The Effect of Extra Virgin Olive Oil and Soybean on DNA, Cytogenicity and Some Antioxidant Enzymes in Rats

Abstract

We investigated the effect of extra virgin (EV) olive oil and genetically modified (GM) soybean on DNA, cytogenicity and some antioxidant enzymes in rodents. Forty adult male albino rats were used in this study and divided into four groups. The control group of rodents was fed basal ration only. The second group was given basal ration mixed with EV olive oil (30%). The third group was fed basal ration mixed with GM (15%), and the fourth group survived on a combination of EV olive oil, GM and the basal ration for 65 consecutive days. On day 65, blood samples were collected from each rat for antioxidant enzyme analysis. In the group fed on basal ration mixed with GM soyabean (15%), there was a significant increase in serum level of lipid peroxidation, while glutathione transferase decreased significantly. Interestingly, GM soyabean increased not only the percentage of micronucleated polychromatic erythrocytes (MPCE), but also the ratio of polychromatic erythrocytes to normochromatic erythrocytes (PEC/NEC); however, the amount of DNA and NCE were significantly decreased. Importantly, the combination of EV olive oil and GM soyabean significantly altered the tested parameters towards normal levels. This may suggest an important role for EV olive oil on rodents’ organs and warrants further investigation in humans.

Oxidative stress and immunosenescence in spleen of obese mice can be reversed by 2‐hydroxyoleic acid

“…In our study, the supplementation with unsaturated fatty acids proved to be effective at improving the oxidative state of obese mice; 2-OHOA supplementation reduced both GSSG levels and the GSSG/GSH ratio, as well as MDA levels (the marker of lipid peroxidation), when compared with the OD group, while XO activity was decreased by n-3 PUFA supplementation (in relation to the OD group). Our results are in agreement with previous evidence showing that olive oil consumption can favour tissue antioxidant defence mediated by the glutathione system (De La Cruz et al 2000), reduce lipid peroxidation levels (El-Kholy et al 2014) and improve plasma antioxidant capacity (Pitsavos et al 2005). In our study, obese animals also presented higher chemotaxis, lower cytotoxic activity and lower mitogeninduced proliferation in splenic leucocytes.…”

“…), reduce lipid peroxidation levels (El‐Kholy et al . ) and improve plasma antioxidant capacity (Pitsavos et al . ).…”

Characterization of scientific studies usually cited as evidence of adverse effects of GM food/feed

“…El‐Kholy et al . () investigated the effect of extra virgin olive oil and GM soya bean in rodents. However, the study aimed to compare only olive and soya bean, instead of GM and non‐GM soya bean, so no conclusions are possible.…”

Iron Overload Effect on Serum Levels of Glucose, Insulin and HOMA-IR was Ifluenced by the Type of Fat in Female Sprague-Dawley Rats Fed High-Fat-Diets

“…However, most of the conducted studies in human and animals confirmed the olive oil role in protecting against NAFLD, decreasing blood triglycerides, increasing high-density lipoprotein, reducing insulin resistance and hepatic steatosis [47][48][49][50][51]. It has showed that consumption of diets that rich in olive oil has a profound effect on different health outcomes, specially obesity, T2DM and metabolic syndrome [52].…”