The effect of amino acids on intestinal smooth muscle related to their content in blood and tissue

Huizinga, Jan D.

doi:10.1016/0014-2999(80)90012-6

Cited by 6 publications

(2 citation statements)

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“…The concentrations of amino acids used in the experiments de scribed above were pharmacological, but the concentrations in MEM range from 2 mmol/1 for glutamine to 0.05 mmol/1 for tryptophan. Glutamine produced significant contractions of the guinea pig taenia coli and had small effects on the stomach fundus; however, the exact concentration used is not known [6], Glutamine was not tested on the rabbit distal colon. No reports concerning the effects of amino acids on bladder contractile function could be found outside of this communica tion.…”

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“…Intestinal smooth mus cle appears to be sensitive to amino acids, including some of those present in MEM. A study by Huizinga [6] investigated the effects of 26 amino acids on contractile responses of the guinea pig taenia coli and stomach fun dus. Below 1 mmol/1, none of the amino acids tested had any effects on muscle tension, but as the concentration was increased to 10 mmol/1 muscle contractions were pro duced.…”

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Influence of the Bathing Medium on the Contractile Responses of the Rabbit Urinary Bladder

Samadzadeh

Tong²,

Wein

et al. 1992

Pharmacology

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This study examined contractile responses of the in vitro rabbit whole-bladder preparation to field stimulation, bethanechol and KCl in Tyrode’s solution and minimum essential medium (MEM). We found frequency-dependent increases in intravesical pressure in bladders incubated in Tyrode’s solution and MEM. However, bladders incubated in MEM consistently responded with greater increases in intravesical pressure compared to those incubated in Tyrode’s solution. Similarly, there were frequency-dependent increases in the rate of pressure generation in bladders incubated in Tyrode’s solution and MEM, and bladders incubated in MEM responded with greater rates of pressure generation than those incubated in Tyrode’s solution at frequencies of 1 2 and 4 Hz. In addition, there were significant increases in intravesical pressure generated in response to administration of 500 mmol/l bethanechol and 186.4 mmol/l KCl in bladders incubated in MEM compared to Tyrode’s solution but no changes in the rate of pressure generation. The influence of L-methionine, one of the constituents of MEM, on the responses of whole bladders to nerve stimulation was also investigated. L-methionine at concentrations of 0.1 and 1.0 mmol/l had no effects on the increase in intravesical pressure or rate of pressure generation following nerve stimulation. It is speculated that the increases in contractile responsiveness of bladders incubated in MEM are related to the combination of amino acids, vitamins and other constituents of the cell culture medium.

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