The Drug Delivery System of Centella asiatica extract-loaded Gelatin Nanoparticles using of One-step desolvation Method

Yongsirasawad, Kittithat; Yasurin, Patchanee; Asavasanti, Suvaluk; Amornraksa, Suksun; Sriariyanun, Malinee

doi:10.1145/3180382.3180403

Cited by 8 publications

(6 citation statements)

References 29 publications

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“…Moreover, the functional groups of the extract might change because of intra or inter crosslinking process during manufacturing of the gelatin nanoparticles. The crosslinker used such as glutaraldehyde could cause this intra or inter chemical reaction with gelatin molecule (Khan, 2014;Yongsirasawad et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

EFFECTS OF GELATIN AND GLUTARALDEHYDE CONCENTRATIONS ON CHARACTERISTICS OF CANTIGI (Vaccinium Varingiaefolium Miq.) EXTRACT LOADED GELATIN NANOPARTICLES AS ANTIOXIDANT

et al. 2021

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Cantigi is an endemic plant of sub-alpine area of Mount Tangkuban Parahu in Bandung, Indonesia. Previous study showed ethanol extract of young red leaves had antioxidant activity, however no information on this activity if changed into nanoparticles. The purpose of this study was to determine the effects of gelatin and glutaraldehyde concentrations on the characteristics of Cantigi extract loaded gelatin nanoparticles and to evaluate the antioxidant activity of nanoparticles. Cantigi leaves were extracted by maceration using n-hexane, ethyl acetate, and ethanol 96%. The ethanol extract was dried, made into nanoparticles by varying gelatin (0.1; 0.2; and 0.3 g) and glutaraldehyde (0.1; 0.2; and 0.3 mL) amounts, and conducted at 500 rpm and 40 °C for 3 hours. Nanoparticles were evaluated for particle size, zeta potential, morphology, and antioxidant activity. Nanoparticles with glutaraldehyde amount variation had particle sizes (PS) of 105.9±26.2; 37.1±8.7; and 32.5±7.4 nm; polydispersity indeces (PI) of 0.508; 0.717; and 0.563; zeta potential values (ZPV) of 0.55; 0.89; and 0.78 mV; and antioxidant activities (IC50) of 56.15±0.16; 53.67±0.10; and 51.57±0.39 ppm, respectively. Then, nanoparticles with gelatin amounts variation had PS of 22.5±5.1; 37.1±8.7; and 83.3±21 nm; PI of 0.604; 0.717; 0.326; ZPV of 1.27; 0.89; 0.18 mV; and antioxidant activities of 51.58±0.19; 53.67±0.12; and 55.46±0.04 ppm, respectively. Nanoparticle morphology was spherical. Cantigi leaf extract can be made into gelatin nanoparticles; the smaller the concentration of the polymer used and higher the concentration of the glutaraldehyde, the smaller the resulted particle size and increased antioxidant activity. Antioxidant activities of nanoparticles was lower than those of the extract (IC50 16.84±0.30 ppm).

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Section: Resultsmentioning

confidence: 99%

EFFECTS OF GELATIN AND GLUTARALDEHYDE CONCENTRATIONS ON CHARACTERISTICS OF CANTIGI (Vaccinium Varingiaefolium Miq.) EXTRACT LOADED GELATIN NANOPARTICLES AS ANTIOXIDANT

et al. 2021

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“…Our research represents one of the few studies in which an attempt was made to formulate an extract rather than a single compound. In the literature, there are few examples of nanoformulations of extracts using nanoparticles, such as gelatin NP of Centella asiatica and Cardamono extracts [42,43] and chitosan NP of Nigella sativa and cherry extracts [15,16]. The nanoparticles are not easy to prepare for extract delivery, due to the presence of various compounds with different polarity, but the application of nanotechnology to extract is of great interest in the phytotherapy field given the remarkable benefits that traditional medicine attributes to the synergistic action of the bioactive compounds present in phytocomplexes.…”

