The differential fluorescence of bacteria stained with acridine orange and the effects of heat

Back, J.P.; Kroll, R.G.

doi:10.1111/j.1365-2672.1991.tb04481.x

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Cited by 25 publications

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“…Furthermore, there was a risk of spoiling samples if the instrument was not sufficiently flushed with sheath fluid following the use of NaOCl. Finally, inconsistencies in AO staining of active and inactive bacteria have been reported[23]. Thus, characterisation of AO fluorescence is needed when applying the stain to a different biological system.…”

Section: Discussionmentioning

confidence: 99%

Acridine orange as an indicator of bacterial susceptibility to gentamicin

Mason

Lloyd

2006

FEMS Microbiology Letters

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We have studied the response of Escherichia coli NCTC10418 to gentamicin with flow cytometry. The susceptibility of individual bacterial cells to the antibiotic was assessed by differential staining using the metachromatic dye, acridine orange. Exponential phase cultures were exposed to the minimum bactericidal concentration of gentamicin and analysed at regular intervals over 90 min. Within 60 min of exposure to the drug, two sub-populations of organisms could be distinguished in cultures by their different acridine orange-associated fluorescence emissions of < 550 nm and > 550 nm. The number of bacteria exhibiting acridine orange-associated fluorescence at > 550 nm corresponded to counts of colony forming units.

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Section: Discussionmentioning

confidence: 99%