The effect of apricots on the experimental cataract model formed by sodium selenite

Doğanay, Selim; Düz, Cem; Fırat, Penpe Gül; Çankaya, Cem; Kütükde, Derya; Çiğremiş, Yılmaz

doi:10.1016/j.fct.2012.12.061

Cited by 8 publications

(9 citation statements)

References 30 publications

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“…3) when compared to control group lenses, indicating increased lipid peroxidation in the Na 2 SeO 3 group. This observation is consistent with other studies that reported increased lipid peroxidation upon treatment with Na 2 SeO 3 [13, 14, 19, 23, 30, 60, 61]. NACA was able to decrease the lipid peroxidation induced by Na 2 SeO 3 by providing adequate amounts of GSH, which is a substrate for the glutathione peroxidase-catalyzed reduction of hydroperoxides.…”

Section: Discussionsupporting

confidence: 92%

“…This is likely achieved through several processes: supplying cysteine for GSH biosynthesis, reducing extracellular cystine to cysteine [59], and converting GSSG to GSH by non-enzymatic thiol disulfide exchange [31]. Our results are in agreement with previous studies that reported a decrease in GSH levels upon Na 2 SeO 3 treatment in animal models [14, 19, 60–64]. …”

Section: Discussionsupporting

confidence: 91%

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Prevention and reversal of selenite-induced cataracts by N-acetylcysteine amide in Wistar rats

Maddirala¹,

Tobwala²,

Karacal³

et al. 2017

BMC Ophthalmol

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BackgroundThe present study sought to evaluate the efficacy of N-acetylcysteine amide (NACA) eye drops in reversing the cataract formation induced by sodium selenite in male Wistar rat pups.MethodsForty male Wistar rat pups were randomly divided into a control group, an N-acetylcysteine amide-only group, a sodium selenite-induced cataract group, and a NACA-treated sodium selenite-induced cataract group. Sodium selenite was injected intraperitoneally on postpartum day 10, whereas N-acetylcysteine amide was injected intraperitoneally on postpartum days 9, 11, and 13 in the respective groups. Cataracts were evaluated at the end of week 2 (postpartum day 14) when the rat pups opened their eyes. N-acetylcysteine amide eye drops were administered beginning on week 3 until the end of week 4 (postpartum days 15 to 30), and the rats were sacrificed at the end of week 4. Lenses were isolated and examined for oxidative stress parameters such as glutathione, lipid peroxidation, and calcium levels along with the glutathione reductase and thioltransferase enzyme activities. Casein zymography and Western blot of m-calpain were performed using the water soluble fraction of lens proteins.ResultsMorphological examination of the lenses in the NACA-treated group indicated that NACA was able to reverse the cataract grade. In addition, glutathione level, thioltransferase activity, m-calpain activity, and m-calpain level (as assessed by Western blot) were all significantly higher in the NACA-treated group than in the sodium selenite-induced cataract group. Furthermore, sodium selenite- injected rat pups had significantly higher levels of malondialdehyde, glutathione reductase enzyme activity, and calcium levels, which were reduced to control levels upon treatment with NACA.ConclusionsThe data suggest that NACA has the potential to significantly improve vision and decrease the burden of cataract-related loss of function. Prevention and reversal of cataract formation could have a global impact. Development of pharmacological agents like NACA may eventually prevent cataract formation in high-risk populations and may prevent progression of early-stage cataracts. This brings a paradigm shift from expensive surgical treatment of cataracts to relatively inexpensive prevention of vision loss.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 91%

Prevention and reversal of selenite-induced cataracts by N-acetylcysteine amide in Wistar rats

Maddirala¹,

Tobwala²,

Karacal³

et al. 2017

BMC Ophthalmol

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 91%

Prevention and reversal of selenite‐induced cataracts by N‐acetylcysteine amide in Wistar rats

Maddirala

Tobwala

Karacal

et al. 2016

Acta Ophthalmologica

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Background: The present study sought to evaluate the efficacy of N-acetylcysteine amide (NACA) eye drops in reversing the cataract formation induced by sodium selenite in male Wistar rat pups. Methods: Forty male Wistar rat pups were randomly divided into a control group, an N-acetylcysteine amide-only group, a sodium selenite-induced cataract group, and a NACA-treated sodium selenite-induced cataract group. Sodium selenite was injected intraperitoneally on postpartum day 10, whereas N-acetylcysteine amide was injected intraperitoneally on postpartum days 9, 11, and 13 in the respective groups. Cataracts were evaluated at the end of week 2 (postpartum day 14) when the rat pups opened their eyes. N-acetylcysteine amide eye drops were administered beginning on week 3 until the end of week 4 (postpartum days 15 to 30), and the rats were sacrificed at the end of week 4. Lenses were isolated and examined for oxidative stress parameters such as glutathione, lipid peroxidation, and calcium levels along with the glutathione reductase and thioltransferase enzyme activities. Casein zymography and Western blot of m-calpain were performed using the water soluble fraction of lens proteins. Results: Morphological examination of the lenses in the NACA-treated group indicated that NACA was able to reverse the cataract grade. In addition, glutathione level, thioltransferase activity, m-calpain activity, and m-calpain level (as assessed by Western blot) were all significantly higher in the NACA-treated group than in the sodium selenite-induced cataract group. Furthermore, sodium selenite-injected rat pups had significantly higher levels of malondialdehyde, glutathione reductase enzyme activity, and calcium levels, which were reduced to control levels upon treatment with NACA.

