The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration

Yamano, Seiichi; Haku, Ken; Yamanaka, Takuto; Dai, Jisen; Takayama, Tadahiro; Shohara, Ryutaro; Tachi, Keita; Ishioka, Mika; Hanatani, Shigeru; Karunagaran, Sanjay; Wada, Keisuke; Moursi, Amr M.

doi:10.1016/j.biomaterials.2013.12.006

Cited by 53 publications

(46 citation statements)

References 39 publications

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“…However, recombinant human PDGF-BB is rapidly released from the beta-tri calcium phosphate within 90 min [33]. Noncross-linked bovine type I collagen loaded with PDGF-BB or GDF-5 improved bone healing and release of recombinant human PDGF-BB over several days [34, 35]. The membrane was loaded by soaking for 1 h in the growth factor solution.…”

Section: Discussionmentioning

confidence: 99%

Release kinetics and mitogenic capacity of collagen barrier membranes supplemented with secretome of activated platelets - the in vitro response of fibroblasts of the periodontal ligament and the gingiva

et al. 2017

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BackgroundPlatelet preparations can stimulate the healing process and have mitogenic properties. We hypothesized that collagen barrier membranes (CBM), clinically used in guided bone regeneration and guided tissue regeneration, can serve as carriers for platelet secretome.MethodsSecretome was generated from washed platelets and unwashed platelets (washed/unwashed PSEC) and lyophilized onto CBM. Overall appearance of CBM was evaluated by scanning electron microscopy. The impact of PSEC on cell attachment was measured based on fluorescence microscopy with DiI-labeled cells. To assess the release kinetics, supernatants of CBM were collected and medium was replaced at hour 1–48. The mitogenic effect was evaluated with periodontal fibroblasts. Furthermore, the release of total protein, platelet-derived growth factor (PDGF)-BB, and transforming growth factor (TGF) β1 was measured.ResultsCBM overall appearance and cell attachment was not modulated by PSEC. Supernatants taken after one hour induced a mitogenic response in fibroblasts and showed the highest levels of total protein, TGFβ1 and PDGF-BB. These effects decreased rapidly in subsequent supernatants. While supernatants of CBM loaded with unwashed PSEC induced a stronger mitogenic response than supernatants of CBM loaded with washed PSEC this difference between the PSEC preparations was not observed when cells were seeded on 48–hours-washed CBM.ConclusionsCBM release platelet-derived factors in continuously declining release kinetics.

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Section: Discussionmentioning

confidence: 99%

Release kinetics and mitogenic capacity of collagen barrier membranes supplemented with secretome of activated platelets - the in vitro response of fibroblasts of the periodontal ligament and the gingiva

et al. 2017

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“…The traditional GTR membrane was mainly used as a barrier to prevent epithelial cell downgrowth into defects before new bone formation. Recently, a functional drug delivery membrane has been developed to control the release of drugs or growth factors, thus enhancing new bone regeneration (Lee,Lee,Wang,6 Chen & Lai, 2016;Monteiro et al, 2015;Yamano et al, 2014). Controlled and sustained release of antibiotics and/or growth factors has become a promising approach to control disease and enhance bone regeneration.…”

Section: Introductionmentioning

confidence: 98%

A functional chitosan membrane with grafted epigallocatechin-3-gallate and lovastatin enhances periodontal tissue regeneration in dogs

Lee

Lin

et al. 2016

Carbohydrate Polymers

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“…[1] However, as conventional membrane has inevitable structural, mechanical, and bio-functional limitations, an ideal membrane with a suitable degradation rate for space-maintaining and desirable bone inductivity to accelerate bone formation is yet to be discovered. [2] A wide variety of biodegradable barriers, such as those based on polymers, chitosan, and collagens [3][4][5][6][7][8] that do not need surgical removal [1,9] have been applied to protect regenerative site. To date, as the barrier membranes for required space maintenance and a shield against fibroblasts, collagen-based membranes have limited success because of fast in vivo degradation.…”

Section: Introductionmentioning

confidence: 99%

Preparation and preliminaryin vitroevaluation of a bFGF-releasing heparin-conjugated poly(ε-caprolactone) membrane for guided bone regeneration

Cao

Song

Yao

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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In an effort to improve guided bone regeneration (GBR), we successfully fabricated a novel basic fibroblast growth factor (bFGF)-releasing heparin-conjugated poly(ε-caprolactone) membrane (hep-PCL/bFGF). This material has a porous microstructure with smooth and rough pore walls before and after heparinization, respectively. Our FTIR analyses indicated that chemical bonds were formed between PCL and heparin with a new amide C=O band at 1660 cm(-1) and a band at 3400 cm(-1) that can be attributed to -OH stretching in cross-linked heparin. We showed that bFGF was released from hep-PCL/bFGF in a continuous pattern, which remained for 3 weeks. We evaluated MG63 cell proliferation and biocompatibility of GBR membrane by a CCK-8 assay and a live/dead assay. The CCK-8 results revealed that the hep-PCL/bFGF group had superiority compared to other groups. Furthermore, cell morphology of hep-PCL membrane exhibited larger projected areas than those of PCL surfaces based on scanning electron microscopy analysis and immunofluorescent staining of cell cytoskeleton and vinculin expression. Our alkaline phosphatase activity assay also confirmed better performance of the hep-PCL/bFGF group. These results suggested that this novel hep-PCL/bFGF membrane is suitable for osteoblast-like cells to attach, proliferate, and differentiate. Therefore, the hep-PCL/bFGF membrane has potential to be a biodegradable membrane for GBR and warrants further investigation.

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The effect of a bioactive collagen membrane releasing PDGF or GDF-5 on bone regeneration

Cited by 53 publications

References 39 publications

Release kinetics and mitogenic capacity of collagen barrier membranes supplemented with secretome of activated platelets - the in vitro response of fibroblasts of the periodontal ligament and the gingiva

Release kinetics and mitogenic capacity of collagen barrier membranes supplemented with secretome of activated platelets - the in vitro response of fibroblasts of the periodontal ligament and the gingiva

A functional chitosan membrane with grafted epigallocatechin-3-gallate and lovastatin enhances periodontal tissue regeneration in dogs

Preparation and preliminaryin vitroevaluation of a bFGF-releasing heparin-conjugated poly(ε-caprolactone) membrane for guided bone regeneration

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