The Effect of <i>rhg1</i> on Reproduction of <i>Heterodera glycines</i> in the Field and Greenhouse and Associated Effects on Agronomic Traits

Brucker, E.A.; Niblack, T. L.; Kopisch‐Obuch, Friedrich J.; Diers, Brian W.

doi:10.2135/cropsci2004.0477

Cited by 36 publications

(44 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cytological studies suggest Peking type resistances share mechanisms of giant cell breakdown (pronounced necrosis and cell wall appositions) not seen in PI88788 type resistances in response to Hg type 0 (Mahalingham and Skorupska 1996). The diVerences in the mechanism of giant cell breakdown may derive from distinct alleles at rhg1 and/or other defense-associated loci (Arelli 1994;Brucker et al 2005a).…”

mentioning

confidence: 97%

Genomic analysis of the rhg1 locus: candidate genes that underlie soybean resistance to the cyst nematode

et al. 2006

View full text Add to dashboard Cite

The rhg1 gene or genes lie at a recessive or co-dominant locus, necessary for resistance to all Hg types of the soybean (Glycine max (L.) Merr.) cyst nematode (Heterodera glycines I.). The aim here was to identify nucleotide changes within a candidate gene found at the rhg1 locus that were capable of altering resistance to Hg types 0 (race 3). A 1.5 +/- 0.25 cM region of chromosome 18 (linkage group G) was shown to encompass rhg1 using recombination events from four near isogenic line populations and nine DNA markers. The DNA markers anchored two bacterial artificial chromosome (BAC) clones 21d9 and 73p6. A single receptor like kinase (RLK; leucine rich repeat-transmembrane-protein kinase) candidate resistance gene was amplified from both BACs using redundant primers. The DNA sequence showed nine alleles of the RLK at Rhg1 in the soybean germplasm. Markers designed to detect alleles showed perfect association between allele 1 and resistance to soybean cyst nematode Hg types 0 in three segregating populations, fifteen additional selected recombination events and twenty-two Plant Introductions. A quantitative trait nucleotide (QTN) [corrected] in the RLK at rhg1 was inferred that alters A87 to V87 in the context of H274 rather than N274. [corrected] Contiguous DNA sequence of 315 kbp of chromosome 18 (about 2 cM) contained additional gene candidates that may modulate resistance to other Hg-types including a variant laccase, a hydrogen-sodium ion antiport and two proteins of unknown function. A molecular basis for recessive and co-dominant resistance that involves interactions among paralagous disease-resistance genes was inferred that would improve methods for developing new nematode-resistant soybean cultivars.

show abstract

mentioning

confidence: 97%

Genomic analysis of the rhg1 locus: candidate genes that underlie soybean resistance to the cyst nematode

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Because the primary control strategies for SCN are crop rotation and planting resistant varieties, significant attention has been focused on the identification, development, and use of soybean germplasm that exhibits resistance to SCN (Diers et al, 1997;Concibido et al, 2004;Brucker et al, 2005b;Wrather and Koenning, 2009;Kim et al, 2010aKim et al, , 2011. The Rhg1 (for Resistance to Heterodera glycines) locus, sometimes in combination with Rhg4, makes the greatest contribution to resistance in the vast majority of the commercially utilized soybean cultivars that exhibit SCN resistance (Caldwell et al, 1960;Matson and Williams, 1965;Webb et al, 1995;Li et al, 2004;Brucker et al, 2005b;Tylka et al, 2012).…”

mentioning

confidence: 99%

Distinct Copy Number, Coding Sequence, and Locus Methylation Patterns UnderlieRhg1-Mediated Soybean Resistance to Soybean Cyst Nematode

