The effect of early prenatal hypercapnia on the vascular network in the chorioallantoic membrane of the chicken embryo

Verhoelst, Eva; Ketelaere, Bart De; Decuypere, Eddy; Baerdemaeker, Josse De

doi:10.1002/btpr.569

Cited by 11 publications

(12 citation statements)

References 49 publications

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“…Thus, studies that aimed at evaluating the effect of environmental factors in vascular development have taken advantage of this system in a manner that could not be achieved in mammalian systems. Exposure of CAMs to different degrees of CO2 [205] and O2 [206,68], for example, have demonstrated the impact of gases on vascular development. While hypoxia and hyperoxia will be discussed more in detail below, this constitutes a classical example of how the system can be exploited to clarify environmental effects on vascular growth.…”

Section: Cam As a Tool To Study Vascular Developmentmentioning

confidence: 99%

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

2014

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The chicken chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development, including but not restricted to gas exchange. Over the last two decades, interest in the CAM as a robust experimental platform to study blood vessels has been shared by specialists working in bioengineering, development, morphology, biochemistry, transplant biology, cancer research and drug development. The tissue composition and accessibility of the CAM for experimental manipulation, makes it an attractive preclinical in vivo model for drug screening and / or for studies of vascular growth. In this article we provide a detailed review of the use of the CAM to study vascular biology and response of blood vessels to a variety of agonists. We also present distinct cultivation protocols discussing their advantages and limitations and provide a summarized update on the use of the CAM in vascular imaging, drug delivery, pharmacokinetics and toxicology.

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Section: Cam As a Tool To Study Vascular Developmentmentioning

confidence: 99%

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

2014

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“…However, other studies showed that the gradual increase of CO 2 concentration until day 10 of incubation promoted embryonic growth, reduced incubation time, increased hatchability (Gildersleeve et al, 1983;Bruggeman et al, 2008), and improved chick quality (Tona et al, 2007;, 2008. These results were associated with the development of the circulatory system and increased production of red blood cells, allowing greater O 2 uptake and energy conservation (Tazawa et al, 2002;Decuypere et al, 2006;Habermann et al, 2008;Verhoelst et al, 2011).…”

Section: Air Quality: O 2 and Co 2 Concentrationsmentioning

confidence: 99%

Poultry Egg Incubation: Integrating and Optimizing Production Efficiency

Boleli

Morita

Matos

et al. 2016

Rev. Bras. Cienc. Avic.

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Due to its central position in the production chain, in-ovo development is influenced by pre-incubation factors that affect the quality of embryonated eggs and incubation conditions themselves, and both may influence egg hatchability and chick quality, as well as bird survival, growth performance, and phenotype in the field. The evolution of the incubation process over the years is characterized by significant scientific and technological development. Presently, the main current focuses of research are the manipulation of thermal incubation conditions, eggshell temperature, and the integrated effects of factors that influence incubation. In this context, one of the questions that needs to be asked is how effective are the current physical conditions of incubation to promote greater hatchability and better quality chicks, and higher survival and better performance in the field under adverse conditions or not. What are the new and future prospects for incubation? The purpose of this paper was to review the role of the physical agents of incubation, such as temperature, relative humidity, O 2 and CO 2 concentration, and egg turning and position from an integrated perspective, considering egg incubation as the transitional link between egg and poultry production.

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“…Furthermore, the absorption band of water at 970 nm is detectable. In previous research,8 we have observed that the density of the vascular network in the CAM, measured by the vascular fraction (VF), increases from ED 10 to 13, and from ED 13 to 16 8. Moreover, on ED 10 and 13, the hypercapnic group has a denser vascular network compared with the control group.…”

Section: Discussionmentioning

confidence: 85%

“…Increasing the CO 2 concentrations during early incubation, as in nature,7 seems to have positive effects. It affects the embryo weight, the hatching time, the time between internal pipping and hatch, and the angiogenesis in the chorioallantoic membrane (CAM) 8–11. The CAM is a well‐vascularized membrane which lies just underneath the eggshell.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, not only the amount of blood in the CAM has been considered as a parameter of the angiogenesis but also the structure of the network which changes with incubation time and conditions 8. SRS is a technique based on near infrared spectroscopy (NIRS), but instead of one source and one detector, multiple detectors are used at different distances from the source.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The potential of spatially resolved spectroscopy for monitoring angiogenesis in the chorioallantoic membrane

Verhoelst

Bamelis

Ketelaere

et al. 2011

Biotechnology Progress

Self Cite

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Over the last decade, the poultry sector has sought to develop ways to monitor chicken embryonic development as to optimize the incubation conditions. One of the parameters of development which may change under different incubation conditions is the angiogenesis in the chorioallantoic membrane (CAM). To be able to quantify these changes in the angiogenesis and detect long-term effects on health, a non-destructive technique is necessary. In this article, the first steps toward such a non-destructive technique are successfully taken. A spatially resolved spectroscopy set-up is built and tested for its potential to measure changes in angiogenesis with incubation time, and differences between a normal and hypercapnic incubation. In this first study, reflectance measurements are performed directly on the CAM as the eggshell considerably complicates the analysis. This issue should be addressed in future research to come to a really non-destructive technique. An experiment was conducted in which one group was incubated under normal conditions, and another under early prenatal hypercapnic conditions (i.e., increased CO(2) concentrations). The angiogenesis in the CAM was measured at embryonic day (ED) 10, 13, and 16. The measurements showed a clear blood spectrum with an increasing amount of blood in time, and significant differences in the reflectance as function of the source-detector distances. However, no significant differences between the hypercapnia and the control group could be detected.

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The effect of early prenatal hypercapnia on the vascular network in the chorioallantoic membrane of the chicken embryo

Cited by 11 publications

References 49 publications

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

Poultry Egg Incubation: Integrating and Optimizing Production Efficiency

The potential of spatially resolved spectroscopy for monitoring angiogenesis in the chorioallantoic membrane

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