The double-stranded RNA genome of maize rough dwarfFijivirus contains both mono and dicistronic segments

Marzachı̀, Cristina; Antoniazzi, Sara; d'Aquilio, M.; Boccardo, G.

doi:10.1007/bf01877029

Cited by 21 publications

(16 citation statements)

References 22 publications

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“…The same samples run on 1.4 % agarose gels in HEPES buffer in denaturing conditions showed that the lower band resolved into two components of slightly different mobility (not shown). These results confirmed those of Marzachi et al (1992) for OuMV.…”

Section: Resultssupporting

confidence: 88%

“…OuMV has unique morphological characteristics: the bacilliform virions are composed of a series of particles with conical ends (apparently hemi-icosahedra) and cylindrical bodies 18 nm in diameter, composed of two, three, four or six double discs (Lisa et al, 1988;Matthews, 1991;Milne, 2005;Accotto & Milne, 2008). The particles consist of three linear single-stranded positive-sense RNAs and one protein with an estimated molecular weight of about 25 kDa (Lisa et al, 1988;Marzachi et al, 1992;Accotto et al, 1997). Cassava virus C (CsVC) and Epirus cherry virus (EpCV), the other Ourmiavirus species isolated from equatorial Africa and Greece, respectively (Fauquet et al, 2005), have similar particles (Aiton et al, 1988;Avgelis et al, 1989) and EpCV is known to have three RNAs and a single coat protein (CP), each slightly smaller than those of OuMV (Accotto et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of the plant virus genus Ourmiavirus and evidence of inter-kingdom reassortment of viral genome segments as its possible route of origin

Rastgou

Habibi

Izadpanah

et al. 2009

Journal of General Virology

118

122

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Ourmia melon virus (OuMV), Epirus cherry virus (EpCV) and Cassava virus C (CsVC) are three species placed in the genus Ourmiavirus. We cloned and sequenced their RNA genomes. The sizes of the three genomic RNAs of OuMV, the type member of the genus, were 2814, 1064 and 974 nt and each had one open reading frame. RNA1 potentially encoded a 97.5 kDa protein carrying the GDD motif typical of RNA-dependent RNA polymerases (RdRps). The putative RdRps of ourmiaviruses are distantly related to known viral RdRps, with the closest similarity and phylogenetic affinity observed with fungal viruses of the genus Narnaviridae. RNA2 encoded a 31.6 kDa protein which, expressed in bacteria as a His-tag fusion protein and in plants through agroinfiltration, reacted specifically with antibodies made against tubular structures found in the cytoplasm. The ORF2 product is significantly similar to movement proteins of the genus Tombusviridae, and phylogenetic analysis supported this evolutionary relationship. The product of OuMV ORF3 is a 23.8 kDa protein. This protein was also expressed in bacteria and plants, and reacted specifically with antisera against the OuMV coat protein. The sequence of the ORF3 protein showed limited but significant similarity to capsid proteins of several plant and animal viruses, although phylogenetic analysis failed to reveal its most likely origin. Taken together, these results indicate that ourmiaviruses comprise a unique group of plant viruses that might have evolved by reassortment of genomic segments of RNA viruses infecting hosts belonging to different eukaryotic kingdoms, in particular, fungi and plants.

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Section: Resultssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the plant virus genus Ourmiavirus and evidence of inter-kingdom reassortment of viral genome segments as its possible route of origin

Rastgou

Habibi

Izadpanah

et al. 2009

Journal of General Virology

118

122

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“…et al, 1991) ; a similar dicistronic structure has been found in RBSDV S7 (Azuhata et al, 1993) and MRDV S8 (Marzachı! et al, 1996).…”

Section: Introductionmentioning

confidence: 83%

“…At present, little is known of the proteins encoded by the 10 fijivirus genomic double-stranded RNA (dsRNA) segments (S1-S10), including those of RBSDV, and the nucleotide sequences of only some of them have been determined (Uyeda et al, 1990 ;Azuhata et al, 1993 ;Marzachı! et al, 1991Marzachı! et al, , 1996.…”

Section: Introductionmentioning

confidence: 99%

Detection and assignment of proteins encoded by rice black streaked dwarf fijivirus S7, S8, S9 and S10.

