The effect of hybrid vigor on callus induction and plant regeneration from mature embryo culture of barley (Hordeum vulgare)

Abstract

The influence of hybrid vigor on callus induction, callus weight, regeneration capacity of callus, culture efficiency and number of regenerated plants from mature embryo cultures of winter barley (Hordeum vulgare L.) hybrid was studied. A total of 15 F 1 hybrids and their parents were used for mature embryo culture. The statistical analysis of the results revealed that positive heterosis was noticed for all these characters except for callus weight. Plants regenerated in vitro were successfully established in soil.

Effect of Hybrid Vigor on Callus Induction and Regeneration of Sugarcane

“…The number of regenerated plants obtained is no way correlated to callus induction because this depends up on the friable embryogenic calli obtained from the total calli produced. These findings were agree with the previous reports in other crops like wheat (Lazar et al 1984), rye (Rakoczy-Trojanowska and Malepszy 1993) and barley (Ozgen et al 2005). Among 3 crosses between S. officinarumϫS.spontaneum, the F1 hybrids from AP1ϫAP2 and CP1ϫCP2 were found to have the best genetic background for callus induction frequency, culture efficiency, regeneration capacity and number of plants regenerated within the hybrids.…”

“…No significant differences were observed for callus weight and regeneration efficiency in the hybrids among the crosses. Ozgen et al (2005), Linacero and Vazquez (1990) and Rakoczy- Trojanowska and Malepszy (1993) were also found that the genotype of the donor plant was a very important factor in the response in vitro.…”

“…The genetic improvement is possible through conventional breeding and selection of methodologies for somatic embryogenesis and regeneration as in maize (Petolino et al 1988), wheat (Ou et al 1989) and Barley (Ozgen et al 2005). Hybrid vigor for callus induction was indicated positive in all the 12 hybrids ( according to mid and superior parents shows 32.32% and 15.6% respectively.…”

Biometric-genetic analysis of in vitro callus proliferation in rapeseed (Brassica napus)

“…The influence of genotype on in vitro growth and differentiation patterns has been reported in a number of crop plants (Henry et al, 1994). The genetic basis of in vitro response has been analyzed in several plant systems including alfalfa (Wan et al, 1988;Kielly and Bowley, 1992;Crea et al, 1995), barley (Foroughi-Wehr et al, 1982;Komatsuda et al, 1989;Özgen et al, 2005), sunflower (Encheva et al, 2004), cotton (Gawel and Robacker, 1990), maize (Nesticky et al, 1983;Tomes and Smith, 1985), petunia (Dulieu, 1991), rice (Quimio and Zapata, 1990;Abe and Futsuhara, 1991;Yan et al, 1996;Kuroda et al, 1998), rye (Rakoczy-Trojanowska andMalepszy, 1993), tomato (Frankenberger et al, 1981;Koorneef et al, 1987;Soniya et al, 2001), wheat (Lazar et al, 1984;Ou et al, 1989;De Boyser et al, 1992;Lange et al, 1995;Dornelles et al, 1997;AbdelHady, 2006;Ozbay and Özgen, 2010), eggplant (Chakravarthi et al, 2010) and pearl millet (Mythili et al, 1997). Nesticky et al (1983) noted significant reciprocal effects in the basic of combining ability and high values of reciprocal effects in corn tissue culture.…”