Cook¹,

Bayless²,

et al. 2014

View full text Add to dashboard Cite

Copy number variation of kilobase-scale genomic DNA segments, beyond presence/absence polymorphisms, can be an important driver of adaptive traits. Resistance to Heterodera glycines (Rhg1) is a widely utilized quantitative trait locus that makes the strongest known contribution to resistance against soybean cyst nematode (SCN), Heterodera glycines, the most damaging pathogen of soybean (Glycine max). Rhg1 was recently discovered to be a complex locus at which resistance-conferring haplotypes carry up to 10 tandem repeat copies of a 31-kb DNA segment, and three disparate genes present on each repeat contribute to SCN resistance. Here, we use whole-genome sequencing, fiber-FISH (fluorescence in situ hybridization), and other methods to discover the genetic variation at Rhg1 across 41 diverse soybean accessions. Based on copy number variation, transcript abundance, nucleic acid polymorphisms, and differentially methylated DNA regions, we find that SCN resistance is associated with multicopy Rhg1 haplotypes that form two distinct groups. The tested high-copy-number Rhg1 accessions, including plant introduction (PI) 88788, contain a flexible number of copies (seven to 10) of the 31-kb Rhg1 repeat. The identified low-copy-number Rhg1 group, including PI 548402 (Peking) and PI 437654, contains three copies of the Rhg1 repeat and a newly identified allele of Glyma18g02590 (a predicted α-SNAP [α-soluble N-ethylmaleimide–sensitive factor attachment protein]). There is strong evidence for a shared origin of the two resistance-conferring multicopy Rhg1 groups and subsequent independent evolution. Differentially methylated DNA regions also were identified within Rhg1 that correlate with SCN resistance. These data provide insights into copy number variation of multigene segments, using as the example a disease resistance trait of high economic importance.

show abstract

“…In both past and recent studies with PIs, resistance encoded by rhg1 was reported as recessive (Arelli et al 2000;Brucker et al 2005) whereas previously in NILs, the rhg1 locus was reported to be co-dominant (Meksem et al 2001a) but without single-plant marker allele associations. Clearly, co-dominant and recessive roles of plant disease resistance loci are rare and unusual Nieto et al 2007).…”

Section: Dominant Recessive or Co-dominant Nature Of Rhg1mentioning

confidence: 93%

“…First, rhg1 alone among the resistance loci was necessary, but not suYcient, for resistance to all tested Hg biotypes (Concibido et al 2004). Second, some rhg1 alleles appeared to restrict seed germination Brucker et al 2005). Third, in progeny with a resistance allele at rhg1 the allele of an interacting locus on LgM was always inherited from the resistant parent (Webb et al 1995).…”

Section: Introductionmentioning

confidence: 96%

“…The diVerences in the mechanism of giant cell breakdown in Peking and PI88788 may derive from distinct alleles at rhg1 and/or other defense-associated loci (Arelli 1994;Brucker et al 2005). The rhg1 locus was repeatedly located to a sub-telomeric region of the soybean molecular linkage group G by many studies (Webb et al 1995;Chang et al 1997;Concibido et al 1997;Cregan et al 1999;Meksem et al 2001a, b).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Recombination suppression at the dominant Rhg1/Rfs2 locus underlying soybean resistance to the cyst nematode

et al. 2011

View full text Add to dashboard Cite

Host resistance to "yellow dwarf" or "moonlight" disease cause by any population (Hg type) of Heterodera glycines I., the soybean cyst nematode (SCN), requires a functional allele at rhg1. The host resistance encoded appears to mimic an apoptotic response in the giant cells formed at the nematode feeding site about 24-48 h after nematode feeding commences. Little is known about how the host response to infection is mediated but a linked set of 3 genes has been identified within the rhg1 locus. This study aimed to identify the role of the genes within the locus that includes a receptor-like kinase (RLK), a laccase and an ion antiporter. Used were near isogeneic lines (NILs) that contrasted at their rhg1 alleles, gene-based markers, and a new Hg type 0 and new recombination events. A syntenic gene cluster on Lg B1 was found. The effectiveness of SNP probes from the RLK for distinguishing homolog sequence variants on LgB1 from alleles at the rhg1 locus on LgG was shown. The resistant allele of the rhg1 locus was shown to be dominant in NILs. None of the recombination events were within the cluster of the three candidate genes. Finally, rhg1 was shown to reduce the plant root development. A model for rhg1 as a dominant multi-gene resistance locus based on the developmental control was inferred.

show abstract

The Effect of rhg1 on Reproduction of Heterodera glycines in the Field and Greenhouse and Associated Effects on Agronomic Traits

Abstract: glycines (HG) Types of SCN (

Cited by 36 publications

References 17 publications

Genomic analysis of the rhg1 locus: candidate genes that underlie soybean resistance to the cyst nematode

Genomic analysis of the rhg1 locus: candidate genes that underlie soybean resistance to the cyst nematode

Distinct Copy Number, Coding Sequence, and Locus Methylation Patterns UnderlieRhg1-Mediated Soybean Resistance to Soybean Cyst Nematode

Recombination suppression at the dominant Rhg1/Rfs2 locus underlying soybean resistance to the cyst nematode

Contact Info

Product

Resources

About