The effect of human cumulus cells on the maturation and developmental potential of immature oocytes in ICSI cycles

Zhang, Aijun; Xu, Bufang; Sun, Yijuan; Lu, Xiaowei; Niu, Zhihong; Chang, Qian; Feng, Yun; Xu, Chen

doi:10.1007/s10815-012-9712-3

Cited by 33 publications

(20 citation statements)

References 46 publications

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“…Subsequently, IVF or intracytoplasmic sperm injection (ICSI) was performed according to the routine of our reproductive center. Similarly, embryo quality on day 3 was evaluated according to the criteria previously described (17). Available embryos were those scored as R5, and discarded embryos were those that scored as <5.…”

Section: Ivf Embryo Culture and Embryo Evaluationmentioning

confidence: 99%

Dynamic changes of histone H3 trimethylated at positions K4 and K27 in human oocytes and preimplantation embryos

Zhang

Sun

et al. 2012

Fertility and Sterility

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Section: Ivf Embryo Culture and Embryo Evaluationmentioning

confidence: 99%

Dynamic changes of histone H3 trimethylated at positions K4 and K27 in human oocytes and preimplantation embryos

Zhang

Sun

et al. 2012

Fertility and Sterility

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“…In ovarian follicles, cumulus cells and GCs communicate with oocytes to support proper oocyte growth [10], which is important for fertilization and embryonic development [11]. A previous study found that human cumulus cells do not affect oocyte nuclear maturation, but instead affect cytoplasmic maturation and subsequent embryonic development [12]. Another study showed that inhibition of cumulus cell apoptosis improves oocyte quality and the embryo development rate [13].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Mouse Germinal Vesicle Stage Oocyte Quality by Evaluating the Cumulus Layer, Zona Pellucida, and Perivitelline Space

Zhou

Liu

et al. 2014

PLoS ONE

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To improve the outcome of assisted reproductive technology (ART) for patients with ovulation problems, it is necessary to retrieve and select germinal vesicle (GV) stage oocytes with high developmental potential. Oocytes with high developmental potential are characterized by their ability to undergo proper maturation, fertilization, and embryo development. In this study, we analyzed morphological traits of GV stage mouse oocytes, including cumulus cell layer thickness, zona pellucida thickness, and perivitelline space width. Then, we assessed the corresponding developmental potential of each of these oocytes and found that it varies across the range measured for each morphological trait. Furthermore, by manipulating these morphological traits in vitro, we were able to determine the influence of morphological variation on oocyte developmental potential. Manually altering the thickness of the cumulus layer showed strong effects on the fertilization and embryo development potentials of oocytes, whereas manipulation of zona pellucida thickness effected the oocyte maturation potential. Our results provide a systematic detailed method for selecting GV stage oocytes based on a morphological assessment approach that would benefit for several downstream ART applications.

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“…ICSI was performed only in cases of severe male factor infertility or previous fertilization failure. Embryo was evaluated and scored 72 h after fertilization according to the previously described criteria [19]. Blastocyst was evaluated and scored according to the criteria presented by Gardner and Schoolcraft (1999) [20].…”

Section: Cos Protocol and Ivf/ Intracytoplasmic Sperm Injection (Icsimentioning

confidence: 99%

Transvaginal ovarian drilling followed by controlled ovarian stimulation from the next day improves ovarian response for the poor responders with polycystic ovary syndrome during IVF treatment: a pilot study

Zhou

Cheng

et al. 2020

Reprod Biol Endocrinol

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Background: Poor response patients with PCOS who are not susceptible to gonadotropin stimulation are more likely to have canceled cycles or poor clinical outcomes during IVF treatment. However, some limitations exist in the present therapies. In this study, we evaluated the effects of using the transvaginal ovarian drilling (TVOD) followed by controlled ovarian stimulation (COS) from the second day of these poor responders. Methods: During IVF, 7 poor responders with PCOS and 28 PCOS patients (14 normal and 14 high responders) were recruited. All patients received COS with the gonadotropin-releasing hormone antagonist protocol. For the poor responders, after undergoing 10 to 14 days of ovulation induction with no response, the TVOD was applied and then ovarian stimulation was performed from the next day at the same gonadotropin dose. Serum samples during COS and follicular fluid samples from the dominant follicles on the oocyte pickup (OPU) day in all three groups were collected. Besides, follicular fluid from small follicles (diameter < 1 cm) in the normal and high responders on the OPU day and those in the poor responders on the TVOD day were gathered. Hormonal levels were examined in all samples using immunometric assays. Results: All the poor responders restored ovary response after receiving TVOD. There was no significant difference in the stimulation duration, total gonadotrophin dose used and the clinical outcomes among the three groups. The body mass index, serum and follicular levels of anti-Müllerian hormone (AMH) and testosterone in poor responders were higher than those in the other two groups, and the application of TVOD significantly decreased the levels of AMH and testosterone in both serum and follicular fluid.

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The effect of human cumulus cells on the maturation and developmental potential of immature oocytes in ICSI cycles

Abstract: The presence of cumulus cells surrounding the immature oocytes during IVM before ICSI had no influence on nuclear maturation and fertilization, but leads to better subsequent embryonic development. This is perhaps mediated by an improvement in cytoplasmic maturation.

Cited by 33 publications

References 46 publications

Dynamic changes of histone H3 trimethylated at positions K4 and K27 in human oocytes and preimplantation embryos

Dynamic changes of histone H3 trimethylated at positions K4 and K27 in human oocytes and preimplantation embryos

Assessment of Mouse Germinal Vesicle Stage Oocyte Quality by Evaluating the Cumulus Layer, Zona Pellucida, and Perivitelline Space

Transvaginal ovarian drilling followed by controlled ovarian stimulation from the next day improves ovarian response for the poor responders with polycystic ovary syndrome during IVF treatment: a pilot study

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