The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites

Qi, Nanshan; Liao, Shenquan; Abuzeid, Asmaa M.I.; Li, Juan; Wu, Caiyan; Lv, Minna; Lin, Xuhui; Hu, Junjing; Yu, Linzeng; Xiao, Wenwan; Sun, Mingfei; Li, Guoqing

doi:10.1038/s41598-019-41947-y

Cited by 7 publications

(2 citation statements)

References 29 publications

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“…ATG8 in E. tenella sporozoites activates upon starvation [90]. Deletion of another autophagy protein in T. gondii (ATG3) inhibits conjugation of ATG8 to the autophagosome, thereby disrupting mitochondrial homeostasis and normal growth of tachyzoites [91].…”

Section: Plos Onementioning

confidence: 99%

Dynamically expressed genes provide candidate viability biomarkers in a model coccidian

et al. 2021

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Eimeria parasites cause enteric disease in livestock and the closely related Cyclospora cayetanensis causes human disease. Oocysts of these coccidian parasites undergo maturation (sporulation) before becoming infectious. Here, we assessed transcription in maturing oocysts of Eimeria acervulina, a widespread chicken parasite, predicted gene functions, and determined which of these genes also occur in C. cayetanensis. RNA-Sequencing yielded ~2 billion paired-end reads, 92% of which mapped to the E. acervulina genome. The ~6,900 annotated genes underwent temporally-coordinated patterns of gene expression. Fifty-three genes each contributed >1,000 transcripts per million (TPM) throughout the study interval, including cation-transporting ATPases, an oocyst wall protein, a palmitoyltransferase, membrane proteins, and hypothetical proteins. These genes were enriched for 285 gene ontology (GO) terms and 13 genes were ascribed to 17 KEGG pathways, defining housekeeping processes and functions important throughout sporulation. Expression differed in mature and immature oocysts for 40% (2,928) of all genes; of these, nearly two-thirds (1,843) increased their expression over time. Eight genes expressed most in immature oocysts, encoding proteins promoting oocyst maturation and development, were assigned to 37 GO terms and 5 KEGG pathways. Fifty-six genes underwent significant upregulation in mature oocysts, each contributing at least 1,000 TPM. Of these, 40 were annotated by 215 GO assignments and 9 were associated with 18 KEGG pathways, encoding products involved in respiration, carbon fixation, energy utilization, invasion, motility, and stress and detoxification responses. Sporulation orchestrates coordinated changes in the expression of many genes, most especially those governing metabolic activity. Establishing the long-term fate of these transcripts in sporulated oocysts and in senescent and deceased oocysts will further elucidate the biology of coccidian development, and may provide tools to assay infectiousness of parasite cohorts. Moreover, because many of these genes have homologues in C. cayetanensis, they may prove useful as biomarkers for risk.

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Section: Plos Onementioning

confidence: 99%

Dynamically expressed genes provide candidate viability biomarkers in a model coccidian

et al. 2021

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“…Qi Nanshan et al also proved that starvation and the autophagy inducer rapamycin ( RAPA ) could induce autophagy in E. tenella sporozoites, while 3-Methyladenine ( 3-MA ) could inhibit sporozoite autophagy. RAPA induced sporozoite autophagy could enhance sporozoite invasion into chick embryo fibroblasts, while 3-MA inhibited sporozoite autophagy and reduced sporozoite invasion ( Qi et al, 2019 ). Zhang (2020b) found that the expression of Beclin-1 mRNA and LC3-II protein was significantly increased after E. tenella infection of chick embryo cecal epithelial cells .…”

Section: Introductionmentioning

confidence: 99%

Pathogenic mechanism of Eimeria tenella autophagy activation of chicken embryo cecal epithelial cells induced by Eimeria tenella

Duan¹,

Zhao²,

Cui³

et al. 2023

Poultry Science

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Effect of different floatation solutions on E. tenella oocyst purification and optimization of centrifugation conditions for improved recovery of oocysts and sporocysts

Liao²,

Abuzeid

et al. 2020

Experimental Parasitology

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The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites

Cited by 7 publications

References 29 publications

Dynamically expressed genes provide candidate viability biomarkers in a model coccidian

Dynamically expressed genes provide candidate viability biomarkers in a model coccidian

Pathogenic mechanism of Eimeria tenella autophagy activation of chicken embryo cecal epithelial cells induced by Eimeria tenella

Effect of different floatation solutions on E. tenella oocyst purification and optimization of centrifugation conditions for improved recovery of oocysts and sporocysts

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