The effect of follicle-stimulating hormone on follicular development, granulosa cell apoptosis and steroidogenesis and its mediation by insulin-like growth factor-I in the goat ovary

Yu, Yuansong; Li, Wei; Han, Zheng-Bin; Luo, Ming-Jiu; Chang, Zhong-Le; Tan, Jing-He

doi:10.1016/j.theriogenology.2003.08.001

Cited by 54 publications

(43 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4). Besides, our previous study showed that IGF-I significantly reduced the percentage of apoptotic granulosa cells cultured in vitro [17].…”

Section: Changes In Igf Expression During Follicle Atresiamentioning

confidence: 89%

“…Concentrations of progesterone (P4) and estradiol (E2) in follicular fluid in unextracted samples were determined by direct radioimmunoassay (RIA) using a commercial kit (Jiuding Biotechniques Ltd, Tianjin, China), which has been validated for goat samples [17]. A blank culture medium assay showed no cross-reactivity and values below the limit of sensitivity (0.1ng/ml for P4 and 1pg/ml for E2).…”

Section: Radioimmunoassaymentioning

confidence: 99%

See 1 more Smart Citation

Apoptosis in Granulosa cells during follicular atresia: relationship with steroids and insulin-like growth factors

et al. 2004

Self Cite

View full text Add to dashboard Cite

It is well known that during mammalian ovarian follicular development, the majority of follicles undergo atresia at various stages of their development. However, the mechanisms controlling this selection process remain unknown. In this study, we investigated apoptosis in granulosa cells during goat follicular atresia by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). The changes in the levels of steroids, insulin-like growth factors (IGFs) and IGF receptors were studied by radioimmunoassay (RIA) and semi-quantitative reverse transcription-PCR. We found that the percentage of apoptotic granulosa cells in the atretic (A) follicles was significantly higher than that in the slightly atretic (SA) and healthy (H) follicles. The level of estradiol and the ratio of estradiol to progesterone in H follicles were significantly higher than those in A follicles. On the other hand, the level of progesterone was not significantly different among these follicle types. We also found that the level of IGF-I in H follicles was higher than in SA and A follicles, whereas the amount of IGF-II did not vary significantly. The expression of IGF receptor also decreased in A follicles as compared to that in H and SA follicles. These results suggested that estradiol and IGF-I might be involved in controlling apoptosis in granulosa cells during follicular atresia.

show abstract

“…4). Besides, our previous study showed that IGF-I significantly reduced the percentage of apoptotic granulosa cells cultured in vitro [17].…”

Section: Changes In Igf Expression During Follicle Atresiamentioning

confidence: 89%

Section: Radioimmunoassaymentioning

confidence: 99%

Apoptosis in Granulosa cells during follicular atresia: relationship with steroids and insulin-like growth factors

et al. 2004

Self Cite

View full text Add to dashboard Cite

show abstract

“…This indicates that the WID system we have developed can best mimic group culture in efficiency, while allows identification of oocyte origin. Our previous studies have shown that GCs apoptosis is an underlying mechanism of follicular atresia in the goat (Yu et al 2003(Yu et al , 2004 as in other animals (Liu et al 2003). Most of the studies on the effect of GCs apoptosis on oocyte developmental competence have so far been conducted in the bovine and using group culture systems.…”

Section: Discussionmentioning

confidence: 99%

“…It is recognized that apoptotic cell death is an underlying mechanism of cell loss during follicular atresia (Tilly et al 1991, Hsueh et al 1994, Liu et al 2003, Yu et al 2003, 2004. While the detection of granulosa cells (GCs) apoptosis has been widely used to identify atretic follicles, very little evidence for its use as a marker of oocyte quality exists.…”

