The effect of glutaraldehyde on the electrochemical behavior of polyaniline

Melo, Jorge Dantas de; Bello, Maria E.; Azevêdo, W.M. de; Souza, Jucimar M. de; Diniz, Flamarion B.

doi:10.1016/s0013-4686(98)00375-2

Cited by 28 publications

(7 citation statements)

References 21 publications

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“…The activation of the PANI surface with glutaric dialdehyde was undertaken to study the possibilities of covalent attachment of HRP to membrane. The aldehyde was reacting with the amino groups of the polymer yielding an enamine, whereas its other carbonyl reacted with the protein, providing covalent fixation of enzyme to PANI through a five-carbon-atom linker (de Melo et al, 1999). When washed with 0.1 M Na 2 HPO 4 (pH 8.5) and 0.1% Tween-20 the samples treated with GA retained only 12% of immobilized enzyme in comparison to nontreated samples.…”

Section: Resultsmentioning

confidence: 99%

Surface functionalization of porous polypropylene membranes with polyaniline for protein immobilization

Piletsky

Piletska

Bossi

et al. 2003

Biotech & Bioengineering

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Commercial porous polypropylene membranes were chemically modified with polyaniline (PANI) using ammonium persulfate as the oxidizer. The influence of polymerization conditions on the membrane properties was studied by adsorption analysis and membrane permeability. The PANI-coated polypropylene (PANI/PP) membranes possessed high affinity toward the proteins, which can be immobilized onto the membrane surface through physical adsorption or covalent immobilization. The quantity of immobilized horseradish peroxidase (HRP) and its activity depended on the quantity and quality (oxidation level) of PANI. The storage conditions for PANI/PP membranes containing immobilized HRP were studied. HRP immobilized on the PANI/PP membrane was shown to retain 70% of its activity after 3-month storage at +5 degrees C, suggesting that this material can be used for practical application, such as in bioreactors as enzyme membranes.

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Section: Resultsmentioning

confidence: 99%

Surface functionalization of porous polypropylene membranes with polyaniline for protein immobilization

Piletsky

Piletska

Bossi

et al. 2003

Biotech & Bioengineering

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“…The disappearance of PANI peaks does not necessarily imply loss of the PANI conductivity because there is still a ow of charge due to the Paper permeable ow of ions. This behaviour was explained by de Melo et al 37 They indicated that in a near neutral medium, GA reduces PANI, but that has little effect on the loss of permeability of PANI for ions. GA rather activates the transducer, thereby rendering it heterofunctional for anion exchange.…”

Section: Immunosensor Fabricationmentioning

confidence: 83%

“…The disappearance of peaks with further electrode modication with Ab denotes the successful immobilization of Ab and good compatibility of the biomolecule with the transducer. These have reportedly been made possible by high rapid reaction of GA through its carbonyl functional groups with the PANI amine groups in the transducer and those of Ab, 37,38 respectively, and the high stability of the resultant bonds between GA and the amine groups. 36 The cyclic voltammograms of each electrode modication step during the immunosensor construction conrmed the proposed mechanism scheme in Fig.…”

Section: Immunosensor Fabricationmentioning

confidence: 99%

Development of a silver functionalised polyaniline electrochemical immunosensor for polychlorinated biphenyls

2016

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“…As the activation time so does the absorption band at 1595 cm −1 , correlated to the vibration of the C=N (imine) bond, overlapping the vibration of the C=C bond of the quinoid section of PANI. Moreover, an increase in the absorption band located at 1490 cm −1 can be observed, which is associated to the benzenoid ring stretching [22], along with a reduction of the intensity of the C–C stretching from the quinoid section, which can indicate a reduction of the purified material with the increase of activation time [26]. Further activation of PVA–PANI with a more concentrated solution (2.5% GA) confirmed the reduction of the material as well as a branching between both materials (Figure S1).…”

Section: Resultsmentioning

confidence: 99%

Purification and Glutaraldehyde Activation Study on HCl-Doped PVA–PANI Copolymers with Different Aniline Concentrations

et al. 2018

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In this work, we report the synthesis and purification of polyvinyl alcohol-polyaniline (PVA–PANI) copolymers at different aniline concentrations, and their molecular (1H-NMR and FTIR), thermal (TGA/DTG/DSC), optical (UV–Vis-NIR), and microstructural (XRD and SEM) properties before and after activation with glutaraldehyde (GA) in order to obtain an active membrane. The PVA–PANI copolymers were synthesized by chemical oxidation of aniline using ammonium persulfate (APS) in an acidified (HCl) polyvinyl alcohol matrix. The obtained copolymers were purified by dialysis and the precipitation–redispersion method in order to eliminate undesired products and compare changes due to purification. PVA–PANI products were analyzed as gels, colloidal dispersions, and thin films. 1H-NMR confirmed the molecular structure of PVA–PANI as the proposed skeletal formula, and FTIR of the obtained purified gels showed the characteristic functional groups of PVA gels with PANI nanoparticles. After exposing the material to a GA solution, the presence of the FTIR absorption bands at 1595 cm−1, 1650 cm−1, and 1717 cm−1 confirmed the activation of the material. FTIR and UV–Vis-NIR characterization showed an increase of the benzenoid section of PANI with GA exposure, which can be interpreted as a reduction of the polymer with the time of activation and concentration of the solution.

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The effect of glutaraldehyde on the electrochemical behavior of polyaniline

Cited by 28 publications

References 21 publications

Surface functionalization of porous polypropylene membranes with polyaniline for protein immobilization

Surface functionalization of porous polypropylene membranes with polyaniline for protein immobilization

Development of a silver functionalised polyaniline electrochemical immunosensor for polychlorinated biphenyls

Purification and Glutaraldehyde Activation Study on HCl-Doped PVA–PANI Copolymers with Different Aniline Concentrations

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