A serious problem in patients with atopic dermatitis (AD) is the frequent attachment of a secondary skin infection. Among the microbes colonizing the skin of patients suffering from AD, S. aureus takes the lead. According to different authors, from the skin of 80–95 % of patients are sown Staphylococcus aureus. The survival of bacteria in a biotope is promoted by the persistent properties of microorganisms. Aim of the research: to determine the adhesive properties and antilysozyme activity of clinical strains of staphylococci isolated from the skin of patients with allergic dermatosis. The study included 50 patients with atopic dermatitis and 20 practically healthy individuals, from which 140 laboratory strains of staphylococci were isolated: 101 strains from patients with AD and 39 control strains. Bacteriological studies to isolate microorganisms and determine a number of pathogenic characteristics were carried out using the methods of classical bacteriology. The severity of antilysozyme activity (ALA) and adhesive properties of strains isolated from affected areas of the skin was significantly higher than in cultures isolated from intact skin areas, both qualitatively and quantitatively. The obtained data made it possible to assume a certain complicating role of these factors on the course of AD.

The involvement of complex immune mechanisms in the pathological process in psoriasis actualizes immunological research, since the clinic and course of psoriasis largely depends on the degree of immunological disorders and the imbalance of cytokine regulation of intercellular interactions. The dynamics of these processes during sanogenesis is of some interest. The purpose of the work is to study the cytokine status and its changes in patients with psoriasis depending on the type of treatment. Materials and methods. Cytokine status (IL‑6, IL‑8, IL‑10, IFN-γ and TNF-α levels in blood sera) of 60 patients with psoriasis and 17 practically healthy individuals was determined using immunoenzymatic analysis. Results and discussion. According to the results of the study, a significant imbalance of the indicators of the cytokine status of patients with psoriasis was established, which is difficult to explain by the activation or suppression of any one-cytokine pathway. The obtained data indicate the need to take into account these indicators in the treatment of patients, especially with the use of immunosuppressive agents. Conclusions. When studying the cytokine status of patients with psoriasis before treatment, a significant increase in the production of pro-inflammatory cytokines IL‑6, IL‑8, TNF-α, IFN-γ and the anti-inflammatory cytokine IL‑10 by blood cells was found. The use of therapy methods, which in particular contained immunosuppressive drugs, led to a decrease in pro-inflammatory cytokines and a certain normalization of anti-inflammatory ones. At the same time, a tendency towards a lower effect of GCS on the normalization of cytokine status was noted in comparison with cytostatic drugs in comparison with therapy without immunosuppressive agents.

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The objective: the study of genetic affinity of S. aureus strains isolated from patients with AD Materials and methods. Genetic variability of the 79 S. aureus isolates was evaluated according to the results of the RAPD-PCR study. Results. It was revealed a generally high genetic polymorphism of S. aureus. It was established that with an increase in the severity of АD, the monotypism of pathogen isolates grows (p ≤ 0,05) from three different genetic clusters in patients with AD with a mild disease course to only one cluster with a severe disease course. A high level of genetic affinity was established between S. aureus strains isolated from different biotopes from the same patients, which for strains isolated from the nasal mucosa and from locus morbi reached (79,5 ± 1,6)%, and for strains isolated from intact and affected skin are as increased from (75,1 ± 4,4)% to (98,8 ± 0,8)% in parallel with the increase in the severity of the disease course in patients with AD from mild to severe forms, respectively (p ≤ 0,05). Conclusions. Both the generally high genetic polymorphism of S. aureus strains isolated from allergodermatosеs patients and the high affinity of isolates from different biotopes of the same patient, justify the auto-origin of the strains of staphylococci colonizing locus morbi.

Introduction. Therapy of infectious lesions is complicated due to the growing resistance of the main pathogens of pyoderma, especially S. aureus. A key component of the treatment of diseases with mild and moderate severity is the use of local antistaphylococcal drugs that can reach maximum doses in the focus of secondary infection, minimizing the side effects of antibiotics. The aim is to evaluate the antibacterial activity of the ointment with mupirocin in relation to polyresistant clinical strains of S. aureus, isolated from patients with common dermatoses. Materials & Methods. Using the disk-diffusion method, the antibiotic resistance profile of 142 clinical strains of S. aureus was studied with the revision of the biofilming ability of 36 clinical strains of S. aureus using the method of adhesion to polystyrene. It was evaluated the therapeutic efficacy of the ointment with mupirocin in the treatment of these diseases aggravated by secondary infection. Results. According to the monitoring, the share of multi drug resistance strains (multi drug resistance, MDR) was 54.2 %, and extensive drug resistance (extreme drug resistance, XDR) – 4.9 %. Exactly 49 clinical MDR strains were selected for detailed determination of the antibacterial action of mupirocin. It was found that mupirocin actively suppressed the growth of both MRSA and MSSA. No clinical strain resistant to mupirocin was detected. It was found that 20.0% of clinical strains had a high ability to biofilm formation, medium - 60.0%, low - 20.0%. The ointment with mupirocin exhibits a highly effective antibacterial effect against clinical MRSA. As a result of the use of an ointment with mupirocin for external treatment of patients with common dermatoses during an exacerbation, regression of clinical signs of pyococcal infection was noted in 93.0 % of individuals. Conclusion. As a result of the performed researches it is proved that Mupirocin has rather expressed antistaphylococcal activity, including against MRSA of strains with high ability to formation of biofilms. The level of antimicrobial action of Mupirocin allows to recommend its use as a drug for external therapy of microbial complications of common dermatoses, which will increase the effectiveness of therapy during exacerbation, avoid side effects, reduce systemic pharmacological load, achieve sustainable, long-term remission of the patients. Keywords: clinical strains of S. aureus, antibiotic resistance, external antibiotic therapy, mupirocin.