Studies were carried out to find out the influence of vacuum packaging on physical parameters of whole chilli and biochemical constituents in chilli seeds. Chilli fruits were stored in vacuum packed and jute bags stored at room temperature (25±2°C), cold storage (4±1°C) under both light and dark conditions for a period of 24 months. At the end of the storage period, seeds were separated from fruits and various parameters viz., moisture content, capsaicin content, ascorbic acid, carbohydrates, protein and mineral elements like Fe, P, Na and K were analyzed. It was observed that the samples stored in vacuum packed bags maintained the quality with least deterioration in all the quality parameters compared to samples stored in jute bags.