The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration

Dąbrowska, Krystyna; Skaradziński, Grzegorz; Jończyk, Paulina; Kurzępa, Aneta; Wietrzyk, Joanna; Owczarek, Barbara; Żaczek, Maciej; Świtała-Jeleń, Kinga; Boratyński, Janusz; Poźniak, Gryzelda; Maciejewska, Magdalena; Górski, Andrzej

doi:10.1186/1471-2180-9-13

Cited by 16 publications

(25 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, using the s-ARU method, we showed that bacteriophage T4 and M13 exhibited the anti-migratory effect on both PC3 and LNCaP cell lines, but bacteriophage M13 appreciably interfered in cell migration rates. The results of this study also corroborate the work of Gorski's group, in which they have studied the effect of bacteriophages on the migration activity of melanom with the help of purified T4 and HAP1 bacteriophages (Dabrowska et al 2009;Kurzępa-Skaradzińska et al 2013). They have demon- Fig.…”

Section: Discussionsupporting

confidence: 89%

“…We then applied our s-ARU method in another experiment. We were interested in evaluating the effects of bacteriophage T4 and M13 on prostate cancer cells migration and proliferation, as were previously reported by Dabrowska et al (2009) for melanoma cells and by Kurzępa-Skaradzińska et al (2013) for leukemia cells. Here we performed a gap closure assay by exposing LNCaP cell line to two bacteriophages T4 and M13 using s-ARU method.…”

Section: Resultsmentioning

confidence: 95%

See 1 more Smart Citation

Development of pipette tip gap closure migration assay (s-ARU method) for studying semi-adherent cell lines

Sanmukh

Felisbino

2018

Cytotechnology

View full text Add to dashboard Cite

This work presents a pipette tip gap closure migration assay prototype tool (semi-adherent relative upsurge-s-ARU-method) to study cell migration or wound healing in semi-adherent cell lines, such as lymph node carcinoma of the prostate (LNCaP). Basically, it consists of a 6-well cover plate modification, where pipette tips with the filter are shortened and fixed vertically to the inner surface of the cover plate, with their heights adjusted to touch the bottom of the well center. This provides a barrier for the inoculated cells to grow on, creating a cell-free gap. Such a uniform gap formed can be used to study migration assay for both adherent as well as semi-adherent cells. After performing time studies, effective measurement of gap area can be carried out conveniently through image analysis software. Here, the prototype was tested for LNCaP cells, treated with testosterone and flutamide as well as with bacteriophages T4 and M13. A scratch assay using PC3 adherent cells was also performed for comparison. It was observed that s-ARU method is suitable for studying LNCaP cells migration assay, as observed from our results with testosterone, flutamide, and bacteriophages (T4 and M13). Our method is a low-cost handmade prototype, which can be an alternative to the other migration assay protocol(s) for both adherent and semi-adherent cell cultures in oncological research along with other biological research applications.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Resultsmentioning

confidence: 95%

Development of pipette tip gap closure migration assay (s-ARU method) for studying semi-adherent cell lines

Sanmukh

Felisbino

2018

Cytotechnology

View full text Add to dashboard Cite

show abstract

“…Another anticancer effect of phages may depend on their ability to enhance antitumor responses initiated by dendritic cell based vaccines [35]. We demonstrated that migration of human and mouse melanoma cells on fibronectin was inhibited by purified phage T4 and HAP1 preparations, an effect independent of the residual LPS [67]. Subsequently, staphylococcal phage lysates have also been shown to inhibit melanoma cell migration in vitro [68].…”

Section: Anticancer Effects Of Phagesmentioning

confidence: 85%

Phages and Immunomodulation

et al. 2017

Self Cite

View full text Add to dashboard Cite

In the past years, the microbiome and its role in the pathophysiology of diseases have gained great interest. The progress of our knowledge in this field opens completely novel prospects for treating disorders, including those which are most challenging to medicine today. Of special interest are studies on the interactions of the microbiome with the immune system. Only recently has the presence of bacteriophages in the microbiome been highlighted, and their potential role in maintaining normal immunity has gained increasing attention. We summarize the available data pointing to the potential impact of phages in maintaining immunological homeostasis.

show abstract

“…An in vitro study by Krystyna et al, where the migration of melanoma cells on fibronectin was shown to be inhibited by T4 phage and its substrain HAP1 phage preparations. These interactions could possibly be utilized for antibacterial therapies in cancer patients [ 5 ]. Also, the studies on possible interactions between phages and immune cells especially the phagocytes of innate immunity have shown that they do not down regulate the phagocytosis [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the bacteriophages binding to human matrix molecules

Porayath

Salim

Veedu

et al. 2018

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Recent literature has suggested a novel symbiotic relationship between bacteriophage and metazoan host that provides antimicrobial defense protecting mucosal surface by binding to host matrix mucin glycoproteins. Here, we isolated and studied different bacteriophages that specifically interact with human extracellular matrix molecules such as fibronectin, gelatin, heparin and demonstrated their potency for protection to host against microbial infections. We showed that subpopulations of bacteriophages that work against clinical isolates of Escherichia coli can bind to pure gelatin, fibronectin and heparin and reduced bacterial load in human colon cell line HT29. The bacteriophages were characterized with respect to their genome sizes, melting curve patterns and host tropism (cross-reactivity with different hosts). Since, the bacteriophages are non-toxic to the host and can effectively reduce bacterial load in HT29 cell line their therapeutic potency against bacterial infection could be explored.

show abstract

The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration

Cited by 16 publications

References 17 publications

Development of pipette tip gap closure migration assay (s-ARU method) for studying semi-adherent cell lines

Development of pipette tip gap closure migration assay (s-ARU method) for studying semi-adherent cell lines

Phages and Immunomodulation

Characterization of the bacteriophages binding to human matrix molecules

Contact Info

Product

Resources

About