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“…One research showed intraperitoneal injection of garlic aqueous extract in rat model appeared to effectively prevent selenite-induced cataract [18] . Doganay and his colleagues demonstrated that apricots reduced selenite induced cataract in SpraqueDawley rat on the experimental cataract model [19] . The antioxidant effects and vitamin content play preventive role [20] .…”

Section: Discussionmentioning

confidence: 99%

Tavşan Modelinde Silymarinin Selenit ile Oluşturulmuş Katarakt Üzerine Koruyucu Rolü

Kabir¹,

Fattahian²,

Riazi-Esfehani³

et al. 2014

Kafkas Univ Vet Fak Derg

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The study was designed to investigate silymarine as preventive agent in selenite-induced cataract in rabbits. Eighteen rabbits were concluded and divided equally into negative control (I), positive control (II) and test (III) groups, each 6. After clinical and ophthalmoscope, bio-microscope slit lamp equipped with a digital camera and ultrasonographical examinations of rabbits' eyes, selenite sodium was administered subcutaneously on the neck in all rabbits at day 0 of the study. After induction of anesthesia, lateral recumbency and making a pore in globe 0.1ml of saline solution 0.9% in group II and 0.1ml of silymarine were injected into vitreous in group III. Any agent did not administrate in group I. Selenite sodium was injected in days third and sixth in all rabbits subcutaneously. The bio-microscopic slit lamp study showed grade one and two cataract in day 4 and 8, and grade three in day 11 in all rabbits in group I and II. In group III, three rabbits on the eighth day and four until the end of the eleventh day presented grade one cataract. Anterior lens capsule thickness was less in group III in comparison to group I and II, and posterior capsule thickness in group III was significant statistically by ultrasonography on day 20 and the anterior-posterior lens diagonal length in the experimental group comparing to the control groups was longer significantly. Selenite sodium showed cataractogenic character and silymarine as probable protective role on selenite -induced cataract in rabbit model. Keywords: Cataract, Selenite sodium, Silymarine, Rabbit Tavşan Modelinde Silymarinin Selenit ile Oluşturulmuş Katarakt Üzerine Koruyucu Rolü ÖzetBu çalışma tavşanlarda selenit ile oluşturulmuş katarakt üzerine önleyici ajan olarak silymarinin araştırılması için tasarlanmıştır. Her biri altılı gruplardan oluşan 18 tavşan negatif kontrol (I), pozitif kontrol (II) ve test (III) grupları olmak üzere eşit şekilde ayrıldı. Tavşan gözlerinin klinik ve oftalmoskop, dijital kameralı biyo-mikroskop yarık (slit) lamba ve ultrasonografik muayene sonrası sodyum selenit çalışmanın 0. gününde tüm tavşanlara boyuna subkutan olarak uygulandı. Anestezinin başlatılmasından sonra, yan yatırılarak ve küre üzerine gözenek oluşturularak 0.1ml %0.9'luk tuz solusyonu grup II'deki ve 0.1ml silymarin grup III'deki hayvanların vitreouslarına enjekte edildi. Grup I'deki hayvanlara ise herhangi bir madde enjeksiyonu yapılmadı. Selenit sodyum subkutan olarak tüm tavşanlara üçüncü ve altıncı günde enjekte edildi. Biyo-mikroskop yarık (slit) lamba çalışması birinci ve ikinci derece kataraktın 4. ve 8. günlerde ve üçüncü derece kataraktın ise 11.günde grup I ve II'deki tüm tavşanlarda gözlemlendiğini ortaya koydu. Grup III'de, üç tavşanda sekizinci günde ve dördünde ise onbirinci günün sonunda birinci derece katarakt gözlemlendi. Yirminci gündeki ultrasonografiye gore anterior lens kapsül kalınlığı, Grup I ve II 'ye göre grup III daha az ve posterior lens kapsül kalınlığı grup III'de istatistiksel olarak anlamlı bulundu. Anterior-posterior lens diagona...

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The effect of apricots on the experimental cataract model formed by sodium selenite

Cited by 8 publications

References 30 publications

Prevention and reversal of selenite-induced cataracts by N-acetylcysteine amide in Wistar rats

Prevention and reversal of selenite-induced cataracts by N-acetylcysteine amide in Wistar rats

Prevention and reversal of selenite‐induced cataracts by N‐acetylcysteine amide in Wistar rats

Tavşan Modelinde Silymarinin Selenit ile Oluşturulmuş Katarakt Üzerine Koruyucu Rolü

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