The Effect of Gelling agent, medium pH and silver nitrate on adventitious shoot regeneration in<i>Solanum tuberosum</i>

Kaur, Amanpreet; Kumar, Anil

doi:10.1101/2020.01.03.894063

Cited by 17 publications

(11 citation statements)

References 49 publications

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“…The response of the related parameters to AgNO3 concentration assumed a more or less concentration-dependent bell-shaped curve peaked at 4 mg L −1 . With a genotype-dependent peak, the same trend was recorded for shoot regeneration in response to the increasing concentrations of AgNO3 in the potato cultivar 'Kufri Chipsona 1′ [25,26,39], tomato [40], primrose [41], oat [42], and wheat [43]. It was also observed dealing with shoot growth and chlorophyll content in potato [26] and robusta coffee [44].…”

Section: Discussionsupporting

confidence: 59%

Boosting Polyamines to Enhance Shoot Regeneration in Potato (Solanum tuberosum L.) Using AgNO3

et al. 2022

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Advancements in shoot regeneration systems support biotechnology-based tools used in the genetic improvement of plant crops. This study aims to enhance shoot regeneration in potatoes by boosting polyamine content by adding AgNO3 to the shoot regeneration medium (MS medium supplemented with 30 g L−1 sucrose, 100 mg L−1 myoinositol, and 2.25 BA mg L−1). Five concentrations of AgNO3 (2, 4, 6, 8, and 10 mg L−1) were used in addition to a control. The effect of AgNO3 on regeneration assumed a more or less concentration-dependent bell-shaped curve peaking at 4 mg L−1. Enhancements in shoot regeneration were attributed to the known role of AgNO3 as an ethylene action blocker in addition to improvements in polyamine accumulation without an increase in H2O2 content, lipid peroxidation, or DNA damage. The uncoupling of shoot regeneration and polyamine content recorded at high AgNO3 concentrations can be attributed to the consumption of polyamines to counteract the synchronized oxidative stress manifested by increases in H2O2 content, lipid peroxidation, and DNA damage.

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Section: Discussionsupporting

confidence: 59%

Boosting Polyamines to Enhance Shoot Regeneration in Potato (Solanum tuberosum L.) Using AgNO3

et al. 2022

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“…Intercellular pH is another potentially important factor that contributes to shoot organogenesis, probably by influencing the uptake of nutrients and plant growth regulators as it regulates their solubility in culture medium (Kaur and Kumar, 2020). The expressions of 52 genes that related to the intracellular pH response were substantially altered during bamboo shoot regeneration.…”

Section: Resultsmentioning

confidence: 99%

The transcriptional dynamics during de novo shoot organogenesis of Ma bamboo (Dendrocalamus latiflorus Munro): implication of the contributions of the abiotic stress response in this process

Wang

Yao

et al. 2021

The Plant Journal

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Summary De novo shoot organogenesis is an important biotechnological tool for fundamental studies in plant. However, it is difficult in most bamboo species, and the genetic control of this highly dynamic and complicated regeneration process remains unclear. In this study, based on an in‐depth analysis at the cellular level, the shoot organogenesis from calli of Ma bamboo (Dendrocalamus latiflorus Munro) was divided into five stages. Subsequently, single‐molecule long‐read isoform sequencing of tissue samples pooled from all five stages was performed to generate a full‐length transcript landscape. A total of 83 971 transcripts, including 73 209 high‐quality full‐length transcripts, were captured, which served as an annotation reference for the subsequent RNA sequencing analysis. Time‐course transcriptome analysis of samples at the abovementioned five stages was conducted to investigate the global gene expression atlas showing genome‐wide expression of transcripts during the course of bamboo shoot organogenesis. K‐means clustering analysis and stage‐specific transcript identification revealed important dynamically expressed transcription regulators that function in bamboo shoot organogenesis. The majority of abiotic stress‐responsive genes altered their expression levels during this process, and further experiments demonstrated that exogenous application of moderate but not severe abiotic stress increased the shoot regeneration efficiency. In summary, our study provides an overview of the genetic flow dynamics during bamboo shoot organogenesis. Full‐length cDNA sequences generated in this study can serve as a valuable resource for fundamental and applied research in bamboo in the future.

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“…Generally, a culture medium can be categorized as a semi-solid or liquid medium. Although the concentration of gelling agents plays a conspicuous role in regeneration efficiency [ 132 ], there are no reports for Cannabis comparing the different types and concentrations of gelling agents. Carbohydrates are essential for many species in culture and there are many different sources (sucrose, glucose, fructose, maltose, glycerol, etc.).…”

Section: Future Directionsmentioning

confidence: 99%

The Past, Present and Future of Cannabis sativa Tissue Culture

Monthony

Page

Hesami

et al. 2021

Plants

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The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies for the species. Micropropagation affords researchers and producers methods to rapidly propagate insect-/disease-/virus-free clonal plants and store germplasm and forms the basis for other biotechnologies. Despite this need, research in the area is limited due to the long history of prohibitions and restrictions. Existing literature has multiple limitations: many publications use hemp as a proxy for drug-type Cannabis when it is well established that there is significant genotype specificity; studies using drug-type cultivars are predominantly optimized using a single cultivar; most protocols have not been replicated by independent groups, and some attempts demonstrate a lack of reproducibility across genotypes. Due to culture decline and other problems, the multiplication phase of micropropagation (Stage 2) has not been fully developed in many reports. This review will provide a brief background on the history and botany of Cannabis as well as a comprehensive and critical summary of Cannabis tissue culture. Special attention will be paid to current challenges faced by researchers, the limitations of existing Cannabis micropropagation studies, and recent developments and future directions of Cannabis tissue culture technologies.

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The Effect of Gelling agent, medium pH and silver nitrate on adventitious shoot regeneration inSolanum tuberosum

Cited by 17 publications

References 49 publications

Boosting Polyamines to Enhance Shoot Regeneration in Potato (Solanum tuberosum L.) Using AgNO3

Boosting Polyamines to Enhance Shoot Regeneration in Potato (Solanum tuberosum L.) Using AgNO3

The transcriptional dynamics during de novo shoot organogenesis of Ma bamboo (Dendrocalamus latiflorus Munro): implication of the contributions of the abiotic stress response in this process

The Past, Present and Future of Cannabis sativa Tissue Culture

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