Section: Np and Np-poe Preparationmentioning

confidence: 99%

Comparison of Chitosan Nanoparticles and Soluplus Micelles to Optimize the Bioactivity of Posidonia oceanica Extract on Human Neuroblastoma Cell Migration

et al. 2019

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Posidonia oceanica (L.) Delile is a marine plant endemic of Mediterranean Sea endowed with interesting bioactivities. The hydroalcholic extract of P. oceanica leaves (POE), rich in polyphenols and carbohydrates, has been shown to inhibit human cancer cell migration. Neuroblastoma is a common childhood extracranial solid tumor with high rate of invasiveness. Novel therapeutics loaded into nanocarriers may be used to target the migratory and metastatic ability of neuroblastoma. Our goal was to improve both the aqueous solubility of POE and its inhibitory effect on cancer cell migration. Methods: Chitosan nanoparticles (NP) and Soluplus polymeric micelles (PM) loaded with POE have been developed. Nanoformulations were chemically and physically defined and characterized. In vitro release studies were also performed. Finally, the inhibitory effect of both nanoformulations was tested on SH-SY5Y cell migration by wound healing assay and compared to that of unformulated POE. Results: Both nanoformulations showed excellent physical and chemical stability during storage, and enhanced the solubility of POE. PM-POE improved the inhibitory effect of POE on cell migration probably due to the high encapsulation efficiency and the prolonged release of the extract. Conclusions: For the first time, a phytocomplex of marine origin, i.e., P. oceanica extract, has enhanced in terms of acqueous solubility and bioactivity once encapsulated inside nanomicelles.

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“…Kim et al prepared Centella asiatica-loaded nanocrystal suspensions and reported the drug release for 4 h . Similarly, Yongsirasawad and co-workers developed Centella asiatica extract-loaded gelatin nanoparticles using one-step desolvation method and demonstrated drug release for almost 3.5 h . Still, much work needs to be done.…”

Section: Introductionmentioning

confidence: 99%

Formulation and In Vitro Assessment of Polymeric pH-Responsive Nanogels of Chitosan for Sustained Delivery of Madecassoside

Suhail,

Chiu,

Ullah

et al. 2024

ACS Omega

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Madecassoside, a triterpenoid saponin compound mainly isolated from the gotu kola herb (Centella asiatica), shows an extensive range of biological activities, including antiapoptotic, antioxidant, anti-inflammatory, moisturizing, neuroprotective, and wound healing effects. It has been highly used in the management of eczema, skin wounds, and other diseases. Due to poor oral bioavailability, membrane permeability, and intestinal absorption, the clinical application of the madecassoside is limited. Hence, a drug carrier system is needed that not only sustains the release of the madecassoside but also overcomes the drawbacks associated with its administration. Therefore, the authors prepared novel pH-responsive chitosan-based nanogels for the sustained release of madecassoside. Free radical polymerization technique was used for cross-linking of polymer chitosan and monomer methacrylic acid in the presence of cross-linker N′,N′-methylene bis(acrylamide). The decrease in polymer crystallinity after polymerization and development of nanogels was demonstrated by XRD and FTIR analysis. The effects of nanogel contents on polymer volume, sol–gel analysis, swelling, drug loading, and release were investigated. Results indicated that high swelling and maximum release of the drug occurred at pH 7.4 compared to pH 1.2 and 4.6, indicating the excellent pH-sensitive nature of the engineered nanogels. High swelling and drug release were perceived with the integration of a high quantity of chitosan, while a decline was observed with the high integration of N′,N′-methylene bis(acrylamide) and methacrylic acid contents. The same effects of nanogel contents were shown for drug loading too. Sol fraction was reduced, while gel fraction was enhanced by increasing the chitosan load, N′,N′-methylene bis(acrylamide), and methacrylic acid. The Korsmeyer–Peppas model of kinetics was trailed by all nanogel formulations with non-Fickian diffusion. The results demonstrated that prepared nanogels can be employed for sustained release of the madecassoside.

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The Drug Delivery System of Centella asiatica extract-loaded Gelatin Nanoparticles using of One-step desolvation Method

Cited by 8 publications

References 29 publications

EFFECTS OF GELATIN AND GLUTARALDEHYDE CONCENTRATIONS ON CHARACTERISTICS OF CANTIGI (Vaccinium Varingiaefolium Miq.) EXTRACT LOADED GELATIN NANOPARTICLES AS ANTIOXIDANT

EFFECTS OF GELATIN AND GLUTARALDEHYDE CONCENTRATIONS ON CHARACTERISTICS OF CANTIGI (Vaccinium Varingiaefolium Miq.) EXTRACT LOADED GELATIN NANOPARTICLES AS ANTIOXIDANT

Comparison of Chitosan Nanoparticles and Soluplus Micelles to Optimize the Bioactivity of Posidonia oceanica Extract on Human Neuroblastoma Cell Migration

Formulation and In Vitro Assessment of Polymeric pH-Responsive Nanogels of Chitosan for Sustained Delivery of Madecassoside

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