The E4F Protein Is Required for Mitotic Progression during Embryonic Cell Cycles

Cam, Laurent Le; Lacroix, Matthieu; Ciemerych, Maria A.; Sardet, Claude; Siciński, Piotr

doi:10.1128/mcb.24.14.6467-6475.2004

Cited by 48 publications

(29 citation statements)

References 34 publications

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“…Mediator complexes were also found to play an important role in maintaining ESC identity 45 , and MED24 depletion in hESCs similarly causes cell differentiation 5 . E4f1, a transcription factor that was also previously identified in a hESC RNAi screen 5 , has been shown to be essential to mouse ESC maintenance 46 . Hence, the inappropriate splicing of OCT4 , MED24 and E4F1 transcripts and isoform switch of PRDM14 may contribute to the differentiation phenotype induced by SON deficiency in hESCs.…”

Section: Discussionmentioning

confidence: 92%

SON connects the splicing-regulatory network with pluripotency in human embryonic stem cells

et al. 2013

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Human embryonic stem cells (hESCs) harbour the ability to undergo lineage-specific differentiation into clinically relevant cell types. Transcription factors and epigenetic modifiers are known to play important roles in the maintenance of pluripotency of hESCs. However, little is known about regulation of pluripotency through splicing. In this study, we identify the spliceosome-associated factor SON as a factor essential for the maintenance of hESCs. Depletion of SON in hESCs results in the loss of pluripotency and cell death. Using genome-wide RNA profiling, we identified transcripts that are regulated by SON. Importantly, we confirmed that SON regulates the proper splicing of transcripts encoding for pluripotency regulators such as OCT4, PRDM14, E4F1 and MED24. Furthermore, we show that SON is bound to these transcripts in vivo. In summary, we connect a splicing-regulatory network for accurate transcript production to the maintenance of pluripotency and self-renewal of hESCs.

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Section: Discussionmentioning

confidence: 92%

SON connects the splicing-regulatory network with pluripotency in human embryonic stem cells

et al. 2013

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“…These findings reinforce the importance of these motifs in LRG expression (Park et al, 2006). The GSEA analysis also identified TF‐binding motifs not previously LTP‐related: the developmentally regulated paired box gene 3 (PAX3), E4F transcription 1 (E4F1) implicated in cell‐cycle progression (Le Cam et al, 2004), and POU domain TF (POU2F1) implicated in transcription of small nuclear RNA as well as Alzheimer's disease (Taguchi et al, 2005). These are new candidate TF‐binding motifs that may contribute to the coordinate regulation of LRG transcription.…”

Section: Resultsmentioning

confidence: 99%

Homeostatic, circadian, and emotional regulation of sleep

Saper¹,

Cano²,

Scammell³

2005

J of Comparative Neurology

336

237

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A good night's sleep is one of life's most satisfying experiences, while sleeplessness is stressful and causes cognitive impairment. Yet the mechanisms that regulate the ability to sleep have only recently been subjected to detailed investigation. New studies show that the control of wake and sleep emerges from the interaction of cell groups that cause arousal with other nuclei that induce sleep such as the ventrolateral preoptic nucleus (VLPO). The VLPO inhibits the ascending arousal regions and is in turn inhibited by them, thus forming a mutually inhibitory system resembling what electrical engineers call a "flip-flop switch." This switch may help produce sharp transitions between discrete behavioral states, but it is not necessarily stable. The orexin neurons in the lateral hypothalamus may help stabilize this system by exciting arousal regions during wakefulness, preventing unwanted transitions between wakefulness and sleep. The importance of this stabilizing role is apparent in narcolepsy, in which an absence of the orexin neurons causes numerous, unintended transitions in and out of sleep and allows fragments of REM sleep to intrude into wakefulness. These influences on the sleep/wake system by homeostatic and circadian drives, as well as emotional inputs, are reviewed. Understanding the pathways that underlie the regulation of sleep and wakefulness may provide important insights into how the cognitive and emotional systems interact with basic homeostatic and circadian drives for sleep.

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“…In particular, the percentage of HK genes containing E4F motifs at the maximum peak position was 6%, twice that of RT genes. E4F is a ubiquitously expressed protein reported to be important for mitotic progression [ 24 ]. The other motif that was not significant in RT genes was the E2F binding site, which was also about twice as frequent in HK genes than in RT genes.…”

Section: Resultsmentioning

confidence: 99%

Positional bias of general and tissue-specific regulatory motifs in mouse gene promoters

2007

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Background: The arrangement of regulatory motifs in gene promoters, or promoter architecture, is the result of mutation and selection processes that have operated over many millions of years. In mammals, tissue-specific transcriptional regulation is related to the presence of specific protein-interacting DNA motifs in gene promoters. However, little is known about the relative location and spacing of these motifs. To fill this gap, we have performed a systematic search for motifs that show significant bias at specific promoter locations in a large collection of housekeeping and tissue-specific genes.

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The E4F Protein Is Required for Mitotic Progression during Embryonic Cell Cycles

Cited by 48 publications

References 34 publications

SON connects the splicing-regulatory network with pluripotency in human embryonic stem cells

SON connects the splicing-regulatory network with pluripotency in human embryonic stem cells

Homeostatic, circadian, and emotional regulation of sleep

Positional bias of general and tissue-specific regulatory motifs in mouse gene promoters

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