The effect of different solvent extraction towards antiurolithiatic properties of Euphorbhia hirta and Orthosiphon stamineus

Abstract

There are many natural remedies derived from herbs have been used as alternatives to treat kidney stones disease effectively including Euphorbia hirta L. and Orthosiphon stamineus. Therefore, the present study aims to investigate the effect of four different extraction solvents of E. hirta and O. stamineus including hexane, methanol, ethyl acetate and water to inhibit the crystallization of calcium oxalate (CaOx) in-vitro. The inhibition of crystallization was studied by using rate of CaOx aggregation assay and was determined by UV-Vis spectrophotometer. Cystone was used as positive control. The result showed the best inhibition in aggregation of CaOx was exhibited by hexane extract of E. hirta (95.78%) which had no significant difference with Cystone, and the lowest inhibition was shown by hexane extract of O. stamineus (20% ±). It could be related to the presence of higher amount of alkaloid in hexane extract of E. hirta as compared to other extracts. In conclusion, both of plant extracts showed antiurolithiatic properties by inhibiting the crystallization of calcium oxalate. However, the rate of inhibition for both plants varies depending on the type of solvent used.

Comparison of chromatographic profiles of essential oils in extracts of 13 species from Lamiaceae family

“…Для підготовки проби нами було обрано той самий розчинник (метанол), метод екстракції (струшування), концентрацію (100 мг/мл), що і для визначення ВЕТШХ профілей флавоноїдів і гідроксикоричних кислот у об'єктах Lamiaceae [7]. Метанол є менш токсичним [12,13], ніж зазвичай застосовувані розчинники для ефірних олій, такі як толуол, метиленхлорид, гексан [3,4,10,11]; і також може екстрагувати різні терпени, включаючи й монотерпеноїди [14,15]. До того ж, на відміну від толуолу, метанол не належить до групи прекурсорів, його використання не підлягає предметно-кількісному обліку.…”

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“…Ramet al studied and demonstrated Terminalia species have the ability to prevent urinary stone formation so, that small particles do not form into large particles and they are excreted from the kidney and reduce the chance of their retention in the urinary tract11 . Pauzi et al concluded that E. hirta (hexane extract) had the best inhibition result with no significant difference (p<0.05) with standard drug cystone among all type of solvents12 . Karunasree V et al reported the chloroform extracts of Semecarpusana cardium, Terminalia chebula and Tinospora cordifolia possess the antiurolithiatic activity maybe because of the non-polar active principles residing in these plants13 .…”