The use of the internet has increased, and it has become essential in many aspects of life, and among the other uses, e-commerce has been the most successful. The usability level can deine the criteria for judging the success of e-commerce systems after the user's interaction with these interfaces. The purpose of this paper is to compare two e-commerce systems, Amazon & Noon, in terms of satisfaction and eficiency usability, using microsoft's usability guidelines and System Usability Scale (SUS). The comparison is based on users' reviews using primary data (questionnaires). Data were collected from 120 users who participated in the two interfaces evaluation. An independent sample t-test was applied to measure both eficiency and satisfaction, and the experimental results of the t-test with 95% conidence showed that Amazon is better than Noon in terms of satisfaction and eficiency. The mean value of Amazon is 48.34, and Noon is 31.35.