Isogai

Uyeda

Lee

1998

Journal of General Virology

117

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The proteins encoded by rice black streaked dwarf fijivirus (RBSDV) genomic segments 7-10 (S7-S10) were characterized. Open reading frames (ORFs) from these segments were expressed as fusion proteins in Escherichia coli. Antibodies raised against the expressed products were used as probes to determine whether the viral ORFs encode structural proteins. In Western blots, antibodies to the expressed S8 and S10 products reacted with a core capsid (65 kDa) and a major outer capsid (56 kDa) protein, respectively, while none of the antibodies to S7 and S9 products reacted with structural

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“…It exhibited the common properties of the genome segment counterparts of Fijiviruses [10][11][12][13][14][15][16][17][18][19][20][21][22][23] . Complete SRBSDV S9 had a total length of 1900 bp and a (G+C) content of 34.53%, which fell within the values obtained in other Fijiviruses from group 2 (30%-37%).…”

Section: Analysis Of Srbsdv S9 Complete Genomic Sequencementioning

confidence: 99%

Southern rice black-streaked dwarf virus: A new proposed Fijivirus species in the family Reoviridae

et al. 2008

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For the past several years, a novel dwarf disease has been observed on rice (Oryza sativa) in some regions of Guangdong Province and Hainan Province, southern China. Infected plants showed stunting, dark leaf and small enations on stem and leaf back. Typical Fijivirus viroplasma containing crystalline arrayed spherical virons approximately 70-75 nm in diameter and tubular structures were detected in ultrathin sections by an electron microscope in parenchyma phloem cells of the infected plants. The virus was transmitted to rice seedlings by white-backed planthoppers, Sogatella furcifera (Hemiptera: Delphacidae), collected in the diseased fields. Analysis of dsRNA extracts from infected plants revealed ten linear segments, which were similar to the electrophoretic profile of Rice black-streaked dwarf virus (RBSDV). RT-PCR with a single primer which matched to a linker sequence ligated to both 3′ ends of the viral genomic dsRNAs resulted in amplification of genome segments 9 (S9) and 10 (S10) cDNA products. The complete nucleotide sequences of S9 and S10 were obtained from clones of the RT-PCR amplicon exhibited characteristic properties of Fijivirus including low GC content (34.5% and 35.6%), genus conserved 5′ and 3′ termini sequences and similar genome organization. Blast searches indicated that the sequences of S9 and S10 shared 68.8%-74.9% and 67.1%-77.4% nucleotide identities with those of viruses in the Fijivirus group 2, respectively. These values were similar to those among other viruses in the Fijivirus group 2 and considerably lower than those among RBSDV isolates. Phylogenetic trees based on S9 and S10 nucleotide sequences and their putative amino acid sequences showed that this virus represented a separate branch among other Fijiviruses. The virus was also detected by a nested RT-PCR assay in corn (Zea mays), barnyard grass (Echinochloa crusgalli), Juncellus serotinus and flaccidgrass (Pennisetum flaccidum) in and/or adjacent to the infected rice fields. It is proposed that this virus be considered as a new species, Southern rice black-streaked dwarf virus, in the group 2 of the genus Fijivirus in the family Reoviridae.southern rice black-streaked dwarf virus, rice, Fijivirus, Reoviridae, genome sequence Rice black-streaked virus (RBSDV) is a species of the genus Fijivirus in the family Reoviridae [1] . It has icosahedric particles approximately 70-75 nm in diameter that contain 10 genome segments of double-stranded RNAs (dsRNA), designated as S1-S10 in increasing order of electrophoretic mobility in polyacrylamide gels. Small brown planthopper, Laodelphax striatellus, is its main natural vector, and Unkanodes sapporonus and Chilodephax albifacia can also transmit the virus with a relative low efficiency [2][3][4] . In the genus Fijivirus, 8 species, Fiji disease virus (FDV), Oat sterile dwarf virus (OSDV), Garlic dwarf virus (GDV), Nilaparvata lugens reovirus (NLRV), Mal de Río Cuarto virus (MRCV), Pangola stunt virus (PaSV), Maize rough dwarf virus (MRDV) and RBSDV were recognized [1] . Among them,

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The double-stranded RNA genome of maize rough dwarfFijivirus contains both mono and dicistronic segments

Cited by 21 publications

References 22 publications

Molecular characterization of the plant virus genus Ourmiavirus and evidence of inter-kingdom reassortment of viral genome segments as its possible route of origin

Molecular characterization of the plant virus genus Ourmiavirus and evidence of inter-kingdom reassortment of viral genome segments as its possible route of origin

Detection and assignment of proteins encoded by rice black streaked dwarf fijivirus S7, S8, S9 and S10.

Southern rice black-streaked dwarf virus: A new proposed Fijivirus species in the family Reoviridae

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