The Effect of a Topical Agent With Mupirocin on Multidrug-Resistant Clinical Strains of S. Aureus Isolated From Patients With Common Dermatoses

Abstract

Introduction. Therapy of infectious lesions is complicated due to the growing resistance of the main pathogens of pyoderma, especially S. aureus. A key component of the treatment of diseases with mild and moderate severity is the use of local antistaphylococcal drugs that can reach maximum doses in the focus of secondary infection, minimizing the side effects of antibiotics. The aim is to evaluate the antibacterial activity of the ointment with mupirocin in relation to polyresistant clinical strains of S. aureus, isolated from patients with common dermatoses. Materials & Methods. Using the disk-diffusion method, the antibiotic resistance profile of 142 clinical strains of S. aureus was studied with the revision of the biofilming ability of 36 clinical strains of S. aureus using the method of adhesion to polystyrene. It was evaluated the therapeutic efficacy of the ointment with mupirocin in the treatment of these diseases aggravated by secondary infection. Results. According to the monitoring, the share of multi drug resistance strains (multi drug resistance, MDR) was 54.2 %, and extensive drug resistance (extreme drug resistance, XDR) – 4.9 %. Exactly 49 clinical MDR strains were selected for detailed determination of the antibacterial action of mupirocin. It was found that mupirocin actively suppressed the growth of both MRSA and MSSA. No clinical strain resistant to mupirocin was detected. It was found that 20.0% of clinical strains had a high ability to biofilm formation, medium - 60.0%, low - 20.0%. The ointment with mupirocin exhibits a highly effective antibacterial effect against clinical MRSA. As a result of the use of an ointment with mupirocin for external treatment of patients with common dermatoses during an exacerbation, regression of clinical signs of pyococcal infection was noted in 93.0 % of individuals. Conclusion. As a result of the performed researches it is proved that Mupirocin has rather expressed antistaphylococcal activity, including against MRSA of strains with high ability to formation of biofilms. The level of antimicrobial action of Mupirocin allows to recommend its use as a drug for external therapy of microbial complications of common dermatoses, which will increase the effectiveness of therapy during exacerbation, avoid side effects, reduce systemic pharmacological load, achieve sustainable, long-term remission of the patients. Keywords: clinical strains of S. aureus, antibiotic resistance, external antibiotic therapy, mupirocin.