The Differentiation of Hemolytic Streptococci from Various Sources by the Group Precipitin Reaction and by Biochemical Tests

Abstract

This study was undertaken, first, to determine the prevalence of the different serologic groups of hemolytic streptococci in specimens collected in this vicinity; and, second, to obtain additional data on the correlation between certain biochemical tests and the group precipitin reaction of Lancefield (1933). A total of 561 cultures was studied of which 188 were isolated from material from human infections, 53 from normal persons, 8 from infected animals, and 312 from raw milk. Several such studies have been made since the Lancefield technic has been available. A similar survey of cultures from human and animal sources was reported by Coffey (1938). Butler (1938), Plummer (1935), Reid and Browne (1939) and Kobayashi (1939) have studied cultures derived chiefly from a variety of human sources. Among others who have classified hemolytic streptococci by the precipitin test are: Hare (1935), Davis and Guzdar (1936), Foote, Welch, West and Borman (1936), and Kodama, Ozaki, Nishiyama and Chiku (1938), who studied cultures from the nose and throat; Hare and Maxted (1935) and Smith and Sherman (1938), who examined human feces; Lancefield and Hare (1935), Rolfs, Trussell and Plass (1938), and Congdon (1935), who collected strains from the vagina and respiratory tract of parturient women; Colebrook, Maxted and Johns (1935), whose cultures were secured from human skin; and Plastridge and Hartsell (1937), Valentine (1938),

The Fermentation of Glycerol by Streptococci

“…6.8 6.7 4.3 & Keogh (1940) who studied 169 strains, and with the previous reports of Edwards (1932) and Sherman (1937). Gunnison, et al (1940a), however, did not observe glycerol fermentation with "human" group C strains under their test conditions.…”

Titration and Partial Characterization of a Soluble Hemolysin of a Group D Streptococcus

“…Hare and Maxted (1935) isolated from feces 13 Group D streptococci which did not possess a soluble hemolysin. Gunnison et al, (1940), who isolated 7 enterococci from milk, feces, and urine, were not able to demonstrate a soluble hemolysin in whole cultures which was active against rabbit erythrocytes. Plummer (1941) found 1 Group D streptococcus that produced a soluble hemolysin in 10 per cent serum-beef infusion broth but 3 other Group D organisms failed to do so.…”