The effect of cell cycle on GFPuv gene expression in the baculovirus expression system

Saito, Takehiko; Dojima, Takashi; Toriyama, Masaru; Park, Enoch Y.

doi:10.1016/s0168-1656(01)00398-4

Cited by 22 publications

(19 citation statements)

References 22 publications

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“…3). While others have also observed no MOI effect in synchronously infected cultures (Kioukia et al, 1995;Schopf et al, 1990), some groups have reported a dependency (Naggie and Bentley, 1998;Saito et al, 2002). Thus, this observation cannot be generalised to all cell, virus, and media combinations.…”

Section: Resultsmentioning

confidence: 79%

A physiological product‐release model for baculovirus infected insect cells

Haas

Nielsen

2005

Biotech & Bioengineering

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Existing models describe the product release from baculovirus infected insect cells as an unspecific protein leakage occurring in parallel with protein production. The model presented here shows that the observed product release of normally non-secreted proteins can be described through cell death alone. This model avoids the implicit non-physiological assumption of previous models that cells permeable to recombinant protein as well as trypan blue continue to produce protein.

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Section: Resultsmentioning

confidence: 79%

A physiological product‐release model for baculovirus infected insect cells

Haas

Nielsen

2005

Biotech & Bioengineering

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“…These may be the reasons that the infection in the G 1 phase results in more effective virus production. Saito et al (2002) made a similar conclusion that the infection in the G 1 phase of the cell cycle is more effective for recombinant expression and infection yield than in the G 2 /M phase. However, Lynn and Hink (1978) showed that the efficiency of infection in the S phase of TN-368 cells was higher than that in the G 2 phase.…”

Section: Effect Of Growth Phase Of Suspension Culture On Cell Viabilimentioning

confidence: 77%

“…In the BEVS, TN-368 cells synchronized in the S phase were more susceptible to AcMNPV infection than cells exposed in the G 2 /M phase (Lynn and Hink, 1978). The green fluorescent protein expression corresponded to the profile of the G 1 cell cycle in the BEVS and the infection yield at G 1 or S phase-infection was 1.5-1.8-fold higher than that at G 2 /M phase-infection (Saito et al, 2002). An increased viral genome replication and recombinant protein production were observed when the infection occurred at later stages of the cell cycle (Haas et al, 2005), which is different from Saito΄s results (2002).…”

Section: Introductionmentioning

confidence: 91%

“…Through the quantitative relationship of recombinant protein expression and/or virus replication with the profile of the cell cycle in the BEVS (Saito et al, 2002), a mathematical model could be established. This model would predict and simulate the practical process of recombinant protein and/or baculovirus insecticide production according to the measured cell cycle phase distribution cluded that Sf9 cells have higher viability and stronger ability to replicate the baculovirus when infected in the exponential phase than in other growth phases.…”

Section: Effect Of Growth Phase Of Suspension Culture On Cell Viabilimentioning

confidence: 99%

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The cell cycle phase affects the potential of cells to replicate Autographa californica multiple nucleopolyhedrovirus

Zhang¹,

W²,

Xu³

et al. 2012

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Summary. -We investigated the effect of growth phase of suspension culture of insect Sf9 cells on cell cycle phase distribution, cell viability and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) production. The cell culture showed a maximum cell viability and potential to replicate the virus at the peak of G 1 phase cells in the culture, while the minimum cell viability coincided with the peak of G 2 /M phase cells. These results indicate that the G 1 phase plays a substantial role in the ability of cells to replicate the baculovirus and may help to develop a baculovirus infection dynamics model and control the expression of foreign genes.Keywords: cell cycle; baculovirus; viability; flow cytometry; infection E-mail: yhz@mail.wit.edu.cn; phone: +86-13871114386. Abbreviations: AcMNPV = Autographa californica multiple nucleopolyhedrovirus; BEVS = baculovirus expression vector system; FCM = flow cytometry; NOV = non-occluded virus; OV = occluded virus; TOI = time of infection

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“…Baculovirus was able to produce a "viral S phase," which is a benefit for viral replication (44). It has been reported that cells infected with baculovirus were arrested in the S or G 2 /M phase (5,23,47). It is therefore possible that Ac34 is required to optimize the cellular environment and functions as a regulator to help virus induce the "viral S phase" for increased viral proliferation.…”

Section: Discussionmentioning

confidence: 99%

An ac34 Deletion Mutant of Autographa californica Nucleopolyhedrovirus Exhibits Delayed Late Gene Expression and a Lack of Virulence In Vivo

Cai

Zhao

Qiu

et al. 2012

J Virol

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Ac34 and its homologs are highly conserved in all sequenced alphabaculoviruses. In this paper, we show that ac34 transcripts were detected from 6 to 48 h postinfection (p.i.) in Autographa californica nucleopolyhedrovirus (AcMNPV)-infected Sf9 cells. Ac34 localized to both the cytoplasm and the nuclei of infected cells but was not a viral structural protein. To determine the function of ac34 in the viral life cycle, an ac34 knockout AcMNPV (vAc34KO) was constructed. Compared with wild-type and repair viruses, vAc34KO exhibited an approximately 100-fold reduction in infectious virus production. Further investigations showed that the ac34 deletion did not affect the replication of viral DNA, polyhedron formation, or nucleocapsid assembly but delayed the expression of late genes, such as vp39 , 38k , and p6.9 . Bioassays revealed that vAc34KO was unable to establish a fatal infection in Trichoplusia ni larvae via per os inoculation. Few infectious progeny viruses were detected in the hemolymph of the infected larvae, indicating that the replication of vAc34KO was attenuated. These results suggest that Ac34 is an activator protein that promotes late gene expression and is essential for the pathogenicity of AcMNPV.

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The effect of cell cycle on GFPuv gene expression in the baculovirus expression system

Cited by 22 publications

References 22 publications

A physiological product‐release model for baculovirus infected insect cells

A physiological product‐release model for baculovirus infected insect cells

The cell cycle phase affects the potential of cells to replicate Autographa californica multiple nucleopolyhedrovirus

An ac34 Deletion Mutant of Autographa californica Nucleopolyhedrovirus Exhibits Delayed Late Gene Expression and a Lack of Virulence In Vivo

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