The Diagnostic Importance of Circulating Microrna for Non-Alcoholic Fatty Liver Disease: Literature Review

Gimadiev, P P; Niiazov, A R; Mukhin, V.E.; Ogurtsov, Pavel

doi:10.18821/0869-2084-2019-64-12-723-729

Cited by 4 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MiRNAs as a noninvasive marker may show promise in the diagnosis of NAFLD by replacing liver biopsy and provide a new strategy for NAFLD [21,22]. The expression of miR-103-3p is related to steatosis activity, fibrosis score, stages, and prognostic markers of NAFLD [16]. We found that miR-103-3p was increased in the FFA-treated hepatocytes and liver tissues of the mice with NAFLD and may be a prospective biomarker for NAFLD diagnosis.…”

Section: Discussionmentioning

confidence: 77%

“…MiR-103-3p is an important member of the miRNA family and is related to the regulation of pathological processes such as osteoporosis, diabetes, and obesity [14,15]. Recent research has shown that miR-103-3p is a noninvasive prospective biomarker for NAFLD diagnosis [16]. The increase in miR-103-3p is related to liver steatosis, a type of NAFLD [17].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

MiR-103-3p promotes hepatic steatosis to aggravate nonalcoholic fatty liver disease by targeting of ACOX1

et al. 2022

View full text Add to dashboard Cite

Background Nonalcoholic fatty liver disease (NAFLD) is a major risk factor for hepatocellular carcinoma, and alterations in miRNA expression are related to the development of NAFLD. However, the role of miRNAs in regulating the development of NAFLD is still poorly understood. Methods We used qRT-PCR to detect the level of miR-103-3p in both cell and mouse models of NAFLD. Biochemical assays, DCF-DA assays, Oil red O staining and HE staining were used to detect the role of miR-103-3p in NAFLD development. Target genes of miR-103-3p were predicted using the TargetScan database and verified by qRT-PCR, western blot and dual-luciferase assays. Results The expression of miR-103-3p increased in both NAFLD model cells and liver tissues from the NAFLD mouse model. Inhibition of miR-103-3p significantly alleviated the accumulation of lipid droplets in free fatty acid-treated L02 cells and liver tissues from mice with NAFLD. Inhibition of miR-103-3p reduced the contents of H2O2, TG, ALT, and AST and ROS production while increasing the ATP content. Moreover, the miR-103-3p antagomir alleviated liver tissue lesions in mice with NAFLD. Further studies identified ACOX1, a key enzyme for the oxidation and decomposition of fatty acids, as a direct target of miR-103-3p. Conclusions These findings identified a negative regulatory mechanism between ACOX1 and miR-103-3p that promotes the pathogenesis of NAFLD and suggested that inhibition of miR-103-3p may be a potential treatment strategy for NAFLD.

show abstract

Section: Discussionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

MiR-103-3p promotes hepatic steatosis to aggravate nonalcoholic fatty liver disease by targeting of ACOX1

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Next, we studied whether the expression of miR-21 associated with blood glucose concentration in the GDM rat model. Considering that STZ in rats can produce severe diabetes (blood glucose exceeding 16 mmol/l) [22][23][24] or mild diabetes (blood glucose of 7-16 mmol/l), 25,26 STZ (40-50 mg/kg body weight) given intravenously or intraperitoneally in rats can induce severe diabetes. 27 According to the literature and our preliminary results, we selected 3560 mg/kg STZ to induce moderate and severe GDM, respectively.…”

Section: Discussionmentioning

confidence: 99%

<p>Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration</p>

Guan¹,

Shen²,

Cao³

et al. 2020

DMSO

View full text Add to dashboard Cite

This study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients. Materials and Methods: qRT-PCR was used to detect the differential expression of miR-21 in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene PPAR-α of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of PPAR-α on cell function. Results: miR-21 is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, miR-21-5p knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR-α expression can restore miR-21 knockdownmediated cell growth and metastasis inhibition. Additionally, decreased expression of miR-21 but increased expression of-PPAR-α was observed in patients with GDM and GDM rats. Conclusion: The expression of the placental miR-21-5p, which inhibits cell growth and infiltration by up-regulating PPAR-α, is downregulated in pregnant GDM patients, which in turn may affect the placental function.

show abstract

Acyl CoA oxidase: from its expression, structure, folding, and import to its role in human health and disease

Kashyap,

Deb,

Battineni

et al. 2023

Mol Genet Genomics

View full text Add to dashboard Cite

The Diagnostic Importance of Circulating Microrna for Non-Alcoholic Fatty Liver Disease: Literature Review

Cited by 4 publications

References 20 publications

MiR-103-3p promotes hepatic steatosis to aggravate nonalcoholic fatty liver disease by targeting of ACOX1

MiR-103-3p promotes hepatic steatosis to aggravate nonalcoholic fatty liver disease by targeting of ACOX1

<p>Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration</p>

Acyl CoA oxidase: from its expression, structure, folding, and import to its role in human health and disease

Contact Info

Product

Resources

About