The Disappearance of SpA‐IgG Antibody Complex on ‘Armed’ Lymphoid and Macrophages Cells

Abstract

The disappearance rate of rabbit IgG antibody complexed with protein A from Staphylococcus aureus (SpA) on ‘armed’ mouse and rabbit spleen lymphocytes, and on ‘armed’ mouse peritoneal macrophages, was investigated by rosette and antibody‐dependent cellular cytotoxicity (ADCC) assays. The half time of disappearance of complex from the surface of lymphoid cells was found to be 36 min in both assays. Macrophages, ‘armed’ with IgG antibody complexed with SpA, bind the corresponding antigens and subsequently lyse the target cells by extracellular and/or intracellular mechanisms. ‘Armed’ macrophages have both ADCC and phagocytosis ability, although a short half‐time of disappearance of complex was observed (8 min).