The effect of adding Rosmarinic and Ascorbic acids to vitrification media on fertilization rate of the mice oocyte: An experimental study

Borjizadeh, Abdollah; Ahmadi, Hamid; Daneshi, Erfan; Roshani, Daem; Fathi, Fardin; Abdi, Mahdad; Nasseri, Sherko; Abouzaripour, Morteza

doi:10.18502/ijrm.v17i3.4518

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…The maturation culture medium was prepared in our laboratory after Parrish et al (1986) protocol with minor modifications: TCM 199 HEPES modification medium, (M2520) with 10% estrus cow serum (ECS) and 0.88UI/mL FSH (F8174); group C (control), in experimental groups we added 105 µM rosmarinic acid (536954)); group R and 0.5 mM ascorbic acid; group A, concentrations chosen based on the best results from literature obtained by other molecular evaluation [ 32 , 34 , 39 ]. The number of COCs submitted to in vitro maturation according to the morphological classification was 81 COCs class 1, 98 COCs class 2 and 214 COCs class 3.…”

Section: Methodsmentioning

confidence: 99%

Utilization of Rosmarinic and Ascorbic Acids for Maturation Culture Media in Order to Increase Sow Oocyte Quality Prior to IVF

et al. 2021

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The beneficial effect of antioxidant supplementation in maturation culture media of sow oocytes was evaluated by the expression quantification of apoptotic genes and the genes that ensure stability of germ cells during fertilization. The oocytes were cultivated for 44 h in conventional medium (C) or in medium supplemented with 105 µM rosmarinic acid (R) and 0.5 mM ascorbic acid (A) and classified into three quality classes by morphological observation from which the total RNA was isolated. The gene expression of Ptx3 and the apoptotic regulator p53, Bax and BCL-2 were evaluated by quantitative PCR technique. The decreased expression of the Bax gene in the A and R groups, compared to the control, indicates a protective role of antioxidants in the cells. Cell homeostasis was maintained, as reflected in the ratio of Bax/Bcl-2 in class I COCs (cumulus-oocyte complex) regardless of the experimental group, indicating minimum cellular stress. The expression of p53 genes was higher in all class III COC, but in A1 and R1 the expression was lower than in C1, and a similar Ptx-3 gene decreased significantly in groups A1, A2, A3 and R1 compared with control groups. Antioxidant supplementation showed beneficial effects on all morphological classes of pig COCs.

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Section: Methodsmentioning

confidence: 99%

Utilization of Rosmarinic and Ascorbic Acids for Maturation Culture Media in Order to Increase Sow Oocyte Quality Prior to IVF

et al. 2021

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“…In addition, the use of antioxidants should also be investigated, during vitrification and after warming, since both cryopreservation and in vitro culture are known to cause oxidative stress [71,72]. Several compounds, often of natural origin (e.g., vitamins, plant-derived molecules, melatonin), were studied in different species, and an improvement in the developmental competence of vitrified oocyte was often achieved [73][74][75][76][77][78][79].…”

Section: During Vitrification-warmingmentioning

confidence: 99%