Section: Introductionmentioning

confidence: 99%

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

Han¹,

Lan²,

Wu³

et al. 2006

Reproduction

View full text Add to dashboard Cite

Interactive effects of granulosa cell apoptosis, follicle size, cumulus-oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system Zheng-Bin Han, Guo-Cheng Lan, Yan-Guang Wu, Dong Han, Wei-Guo Feng, Jun-Zuo Wang and Jing-He Tan AbstractUsing a well-in-drop (WID) oocyte/embryo culture system that allows identification of follicular origin, we have investigated the effects of granulosa cells (GCs) apoptosis, follicle size, cumulus-oocyte complexes (COCs) morphology, and cumulus expansion on the developmental competence of goat oocytes matured and cultured individually following parthenogenetic activation. The WID system supported oocyte maturation and embryo development to a level similar to the conventional group system. The majority of goat oocytes acquired competence for development up to the 8-16 cell stage in follicles larger than 2 mm, but did not gain the ability to form morula/blastocyst (M/Bs) until follicles larger than 3 mm in diameter. The extent of atresia affected M/Bs formation. This effect varied according to the follicle size. Cumulus expansion increased with follicle size and decreased with increasing incidence of GCs apoptosis. Oocyte developmental potential was also correlated with cumulus expansion. Regardless of the degree of follicle atresia, 73-84% of the floating cells in the follicular fluid (FF) underwent apoptosis. Correlation between floating cell density in FF and oocyte developmental potency suggests the possibility to use the floating cell density as a simple and non-invasive marker for oocyte quality. It is concluded that the developmental potential of an oocyte is determined by multifactor interactions, and multiple factors must be considered together to accurately predict the quality of an oocyte. Reproduction (2006) 132 749-758Introduction A number of studies have shown that in vitro development of pre-implantation stage mammalian embryos is better when cultured in groups (group culture) than cultured singly in microdrops (microdrop individual culture, MIC) (Gardner et al. 1994, Kato & Tsunoda 1994, Keefer et al. 1994, Moessner & Dodson 1995. However, apart from the large-scale production of embryos for experimental purposes, most commercial and human embryo culture systems require culturing individually or in small groups. The reason for this need is the small number of oocytes available (ovum pick-up (OPU) technology, single slaughtered valuable oocyte donors and human IVF) or the required identification after culture. Besides, sometimes zona-free eggs must be cultured in isolation from each other to prevent conglomeration. Therefore, the establishment of an individual culture system that is as efficient as group culture has become of prime importance. Furthermore, although there are quite a few reports on individual culture of embryos, studies on individual culture of oocytes during maturation are few and with conflicting results (Carolan et al. 1996, Hagemann et al. 1998, Jewgenow et al. 19...

show abstract

“…During a 5-day superovulatory treatment regime, increased concentrations of IGF1 in the plasma were observed in days 4 and 5 in comparison with days 1 and 2, and a positive correlation between IGF1 in the plasma and follicular fluid was found only on day 4 (Simpson et al 1994). Increased follicular IGF1 concentrations were also found in gilts and goats treated with equine chorionic gonadotrophin (eCG) and FSH respectively, while the plasma levels were not altered (Khamsi et al 2001a, Yu et al 2003, indicating that intrafollicular IGF1 concentrations are affected more by the superovulatory treatment regime than peripheral concentrations. Whether this holds true for the oviducts and uterine horns of superovulated cows is unknown at present.…”

Section: Igf1 Concentrations During Superovulationmentioning

confidence: 99%

The role of IGF1 in the in vivo production of bovine embryos from superovulated donors

Velazquez

Zaraza²,

Oropeza³

et al. 2009

Reproduction

View full text Add to dashboard Cite

IGF1 plays an important role in bovine follicular growth, acquisition of oocyte competence and embryo viability. Current data also indicate a critical role for IGF1 in both the ovarian response and the embryo yield following the superovulatory treatments. IGF1 can have either positive or negative effects on embryo viability which is related to the concentration of IGF1 induced by superovulation treatment. These effects impact either on oocyte competence or directly on the embryo. Concentrations in the physiological range appear to result in the production of higher quality embryos, mainly due to the mitogenic and the anti-apoptotic activities of IGF1. However, high superovulatory responses are associated with decreased embryo viability and a concomitant increase in apoptosis. Studies in mice suggest that this increase in apoptosis is related to the downregulation of the IGF1 receptor in the embryo associated with high IGF1 concentrations. Strategies capable of controlling the IGF1 concentrations could be one approach to improve superovulation responses. A range of possible approaches for research within the IGF system in gonadotrophin-stimulated cattle is discussed in this review, including the possible use of superovulated female cattle as an alternative animal experimental model for research on reproductive disorders in humans associated with abnormal IGF1 concentrations.

show abstract

The effect of follicle-stimulating hormone on follicular development, granulosa cell apoptosis and steroidogenesis and its mediation by insulin-like growth factor-I in the goat ovary

Cited by 54 publications

References 36 publications

Apoptosis in Granulosa cells during follicular atresia: relationship with steroids and insulin-like growth factors

Apoptosis in Granulosa cells during follicular atresia: relationship with steroids and insulin-like growth factors

Interactive effects of granulosa cell apoptosis, follicle size, cumulus–oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system

The role of IGF1 in the in vivo production of bovine embryos from superovulated donors

Contact Info

Product

Resources

About