The dilution factor of intravenously injected hemopoietic stem cells

Matioli, G.T.; Vogel, Helmut; Niewisch, Helgard

doi:10.1002/jcp.1040720310

Cited by 28 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1974) are therefore two to three times the seeding level observed at 24 hr (e.g. 6-12%; Playfair & Cole, 1965;Matioli, Vogel & Niewisch, 1968;Kretchmar & Conover, 1969a;Lahiri & van Putten, 1969;Testa et af., 1972;Beran & Tribukait, 1974;Coggle & Gordon, 1975;Hasthorpe & Hodgson, 1977). These latter estimates may therefore more accurately reflect the 'true' stem cell seeding efficiency.…”

Section: Discussionmentioning

confidence: 91%

The Relationship Between Stem Cell Seeding Efficiency and Position in Cell Cycle

Monette

Demello

1979

Cell Proliferation

View full text Add to dashboard Cite

The seeding efficiency of colony‐forming cells from normal, regenerating and velocity‐sedimented cycling and non‐cycling narrow preparations was compared. Colony‐forming cells in cycle were found to exhibit a 50% reduction in splenic seeding when compared to normal marrow or sedimented non‐cycling cells. The results of this study indicate that the spleen colony assay underestimates the total number of colony‐forming cells by a fraction which is directly related to the number of cells in cycle.

show abstract

Section: Discussionmentioning

confidence: 91%

The Relationship Between Stem Cell Seeding Efficiency and Position in Cell Cycle

Monette

Demello

1979

Cell Proliferation

View full text Add to dashboard Cite

show abstract

“…In three experiments with the highest enrichment, an average of 10 + 1 day-12 spleen colonies was observed per 100 injected sorted cells. This gives a minimum value for the seeding efficiency (f factor) of sorted CFU-S in spleen of 0.1, or twice the value measured in classic retransplantation experiments (7,28,34). The latter method, in fact, measures the f factor for the second transplantation.…”

Section: Discussionmentioning

confidence: 96%

Isolation of murine pluripotent hemopoietic stem cells.

Visser¹,

Bauman²,

Mulder³

et al. 1984

The Journal of Experimental Medicine

302

124

View full text Add to dashboard Cite

A method described to purify pluripotent hemopoietic stem cells ( PHSC ) from adult mouse bone marrow. The method consists of three separation steps. First, bone marrow cells are centrifuged in a discontinuous metrizamide gradient and simultaneously labeled with wheat germ agglutinin-fluorescein isothiocyanate (WGA-FITC). Second, the low density cells are analyzed by a fluorescence-activated cell sorter (FACS) and the WGA-positive cells with medium forward and low perpendicular light scatter intensities are sorted. The WGA-FITC is removed from the cells by incubation with N-acetyl-D-glucosamine. Finally, the sorted cells are incubated with anti-H-2K-biotin and avidin-FITC and sorted a second time to enrich cells with high H-2K density. The sorted cells gave rise to 2 spleen colonies per 100 injected cells at 8 d and 6.6 colonies per 100 cells at 12 d after transplantation into lethally irradiated syngeneic recipients. The average enrichment factor for day 12 CFU-S (colony-forming unit/spleen) was 135 (range, 90--230; n = 15) and was similar to that for the cell type that provides radioprotection (180 +/- 70), indicating that these functional properties were copurified. Indirect evidence suggests that the spleen-seeding efficiency (f factor) of these cells is 0.10 and, therefore, the average purity of the sorted PHSC was 65% (range in 15 experiments, 35--110%). The sorted cells were all in the G1 or G0 phase of the cell cycle. They appeared to be undifferentiated blasts by morphological criteria. Electron microscopy revealed that the sorted cells consisted primarily of two cell types, possibly representing G0 and G1 cells. The FACS was used to deposit single selected cells into individual microwells of Terasaki trays. 32% of the sorted cells could be induced to form myeloid progeny in vitro. This procedure should be useful for direct studies on the regulation of hemopoietic cell differentiation.

show abstract

“…Estimates of daily stem cell flow into a humerus, derived from the data of several authors, are entered in Table 3, the following values being assumed: total nucleated cells in bone marrow = 20 x lo7, and in one humerus = 1 x lo7; average CFU/blood volume = 30 (Micklem, 1966); seeding efficiency of bone marrow and blood stem cells in the spleen (f) = 0.17 (Siminovitch, McCulloch & Till, 1963;Kondratenko & Chertkov, 1972). Lower values forf, proposed by several authors (Matioli, Vogel & Niewisch, 1968;Kretchmar & Conover, 1969;Lahiri, Keizer & van Putten, 1970), result in proportionately higher estimates of stem cell flow. It is also assumed for the purpose of the calculations that the bone marrow is the sole source and destination of circulating stem cells and hence that output of stem cells from the bone marrow is exactly balanced by input from the blood; these last assumptions ignore other tissues, especially the spleen, and are certainly not wholly correct (cf.…”

Section: T6t6 Marrow-injected Mice For Various Values Of P and Qr Rmentioning

confidence: 91%

Compartments and Cell Flows Within the Mouse Haemopoietic System

et al. 1975

View full text Add to dashboard Cite

Part-body irradiated CBA mice were injected with CBA-T6 bone marrow. In this way a predominantly donor population was established in the femora while the marrow of the humeri remained largely (average 94 %) of host origin. In animals examined cytologically up to 2 years later, no tendency was observed for the proportion of donor cells in the humeri to increase. Splenectomy had no effect on this. When femoral bone marrow from the experimental mice was injected into lethally (whole-body) irradiated recipients, cells originating from the primary host repopulated the lymph nodes to a disproportionate extent. Equilibration between the cell populations of femora and humeri occurred after re-exposure to 600 rad whole-body irradiation, but not after 100 rad or 350 rad; thus, regenera-

show abstract

The dilution factor of intravenously injected hemopoietic stem cells

Cited by 28 publications

References 8 publications

The Relationship Between Stem Cell Seeding Efficiency and Position in Cell Cycle

The Relationship Between Stem Cell Seeding Efficiency and Position in Cell Cycle

Isolation of murine pluripotent hemopoietic stem cells.

Compartments and Cell Flows Within the Mouse Haemopoietic System

Contact Info

Product

Resources

About