The disappearance of an hsc70 species in mung bean seed during germination: purification and characterization of the protein

Wang, Chung; Lin, Bai-Ling

doi:10.1007/bf00019947

Cited by 17 publications

(19 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hsp70 transcripts subsequently and rapidly disappeared during germination. Similar expression patterns were observed for cytosolic hsp70s of pea and a mungbean hsp70 (Wang and Lin, 1993;DeRocher and Vierling, 1995). This pattern of Hsp70 expression also closely follows the expression patterns of small heat shock proteins (Wehmeyer et al, 1996) and LEA genes (Raynal et al, 1999) in Arabidopsis, suggesting that Hsp70 expression may be regulated by a common mechanism that regulates these classes of genes during development (Galau and Hughes, 1987;Almoguera et al, 1998).…”

Section: Roles Of Hsp70s In Seed Maturationsupporting

confidence: 63%

“…In plants, comprehensive expression analysis of hsp70s has been limited (Duck et al, 1989;Denecke et al, 1991;Wang and Lin, 1993;DeRocher and Vierling, 1995;Dudley et al, 1997;Li et al, 1999) because the entire complement of hsp70 genes has not been available or known for any plant. The expression of 10 spinach hsp70 genes was studied in different temperature regimes (Li et al, 1999).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Comprehensive Expression Profile Analysis of the Arabidopsis Hsp70 Gene Family

2001

View full text Add to dashboard Cite

We isolated cDNA clones for two nuclear-encoded, organellar members of the Arabidopsis hsp70 gene family, mtHsc70-2 (AF217458) and cpHsc70-2 (AF217459). Together with the completion of the genome sequence, the hsp70 family in Arabidopsis consists of 14 members unequally distributed among the five chromosomes. To establish detailed expression data of this gene family, a comprehensive reverse transcriptase-polymerase chain reaction analysis for 11 hsp70s was conducted including analysis of organ-specific and developmental expression and expression in response to temperature extremes. All hsp70s showed 2-to 20-fold induction by heat shock treatment except cpHsc70-1 and mtHsc70-1, which were unchanged or repressed. The expression profiles in response to low temperature treatment were more diverse than those evoked by heat shock treatment. Both mitochondrial and all cytosolic members of the family except Hsp70b were strongly induced by low temperature, whereas endoplasmic reticulum and chloroplast members were not induced or were slightly repressed. Developmentally regulated expression of the heat-inducible Hsp70 in mature dry seed and roots in the absence of temperature stress suggests prominent roles in seed maturation and root growth for this member of the hsp70 family. This reverse transcriptase-polymerase chain reaction analysis establishes the complex differential expression pattern for the hsp70s in Arabidopsis that portends specialized functions even among members localized to the same subcellular compartment.

show abstract

Section: Roles Of Hsp70s In Seed Maturationsupporting

confidence: 63%

mentioning

confidence: 99%

Comprehensive Expression Profile Analysis of the Arabidopsis Hsp70 Gene Family

2001

View full text Add to dashboard Cite

show abstract

“…3A). The data indicate that hsc70s in Arabidopsis may well be developmentally regulated just as those in tomato [4] and mung bean [ 18]. The heat inducibility of HSP70-1 has been shown at the message level in the leaves [20].…”

Section: C-terminal Lo-kd Fragmentmentioning

confidence: 82%

Isolation of a cDNA encoding a 70 kDa heat-shock cognate protein expressed in vegetative tissues ofArabidopsis thaliana

Wang

Chen

et al. 1994

Plant Mol Biol

Self Cite

View full text Add to dashboard Cite

A cDNA encoding a 70 kDa heat shock 'cognate' protein (hsc70) was isolated from Arabidopsis thaliana by using a rat hsc70 cDNA as probe. Sequence analysis demonstrated the conservation of functional domains and important amino acid residues among hsc70s in plants and animals. The expression of this gene was stress-inducible, and was found at a substantial level during normal growth in root, stem, leaf and flower tissues, but not in siliques. Multiple copies of this gene exist in the Arabidopsis genome.

show abstract

“…An HSP70 has been identified in mung bean whose regulation is similar to PsHSP71.2 [46]. Like the 72 kDa pea protein it is very abundant in the cytoplasm of mature cotyledons, and disappears from both the cotyledon and hypocotyl of the seedling as germination progresses.…”

Section: Discussionmentioning

confidence: 92%

Cytoplasmic HSP70 homologues of pea: differential expression in vegetative and embryonic organs

DeRocher

Vierling

1995

Plant Mol Biol

View full text Add to dashboard Cite

Eukaryotes express several cytoplasmic HSP70 genes, and their encoded proteins participate in diverse cellular processes. Three cDNAs encoding highly expressed cytoplasmic HSP70 homologues from Pisum sativum were cloned and characterized. They were designated PsHSP71.2, PsHSC71.0, and PsHSP70b. These HSP70 genes have different expression profiles in leaves: PsHSP71.2 is observed only in response to heat stress, PsHSC71.0 is present constitutively, and PsHSP70b is weakly constitutively expressed, but induced strongly in response to heat stress. In addition to being heat induced, the PsHSP71.2 mRNA is also expressed in zygotic, but not maternal organs of developing pea seeds, while PsHSC71.0 and PsHSP70b mRNAs are present in maternal and zygotic organs throughout seed development. Immunoblot analysis of parallel protein samples detects a 70 kDa polypeptide in all samples, and a 72 kDa polypeptide that corresponds to the PsHSP71.2 gene product is observed in cotyledons beginning at mid-maturation and in axes beginning between late maturation and desiccation. This polypeptide is not detected in the seed coat. The 72 kDa polypeptide remains abundant in both cotyledons and axes through germination, but declines substantially between 48 and 72 h after the onset of inbibition. Differential control of HSP70 expression during heat stress, seed maturation, and germination is consistent with the hypothesis that there are functional distinctions between cytoplasmic HSP70s.

show abstract

The disappearance of an hsc70 species in mung bean seed during germination: purification and characterization of the protein

Cited by 17 publications

References 56 publications

Comprehensive Expression Profile Analysis of the Arabidopsis Hsp70 Gene Family

Comprehensive Expression Profile Analysis of the Arabidopsis Hsp70 Gene Family

Isolation of a cDNA encoding a 70 kDa heat-shock cognate protein expressed in vegetative tissues ofArabidopsis thaliana

Cytoplasmic HSP70 homologues of pea: differential expression in vegetative and embryonic organs

Contact Info

Product

Resources

About