No abstract

The aim is to study the state of indicators of oxidative modification of proteins in the blood plasma of patients with psoriasis vulgaris. Materials and Methods. 18 patients with psoriasis vulgaris (average age – 44.4 years) and a group of healthy donors – 22 persons, who made up the control group – were examined. Oxidative modification of proteins was determined in blood plasma by reaction with 2,4-dinitrophenylhydrazine. Results In patients with psoriasis vulgaris, significant violations of the processes of oxidative modification of blood plasma proteins (spontaneous and metal-catalyzed) were detected. Conclusions. In patients with psoriasis vulgaris, the period of exacerbation of the disease, is accompanied by carbonyl stress, which is confirmed by a significant increase in the blood content of aldehyde and ketone phenylhydrazones.

Psoriasis is considered a systemic disease with damage not only to the skin, but also to functional and morphological disorders of other organs and systems of the body, which significantly worsen as a result of transmission by patients of COVID‑19, therefore, the determination of assessment markers of disease severity in the post-covid period is of some interest. The purpose of the work is to determine the most informative prognostic markers of the severity of psoriasis in the blood sera of patients who have suffered from COVID‑19 in the post-epidemic period. Materials and methods. With the help of immunoenzymatic analysis and biochemical studies, the levels of IL‑6, CRP, troponin, ferritin, procalcitonin, hemostasiological indicators, as well as the functional state of the liver in the blood sera of 34 patients with psoriasis and 20 practically healthy individuals were determined. Results and discussion. Based on the results of the study, the most informative assessment markers were established in the sera of patients with psoriasis in the post-covid period. The obtained data indicate the need to take into account these indicators in the treatment of patients, especially when using immunosuppressive agents. Conclusions. When studying the functions of the hepatobiliary system in patients with psoriasis in the post-covid period, deviations from the norm of the hepatocellular type (increased levels of AlT, AsT) are observed, and in patients with psoriasis – of the mixed type (increased levels of AlT, AsT and alkaline phosphatase). When studying the state of the hemostasis system, changes in hemostasiological parameters were noted: an increase in the activated partial thromboplastin time [(30.38 ± 2.21) sec], an increase in PTI and a slight increase in fibrinogen content. It was established that the most indicative markers of the presence of an inflammatory process are the concentrations of CRP, IL 6 and D-dimer, the levels of which were higher than the normal level in all studied groups with the maximum increase in the group of patients with psoriasis in the post-epidemic period [(40.8 ± 1, 6) mg/l, against (4.8 ± 1.7) mg/l; (17.9 ± 3.4) pg/ml vs. (2.7± 1.1) pg/ml and (1321.3±634.3) ng/ml vs. (286±76.4 ng/ml), respectively, p ≤ 0.05; p ≤ 0.005], which may indicate a systemic inflammatory response.

The article presents literature data on the study of the content of pro- and anti-inflammatory cytokines in psoriasis and their relationship with the activity of the dermatological pathological process. Cytokines play one of the key roles in the development and maintenance of psoriatic lesions. Processes that are accompanied by pathological changes in lymphocytes, neutrophils, keratinocytes, endothelial and other cells in psoriasis are induced by the combined effects of many cytokines, chemokines, and other mediators of inflammation. It is possible that an imbalance in the system of pro- and anti-inflammatory cytokines of systemic and local origin may play a key role in this pathology.

The involvement of complex immune mechanisms in the pathological process in psoriasis actualizes immunological research, since the clinic and course of psoriasis largely depends on the degree of immunological disorders and the imbalance of cytokine regulation of intercellular interactions. The dynamics of these processes during sanogenesis is of some interest. The purpose of the work is to study the cytokine status and its changes in patients with psoriasis depending on the type of treatment. Materials and methods. Cytokine status (IL‑6, IL‑8, IL‑10, IFN-γ and TNF-α levels in blood sera) of 60 patients with psoriasis and 17 practically healthy individuals was determined using immunoenzymatic analysis. Results and discussion. According to the results of the study, a significant imbalance of the indicators of the cytokine status of patients with psoriasis was established, which is difficult to explain by the activation or suppression of any one-cytokine pathway. The obtained data indicate the need to take into account these indicators in the treatment of patients, especially with the use of immunosuppressive agents. Conclusions. When studying the cytokine status of patients with psoriasis before treatment, a significant increase in the production of pro-inflammatory cytokines IL‑6, IL‑8, TNF-α, IFN-γ and the anti-inflammatory cytokine IL‑10 by blood cells was found. The use of therapy methods, which in particular contained immunosuppressive drugs, led to a decrease in pro-inflammatory cytokines and a certain normalization of anti-inflammatory ones. At the same time, a tendency towards a lower effect of GCS on the normalization of cytokine status was noted in comparison with cytostatic drugs in comparison with therapy without immunosuppressive agents.