y.; Novikova, Irina N.; Zherebtsova, Angelina I.; Litvinova, Karina S.; Dunaev, Andrey V.

doi:10.1117/12.2229451

Cited by 17 publications

(14 citation statements)

References 11 publications

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“…The use of additional optical methods (e.g., diffuse reflectance spectroscopy) can be considered. Extending the number of implemented modalities allows for obtaining the data, which can be used to compensate the influence of blood on recorded fluorescence spectra [22]. If the blood content of the living tissue is to be kept unaffected, then to eliminate the probe pressure, an embedded in the probe noncontact distance measurements with subsequent automation of the recording procedure at a fixed optimal distance can be one of the possible solutions.…”

Section: Resultsmentioning

confidence: 99%

Fiber-Optic System for Intraoperative Study of Abdominal Organs during Minimally Invasive Surgical Interventions

et al. 2019

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The paper presents the results of experimental measurements of endogenous fluorescence and blood perfusion in patients with pathology of the organs of hepatopancreatoduodenal area in vivo. A custom setup combining channels for fluorescence spectroscopy (excitation wavelengths of 365 nm and 450 nm) and laser Doppler flowmetry (1064 nm) with fibre optical probe for nondestructive laparoscopic measurements has been developed and applied during minimally invasive operation procedure. Preliminary measurements with two aforementioned channels have been performed at specified excitation wavelengths. The possibility of obtaining fluorescence spectra and laser Doppler flowmetry signals in vivo during minimally invasive interventions was shown. Obtained data show perspectives of further research on technical and methodological development of optical diagnostic methods for minimally invasive surgery. The obtained results can be used to provide a deeper understanding of pathological processes influence on optical properties of abdominal organs tissues, which will ultimately help surgeons to determine the state of vitality in tissues and mucous membranes directly during the process of surgical intervention.

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Section: Resultsmentioning

confidence: 99%

Fiber-Optic System for Intraoperative Study of Abdominal Organs during Minimally Invasive Surgical Interventions

et al. 2019

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“…This method is based on probing the biological tissue with laser radiation and analyzing the Doppler shift caused by light scattered and reflected by erythrocytes moving in microcirculatory bed. LDF was used for assessing blood flow parameters 14 of tissues in addition to FS, as it is widely known that blood affects recorded spectra 15 .…”

Section: Methodsmentioning

confidence: 99%

Optical diagnostics of bile duct tissues state with tumor compression

Kandurova

Dremin²,

Zherebtsov

et al. 2019

Saratov Fall Meeting 2018: Optical and Nano-Technologies for Biology and Medicine

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The paper presents the results of in vivo experimental measurements of the endogenous fluorescence and blood perfusion in common bile duct tissues in obstructive jaundice patients during minimally invasive surgical operations. Nowadays the type of interventions become more widespread for treating hepatopancreatobiliary pathologies. An effective feedback which can be established by one or more optical diagnostic channels integrated into the standard instruments for minimally invasive manipulations would be of particular value to practical surgeons. To collect reference data, an experimental study has been conducted using a fiber optical probe with fluorescence spectroscopy and laser Doppler flowmetry channels. For every measurement, the areas of interest were different areas of common bile duct obstructed by a malignant tumor. Obtained results demonstrated significant variety registered intensities of fluorescence spectra and values of blood perfusion. Tested fiber optical endoscopic system showed sensitivity to the tissue state in the investigated area, so the two modal approach seems to be promising for further research and implementation in clinical practice.

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“…2 The attenuation and changes of the fluorescence signal due to the blood volume alterations hide the changes linked with the concentration of fluorescent agents effectively. 3 Moreover, the highly variable parameter directly affects the sampling depth and volume due to the absorption of the excitation radiation. At this time, several techniques have been developed to compensate for the influence of blood absorption on the fluorescence measurements in vivo.…”

Section: Introductionmentioning

confidence: 99%

Fibre-optic probe for fluorescence diagnostics with blood influence compensation

Zherebtsov

Dremin²,

Zharkikh³

et al. 2018

Dynamics and Fluctuations in Biomedical Photonics XV

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To minimise the influence of blood content on the fluorescence measurements in vivo, a fibre optical probe combining fluorescence and diffuse reflectance measurements was developed. For the inverse solution of the blood content recovery, a set of neural networks trained by the Monte Carlo generated learning set was used. An approach of fluorescence measurements triggered by simultaneous real-time measurements of blood content in living tissue during moderate changes in contact pressure of the optic probe is proposed. The method allows one to decrease the necessary pressure on the probe as well as increase the repeatability of the measurements. The developed approach was verified in a series of experiments on volunteers with fluorescence excitation at 365 nm and 450 nm. The proposed technology is of particular interest in the development of new fluorescence-based optical biopsy systems.

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The development of attenuation compensation models of fluorescence spectroscopy signals

Cited by 17 publications

References 11 publications

Fiber-Optic System for Intraoperative Study of Abdominal Organs during Minimally Invasive Surgical Interventions

Fiber-Optic System for Intraoperative Study of Abdominal Organs during Minimally Invasive Surgical Interventions

Optical diagnostics of bile duct tissues state with tumor compression

Fibre-optic probe for fluorescence diagnostics with blood influence compensation

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