The Effect of Bovine Serum Albumin on the Binding Constant and Stoichiometry of Biocompatible Magnetic Fluids

Study on interaction between poly(amidoamine) dendrimer and CdSe nanocrystal in chloroform

“…According to that approach the quantum dot PL intensity (F ) scales with the PAMAM dendrimer concentration ([PAMAM]) through the following: Table 1. Similar studies based on the formalism presented above have been successfully used to evaluate the binding properties of photosensitizes and bioluminescent compounds to biological macromolecules [20][21][22].…”

“…Analyses of the photoluminescence data were performed using the method of A.C. Tedesco et al [20][21][22]. According to that approach the quantum dot PL intensity (F ) scales with the PAMAM dendrimer concentration ([PAMAM]) through the following: Table 1.…”

“…The photoluminescence (PL) assays have been chosen due to the high sensitivity of the technique in probing the photoluminescence (PL) of CdSe nanocrystal, which is quenched by the binding of dendrimer. This provides a strategy to investigate the interaction between semiconductor nanocrystals and dendrimer through the evaluation of specific parameters that clearly describe the binding process, i.e., the binding constant and the binding capacity of the dendrimer-CdSe complex [20,21]. Furthermore, we demonstrate a self-assembled strategy that QDs coated with anion were immobilized on dendrimer surface in discrete patterns using PAMAM dendrimer as the linkers.…”

Analytical strategies for detecting nanoparticle–protein interactions

“…Besides binding affinity, fluorescence spectroscopy can also be used to measure the number of binding sites, binding constant and the degree of cooperativity of particle-protein binding (Hill constant) 16, 28, 30, 32, 34 . It will be detailed later when NP-protein binding mechanism is discussed in section 2.3.2.…”

Binding of streptomycin with bovine serum albumin: Energetics and conformational aspects

“…A steady increase in BSA fluorescence intensity with increase in streptomycin concentration was used in the determination of binding constant and number of binding sites on BSA as described by Tedesco et al [33][34][35]. According to this method the BSA fluorescence intensity (F) scales up with increase in streptomycin concentration according to the following relation [36]:…”