The Effect of Fatty Acids on the Respiration of Leptospira Icterohemorrhagiae

Helprin, Jerome J.; Hiatt, C. W.

doi:10.1093/infdis/100.2.136

Cited by 25 publications

(13 citation statements)

References 13 publications

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“…Even if members of the Leptospiraceae cannot synthesize fatty acids de novo and depend on the exogenous intake of these chemicals from the environment (3, 11,12,25), fatty acids are a stable phenotypic characteristic of each strain.…”

Section: Discussionmentioning

confidence: 99%

Fatty Acid Profiles, a Chemotaxonomic Key for the Classification of Strains of the Family Leptospiraceae

Cacciapuoti

Ciceroni

Barbini

1991

International Journal of Systematic Bacteriology

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The fatty acid profiles of 59 type strains representing genera, species, and serovars belonging to the family Leptospiraceae were investigated by using gas-liquid chromatography of fatty acid methyl ester (FAME) derivatives prepared from washed leptospires. The interstrain differences of the gas-liquid chromatography FAME profiles were quantified by performing a linear regression analysis in which we compared the FAME profiles of pairs of strains and expressed the results as correlation coefficients. Leptospiral strains could be differentiated into 17 FAME relatedness groups, which were characterized by both stringent similarities within a group and sharp differences between groups. In each group, a strain was selected as a FAME reference strain. FAME reference strains can be used as keys for a chemotaxonomic phenotypic classification of strains belonging to the family Leptospiraceae.In a preliminary study (4), the fatty acids of six type strains which represented the two genera (Leptospira and Leptonema) and four species (Leptospira interrogans, Leptospira biyexa, Leptospira parva , and Leptonema illini) currently recognized in the family Leptospiraceae (13, 14, 16) were investigated by using gas-liquid chromatography of fatty acid methyl ester (FAME) derivatives. The FAME profiles of pairs of strains were compared by performing a linear regression (LR) analysis (7), and the results were expressed as correlation coefficients.At the strain level, biological and technical variables (i.e., the gas chromatographic run, the chemical derivatization process, coeval seeds of the same strain, and the growth phase of the culture) did not significantly affect the FAME profiles of the strains tested (4). On the other hand, at the interstrain level, stepwise increasing differences were observed among the type strains examined (4). These results indicated that in the family Leptospiraceae, FAME profiles behave like stable fingerprints of the bacterial phenotypes and can be used to differentiate leptospiral strains.In this study the gas-liquid chromatography FAME profiles of 59 leptospira strains representing 28 parasitic and 31 saprophytic serovars were compared to determine whether these profiles could be used as a chemotaxonomic key for the classification of members of the Leptospiraceae, using the similarities and differences in the strains tested. MATERIALS AND METHODSBacterial strains. In the family Leptospiraceae, two genera (Leptospira and Leptonema) and four species (Leptospira interrogans, Leptospira bifexa, Leptospira parva, and Leptonema illini) are presently recognized (13,14,16 (24, 27) were also included in the set of 59 serovars examined. Most of the strains tested were obtained from the Leptospira Culture Collection kept at the Istituto Superiore di Sanita (2); the exceptions were the type strains of serovars navet, peru, wawain, and lyme, which were received from A. Kaufmann, Center for Infectious Diseases, Atlanta, Ga.The strains were grown in polysorbate albumin-enriched EMJH medium (9, 17). The same batch...

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Section: Discussionmentioning

confidence: 99%

Fatty Acid Profiles, a Chemotaxonomic Key for the Classification of Strains of the Family Leptospiraceae

Cacciapuoti

Ciceroni

Barbini

1991

International Journal of Systematic Bacteriology

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“…That lipid requirements were the major obstacle had been foreshadowed; defatted fraction V of blood serum no longer stimulated respiration of Leptospira icterohaemorrhagiae (Helprin & Hiatt, 1957) or supported growth of treponemes (Oyama, Steinman & Eagle, 1953). Our work confirms that of Woratz (1957) and Ivler (1960) that oleic acid esters promote growth of Leptospira.…”

Section: Discussionmentioning

confidence: 99%

Growth of Leptospira in Defined Media

Vogel

Hutner

1961

Journal of General Microbiology

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SUMMARYSerum-free chemically defined media were developed for cultivating several Leptospira strains. Leptospira canicola, Ruebeusch strain, was the main test strain. Esterified fatty acids (monoolein, monostearin, methyl palmitate, methyl oleate) replaced serum. Fe++ over a narrow range replaced haemin. Thiamine was essential. Other vitamins added to thiamine-containing medium (nicotinic acid, pantothenate, putrescine, p-hydroxybenzoic acid, vitamin B12) speeded or increased final growth. Acetate stimulated growth moderately. Ammonium sulphate was favourable over a wide concentration range.

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“…Although long-chain fatty acids appear to serve as the major carbon and energy source for Leptospir'a (10,13,27), the pathway(s) by which these substances are oxidized by these organisms has not been described. Acyl-coenzyme A (CoA) dehydrogenase activity has been reported in cell-free extracts, and fatty acid degradation by P-oxidation has been suggested (1).…”

Section: Introductionmentioning

confidence: 99%

β-Oxidation of fatty acids by Leptospira

Henneberry¹,

Cox²

1970

Can. J. Microbiol.

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The oxidation of both saturated and unsaturated fatty acids by a water isolate and a pathogenic leptospire is reported. Evidence is presented for the existence of an adenosine triphosphate dependent fatty acid activating enzyme in the water isolate, and measurement of the reactions of β-oxidation in cell-free extracts of both strains is described. It was concluded that fatty acid degradation by β-oxidation constitutes a major catabolic pathway in Leptospira.

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The Effect of Fatty Acids on the Respiration of Leptospira Icterohemorrhagiae

Cited by 25 publications

References 13 publications

Fatty Acid Profiles, a Chemotaxonomic Key for the Classification of Strains of the Family Leptospiraceae

Fatty Acid Profiles, a Chemotaxonomic Key for the Classification of Strains of the Family Leptospiraceae

Growth of Leptospira in Defined Media

β-Oxidation of fatty acids by Leptospira

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