The effect of freezing rate on the quality of striped bass sperm

Frankel, Tyler E.; Theisen, Daniel D.; Guthrie, Helen A.; Welch, G. Rickey; Woods, L. Curry

doi:10.1016/j.theriogenology.2013.01.009

Cited by 19 publications

(13 citation statements)

References 23 publications

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“…A fast cooling program (from 7.5°C to À160°C, cooling rate: 56°C/min) was efficient to cryopreserve Eurasian perch sperm. Comparable results were described by Frankel et al [7] among testing different cooling programs for cryopreservation of Striped Bass (Morone saxatilis) sperm with a controlled rate freezer. Sperm cells reached the highest motility, viability and ATP concentration frozen to À120°C with a cooling rate of 40°C/min.…”

Section: O M / L O C a T E / Y C R Y Omentioning

confidence: 52%

Comparison of two different methods in the cryopreservation of Eurasian perch (Perca fluviatilis) sperm

et al. 2015

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Section: O M / L O C a T E / Y C R Y Omentioning

confidence: 52%

Comparison of two different methods in the cryopreservation of Eurasian perch (Perca fluviatilis) sperm

et al. 2015

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“…Although high post‐thaw sperm motility and fertilization ability have been achieved in some fish species due to considerable progress in optimizing the cryoprotectant, dilution ratio, freezing rate and thaw temperature for cryopreservation, the motility and fertility levels of cryopreserved sperm were still lower those of fresh samples (Frankel et al. ; Ciereszko et al. ).…”

Section: Discussionmentioning

confidence: 99%

Retracted: Effect of Post‐Thaw Storage Time on Motility and Fertility of Cryopreserved Beluga Sturgeon (Huso huso) Sperm

Aramli

Nazari²,

Gharibi

2015

Reprod Domestic Animals

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The aim of this study was to test the influence of post-thaw storage time on the duration of sperm motility, percentage of motile sperm, and fertilization and hatching rates of fresh sperm and sperm stored for 0, 30 and 60 min at 4°C post-thawing. After being frozen in liquid nitrogen and then thawed, the percentage of motile sperm and duration of motility were not affected by 30 min of storage at 4°C, whereas a significant decline in these parameters was observed after 60 min of storage. Similarly, fertilization and hatching rates were significantly affected within 60 min of storage at 4°C, and the fertility of frozen-thawed sperm was significantly lower than that of fresh sperm. We conclude that cryopreserved sperm of beluga sturgeon could be stored for 30 min without the loss of sperm quality. This described procedure for beluga sturgeon cryopreservation is reliable and efficient and therefore can be recommended for hatchery practice after scaling up this technique.

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“…Some authors have recommended methanol and egg yolk as cryoprotectants for P. lineatus , based on the subjective motility percentage of 67.79 ± 25% and motility duration of 74.26 ± 59 s using a standard freezing system applying a “dry‐shipper” (Felizardo et al, ). Frankel et al () observed total motility of 10.06 ± 0.085% in a 40°C/min curve for post‐thawed striped bass ( Morone saxatilis ) semen, significantly better than freezing at three slower rates (10, 15 and 20°C/min), using a computerized system. Indeed, it is possible that the use of a computerized system for motility assessments alongside a controlled cooling rate pattern may provide an explanation for the differences between previous results and those observed herein.…”

Section: Discussionmentioning

confidence: 99%

“…The influence of cooling rates on cryopreservation success is a well‐known fact and has been described by several authors. Frankel et al () investigated the effect of freezing rates (10, 15, 20, and 40°C/min) on the viability of striped bass sperm ( M. saxatilis ) and concluded that the best rate was of 40°C/min. Yang, Norris, Winn, and Tiersch () observed sensitivity regarding freezing rates, with a 5°C variation (10–15°C/min), determining a significant decrease in post‐thaw motility (from 50 ± 10% to 17 ± 12%) using methanol as a cryoprotectant for Oryzias latipes sperm.…”

Section: Discussionmentioning

confidence: 99%

“…The success of sperm freezing with liquid nitrogen requires cooling rates between 10°C and 60°C/min (Harvey & Carolsfeld, 1993), and cylinders exclusively containing liquid nitrogen vapour (Carolsfeld & Harvey, 1999) ensure cooling rates in this range, but do not allow for a controlled freezing curve. Freezing curves obtained with the use of programmable bio-freezers have been increasingly applied as an innovative tool for the study of several species (Fang, Blair, Zhong, Sun, & Zhou, 2016;Frankel, Theisen, Guthrie, Welch, & Woods, 2013;Silva et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Effects of cooling rates on the quality of Prochilodus lineatus (Valenciennes, 1836) sperm

Paula

Murgas

Castro

et al. 2019

Reprod Domestic Animals

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This study aims to investigate the effect of different cooling rates on the semen cryopreservation of curimba (Prochilodus lineatus). Nineteen ejaculates were obtained from adults males and cryopreserved at 15°C/min (CR15), 30°C/min (CR30) (controlled temperature inside and outside straw, speed was stable during freezing) and direct freezing in liquid nitrogen vapour (~35.6°C/min) (CRNV). The straws were thawed and seminal parameters evaluated. DNA fragmentation through the comet assay was assessed. A fresh sperm sample was not frozen and used for analyses. Data were submitted to an analysis of variance (ANOVA), and means were compared by Scott–Knott test (p < 0.05) using the R Software. Mean motility percentage was 100%, and motility duration was 39.5 ± 5.7 s for the fresh sperm (subjective analysis); 58.9 ± 8.0% and 24.5 ± 5.7 s for CR15; 64.8 ± 4.8% and 26.5 ± 7.1 s for CR30; and 50.1 ± 16% and 25.7 ± 4.7 s for CRNV, respectively. Motility percentages were higher and equal between CR15 and CR30 compared to CRNV (p < 0.05). Some sperm motion kinetics, namely average path velocity (VAP) and straight line velocity (VAS), were higher for CR30 (p < 0.05), while curvilinear velocity (VCL) and velocity progression (PRO) were lower for CRNV (p < 0.05). Straightness (STR) and wobble (WOB) were the same among treatments (p > 0.05). Sperm morphology results indicated higher means for total morphological sperm alterations in CRNV. All cooling rates caused sperm DNA fragmentation, although CR30 provided a less harmful effect. This is the first report for cryopreserved P. lineatus sperm preserved under different controlled cooling rates. The cooling rate of 30°C/min is indicated for the cryopreservation of this fish sperm as it led to the lowest detrimental spermatozoa effects.

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The effect of freezing rate on the quality of striped bass sperm

Cited by 19 publications

References 23 publications

Comparison of two different methods in the cryopreservation of Eurasian perch (Perca fluviatilis) sperm

Comparison of two different methods in the cryopreservation of Eurasian perch (Perca fluviatilis) sperm

Retracted: Effect of Post‐Thaw Storage Time on Motility and Fertility of Cryopreserved Beluga Sturgeon (Huso huso) Sperm

Effects of cooling rates on the quality of Prochilodus lineatus (Valenciennes, 1836) sperm

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