The effect of dehydroepiandrosterone on renal ischemia-reperfusion-induced oxidative stress in rabbits

Abstract

Reactive oxygen species (ROS) can play an important role in the pathogenesis of ischemia-reperfusion (I/R) injury. Dehydroepiandrosterone (DHEA) is one of the hormones secreted from adrenal glands, and in some studies it has been shown that DHEA has antioxidant properties. This experimental study was designed to determine the effect of DHEA on I/R-induced oxidative stress in rabbit kidney. Twenty-one rabbits were divided into three groups. Rabbits were subjected to 60 min of left renal pedicle occlusion followed by 24 h of reperfusion. DHEA (50 mg/kg) (I/R + DHEA group) or equal volume of vehicle (I/R group) was administered 3 h prior to ischemia. The control group received only laparotomy without I/R, DHEA or vehicle. At the end of the reperfusion periods, rabbits were decapitated. Renal tissues were taken for determination of malondialdehyde (MDA) levels as an indicator of lipid peroxidation and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) activities as antioxidant enzymes. In the I/R group, while renal SOD and CAT activities were significantly lower, MDA levels were significantly higher than in the I/R + DHEA group and controls. In the I/R + DHEA group, enzyme activities and MDA levels were similar to the controls. There was no significant difference in terms of renal GPX activity among the groups. DHEA may have a beneficial effect on renal tissue against oxidative damage due to I/R by preventing decreases in some antioxidant enzyme activities.

Deleterious Effects of Supplementation with Dehydroepiandrosterone Sulphate or Dexamethasone on Rat Insulin-Secreting Cells Under In Vitro Culture Condition

“…Other possible beneficial actions of DHEAS in animal models of diabetes may include elevation of glucose transporter expression by preventing cortisol-induced transporter down-regulation as well as preventing cortisol's anti-insulin and gluconeogenesic actions. In addition, the antioxidant capacity of DHEAS may suppress inflammatory damage to pancreatic b-cells in vivo, mimicking, to some extent the anti-inflammatory action of glucocorticoids (Aksoy et al 2004;Iwasaki et al 2004).…”

The effect of shock wave lithotripsy on nitric oxide and malondialdehyde levels in plasma and urine samples

“…ROS have been implicated in the pathogenesis of renal injury after I/R. 7 Free radical formation is discussed as an important mechanism of renal injury induced by SWL. 1,15,17,18 In the study of Bomanji et al 6 it was shown that SWL causes a transient decrease in renal perfusion that returns to the normal range within a definite time period and ROS are produced during this period.…”

“…We have previously demonstrated that oxygenfree radicals play a vital role in the development of renal parenchymal damage during ischemia-reperfusion (I/R) period. 7 Excess production of free radicals during ischemia or impaired antioxidant mechanisms may cause oxidative stress. Oxidative stress may induce a number of pathophysiological processes and result in cell injury.…”

Dehydroepiandrosterone treatment attenuates reperfusion injury after testicular torsion and detorsion in rats

“…The protective effect of DHEA has been shown in various forms of T/D injury, including kidney [8], muscle flap [9], and brain [10]. However, no study has investigated the role of DHEA in testicular T/D injury.…”