The early evaluation of induced osteoarthritis in rats with 99Tcm-pertechnetate scans

Mh, Huang; Hj, Ding; Cc, Yang; Chai, Chee‐Yin; Rc, Yang

doi:10.1097/00006231-199606000-00013

Cited by 9 publications

(15 citation statements)

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“…OA was induced in the right knees of rats in the OA and OA plus PTH groups with intraarticular injections of 20 l of 4% papain solution and 20 l of 0.03M cysteine. The injections were given with a 26-gauge needle via the patellar tendon on days 1, 4, and 7 of the experiment (13). In the OA plus PTH group, after OA induction, the right knees were injected intraarticularly with 40 l of 10 nM PTH(1-34) every 3 days until rats were killed.…”

Section: Methodsmentioning

confidence: 99%

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Parathyroid hormone 1–34 inhibits terminal differentiation of human articular chondrocytes and osteoarthritis progression in rats

Chang¹,

Chang²,

Hung³

et al. 2009

Arthritis & Rheumatism

100

120

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Objective. Parathyroid hormone 1-34 (PTH[1-34]), a parathyroid hormone analog, shares the same receptor, PTH receptor 1, with parathyroid hormone-related peptide (PTHrP). This study was undertaken to address the hypothesis that PTH(1-34) inhibits terminal differentiation of articular chondrocytes and in turn suppresses the progression of osteoarthritis (OA).Methods. We studied the effect of PTH(1-34) on human articular chondrocytes with azacytidine (azaC)-induced terminal differentiation in vitro and on papaininduced OA in the knee joints of rats. In the in vitro study, we measured the levels of messenger RNA for SOX9, aggrecan, type II collagen, type X collagen, alkaline phosphatase (AP), Indian hedgehog (IHH), Bcl-2, and Bax by real-time polymerase chain reaction, levels of glycosaminoglycan (GAG) by dimethylmethylene blue assay, and rate of apoptosis by TUNEL staining. In the in vivo study, we evaluated the histologic changes in GAG, type II collagen, type X collagen, and chondrocyte apoptosis in the articular cartilage of rat knees.Results. AzaC induced terminal differentiation of human chondrocytes, including down-regulation of aggrecan, type II collagen, and GAG and up-regulation of type X collagen, alkaline phosphatase, and IHH. Apoptosis was reversed by 3-10 days of treatment with 10 nM PTH(1-34). SOX9 expression was not changed by either azaC or PTH(1-34) treatment. Bcl-2 and Bax were up-regulated on day 10 and day 14, respectively, after azaC induction of terminal differentiation, but PTH(1-34) treatment did not reverse this effect. Furthermore, PTH(1-34) treatment reversed papaininduced OA changes (decreasing GAG and type II collagen, and increasing type X collagen and chondrocyte apoptosis) in the knee joints of rats.Conclusion. Our findings indicate that PTH(1-34) inhibits the terminal differentiation of human articular chondrocytes in vitro and inhibits progression of OA in rats in vivo, and may be used to treat OA.

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Section: Methodsmentioning

confidence: 99%

“…Therefore, azaC induction of terminal differentiation was performed in cultured cells at the proliferating stage. The in vivo effect of PTH on articular cartilage during the progression of OA was tested using a papain-induced model of OA in rats (13)(14)(15).…”

mentioning

confidence: 99%

Parathyroid hormone 1–34 inhibits terminal differentiation of human articular chondrocytes and osteoarthritis progression in rats

Chang¹,

Chang²,

Hung³

et al. 2009

Arthritis & Rheumatism

100

120

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“…The technetium 99m ( 99m Tc)-uptake measurement was made as previously described 21,22 to assess the severity of induced arthritis in the rat. This was performed in each animal both before (2 wks after induction) and after laser treatment, and was repeated 2 mos later at follow-up.…”

Section: Technetium-99m-uptake Measurementmentioning

confidence: 99%

“…For scintigraphic assessment, 1 mCi/kg radioactivity of 99m TcO 4 -in 0.2 ml of saline was injected subcutaneously into the posterior neck region. 21 The 99m Tcuptake was measured by bone scan (GCA-90B gamma-camera, Toshiba, Tokyo, Japan). The peak ratio of radioactivity uptake of right and left knees was chosen as the severity index of induced osteoarthritis.…”

Section: Technetium-99m-uptake Measurementmentioning

confidence: 99%

“…The increase of the severity index has been shown in past studies to be progressive with time and was closely correlated with pathologic changes in articular cartilage. 21,22 After bone scan measurement, 42 rats with radioactivity ratio ranges from 1.3 to 1.7 were selected and randomly assigned into two groups, a laser-treated group (Gl) and control group (Gc), by a secure system of sequentially opaque sealed envelopes. Gl (21 rats) received laser treatment, and Gc (21 rats) acted as a control.…”

Section: Technetium-99m-uptake Measurementmentioning

confidence: 99%

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Effects of Helium-Neon Laser on Levels of Stress Protein and Arthritic Histopathology in Experimental Osteoarthritis

Lin

Huang

Chai

et al. 2004

American Journal of Physical Medicine & Rehabilitation

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Effects of helium–neon laser on the mucopolysaccharide induction in experimental osteoarthritic cartilage

Lin

Huang

Chai

2006

Osteoarthritis and Cartilage

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The early evaluation of induced osteoarthritis in rats with 99Tcm-pertechnetate scans

Cited by 9 publications

References 0 publications

Parathyroid hormone 1–34 inhibits terminal differentiation of human articular chondrocytes and osteoarthritis progression in rats

Parathyroid hormone 1–34 inhibits terminal differentiation of human articular chondrocytes and osteoarthritis progression in rats

Effects of Helium-Neon Laser on Levels of Stress Protein and Arthritic Histopathology in Experimental Osteoarthritis

Effects of helium–neon laser on the mucopolysaccharide induction in experimental osteoarthritic cartilage

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